Human B7-1 / CD80 Protein, Mouse IgG2a Fc Tag, low endotoxin (MALS verified)

分子別名（Synonym）

CD80,B7,B7-1,B7.1,BB1,CD28LG,CD28LG1,LAB7

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human B7-1, Mouse IgG2a Fc Tag (B71-H52A4) is expressed from human 293 cells (HEK293). It contains AA Val 35 - Asn 242 (Accession # P33681-1).

Predicted N-terminus: Val 35

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a mouse IgG2a Fc tag at the C-terminus.

The protein has a calculated MW of 50.9 kDa. The protein migrates as 65-85 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 0.1 EU per μg by the LAL method.

純度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

B7-1和B7-2及其受體CD28和CTLA4共同構(gòu)成了調(diào)節(jié)T和B細(xì)胞反應(yīng)的主要共刺激途徑之一。盡管CTLA 4和CD28都可以結(jié)合相同的配體，但CTLA 4結(jié)合B7 1和B7 2的親和力比CD28高20-100倍，并參與免疫反應(yīng)的下調(diào)。

B淋巴細(xì)胞活化抗原B7-1（簡(jiǎn)稱(chēng)B7），也稱(chēng)為分化簇80（CD80），是細(xì)胞表面免疫球蛋白超家族的成員，在活化的B細(xì)胞、活化的T細(xì)胞、巨噬細(xì)胞和樹(shù)突狀細(xì)胞上表達(dá)。它是T細(xì)胞表面兩種不同蛋白質(zhì)的配體：CD28（用于自動(dòng)調(diào)節(jié)和細(xì)胞間結(jié)合）和CTLA-4（用于減弱調(diào)節(jié)和細(xì)胞分離）。CD80與CD86協(xié)同作用以激活T細(xì)胞。CD80通過(guò)激活巨噬細(xì)胞中的NF-κB信號(hào)通路在誘導(dǎo)先天免疫反應(yīng)中發(fā)揮作用。因此，CD80被認(rèn)為是治療自身免疫性疾病和各種癌癥的有前景的靶點(diǎn)。