Human TFPI Protein, His Tag (active enzyme)
分子別名(Synonym)
TFPI,LACI,TFPI1,EPI,TFI
表達區(qū)間及表達系統(Source)
Human TFPI, His Tag (TFI-H5226) is expressed from human 293 cells (HEK293). It contains AA Asp 29 - Lys 282 (Accession # NP_006278.1).
Predicted N-terminus: Asp 29
蛋白結構(Molecular Characterization)
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 30.0 kDa. The protein migrates as 41-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
內毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
純度(Purity)
>90% as determined by SDS-PAGE.
制劑(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
重構方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存儲(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
背景(Background)
組織因子途徑抑制物(TFPI)也稱為外源途徑抑制物(EPI)、脂蛋白相關凝血抑制物(LACI),是一種由血管內皮細胞合成的血漿蛋白酶抑制物,其中一部分與這些細胞的糖胺聚糖有關。TFPI是一種單鏈多肽,可以可逆地抑制因子Xa(Xa)和凝血酶(因子IIa)。TFPI是一種分泌蛋白,具有N端酸性區(qū)、由兩個接頭區(qū)分隔的三個Kunitz(K)結構域和一個C端堿性區(qū)。第一個K結構域抑制與組織因子(TF)復合的凝血因子VIIa;第二個K結構域抑制因子Xa;第三個K結構域與肝素結合;C終端基本區(qū)域可以具有多種功能。例如,它在TFPI與細胞表面的結合中起著重要作用。
關鍵字: TFPI;TFPI蛋白;TFPI重組蛋白;ACRO;百普賽斯;
百普賽斯集團ACROBiosystems Group(股票代碼:301080)是成立于2010年的跨國生物科技公司,是為全球生物醫(yī)藥、健康產業(yè)領域提供關鍵生物試劑產品及解決方案的行業(yè)平臺型基石企業(yè)。2021年在創(chuàng)業(yè)板上市。百普賽斯集團業(yè)務遍布全球,橫跨亞洲、北美洲、歐洲,在中國、美國、瑞士等12個城市設有辦公室、研發(fā)中心及生產基地。目前累計服務客戶超6000家,與全球Top 20醫(yī)藥企業(yè)均建立了長期、穩(wěn)定的合作伙伴關系。集團旗下擁有品牌ACROBiosystems百普賽斯、bioSeedin柏思薈、Condense Capital墾拓資本和ACRODiagnostics百斯醫(yī)學等。