Biotinylated Human LY6G6D Protein, His,Avitag? (HPLC verified)

分子別名（Synonym）

lymphocyte antigen 6 family member G6D,C6orf23,G6D,LY6-D,MEGT1,NG25

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Biotinylated Human LY6G6D Protein, His,Avitag (LYD-H82Q3) is expressed from human 293 cells (HEK293). It contains AA Asn 20 - Ser 104 (Accession # O95868-1).

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag?).

The protein has a calculated MW of 12.8 kDa. The protein migrates as 14-18 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

標(biāo)記（Labeling）

Biotinylation of this product is performed using Avitag? technology.

Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白標(biāo)記度（Protein Ratio）

Passed as determined by the HABA assay / binding ELISA.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>90% as determined by SDS-PAGE.

>90% as determined by SEC-HPLC.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

LY6G6D屬于位于6號(hào)染色體上主要組織相容性復(fù)合體（MHC）III類(lèi)區(qū)域的一組白細(xì)胞抗原-6（LY6）基因。LY6超家族的成員通常含有70至80個(gè)氨基酸，包括8至10個(gè)半胱氨酸。大多數(shù)LY6蛋白通過(guò)直接參與信號(hào)轉(zhuǎn)導(dǎo)的糖基磷脂酰肌醇（GPI）錨附著在細(xì)胞表面。