Human GAPDH / G3PDH Protein, His Tag
分子別名(Synonym)
GAPDH,GAPD,CDABP0047,OK,SW-cl.12
表達區(qū)間及表達系統(tǒng)(Source)
Human GAPDH, His Tag (GAH-H5145) is expressed from E. coli cells. It contains AA Gly 2 - Glu 335 (Accession # AAH01601).
Predicted N-terminus: Met
蛋白結(jié)構(gòu)(Molecular Characterization)
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 36.9 kDa. The protein migrates as 37-38 kDa under reducing (R) condition (SDS-PAGE).
內(nèi)毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
純度(Purity)
>95% as determined by SDS-PAGE.
制劑(Formulation)
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重構(gòu)方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存儲(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
背景(Background)
甘油醛-3-磷酸脫氫酶(GAPDH)也被稱為肽基半胱氨酸S-亞硝酰酶GAPDH,它是一種催化糖酵解第六步的酶,因此用于分解葡萄糖以獲得能量和碳分子。GAPDH具有甘油醛-3-磷酸脫氫酶和亞硝酰酶活性,因此分別在糖酵解和核功能中發(fā)揮作用。GAPDH可以參與核事件,包括轉(zhuǎn)錄、RNA轉(zhuǎn)運、DNA復制和凋亡。此外,GAPDH可以調(diào)節(jié)細胞骨架的組織和組裝。此外,GAPDH是GAIT(γ-干擾素激活的翻譯抑制劑)復合物的一個組成部分,該復合物在炎癥過程中介導干擾素-γ誘導的轉(zhuǎn)錄選擇性翻譯抑制。
關鍵字: GAPDH;GAPDH蛋白;GAPDH酶;ACRO;百普賽斯;
百普賽斯集團ACROBiosystems Group(股票代碼:301080)是成立于2010年的跨國生物科技公司,是為全球生物醫(yī)藥、健康產(chǎn)業(yè)領域提供關鍵生物試劑產(chǎn)品及解決方案的行業(yè)平臺型基石企業(yè)。2021年在創(chuàng)業(yè)板上市。百普賽斯集團業(yè)務遍布全球,橫跨亞洲、北美洲、歐洲,在中國、美國、瑞士等12個城市設有辦公室、研發(fā)中心及生產(chǎn)基地。目前累計服務客戶超6000家,與全球Top 20醫(yī)藥企業(yè)均建立了長期、穩(wěn)定的合作伙伴關系。集團旗下?lián)碛衅放艫CROBiosystems百普賽斯、bioSeedin柏思薈、Condense Capital墾拓資本和ACRODiagnostics百斯醫(yī)學等。