Human B7-1 / CD80 Protein, Mouse IgG2a Fc Tag, low endotoxin (MALS verified)

分子別名（Synonym）

CD80,B7,B7-1,B7.1,BB1,CD28LG,CD28LG1,LAB7

表達區(qū)間及表達系統(tǒng)（Source）

Human B7-1, Mouse IgG2a Fc Tag (B71-H52A4) is expressed from human 293 cells (HEK293). It contains AA Val 35 - Asn 242 (Accession # P33681-1).

Predicted N-terminus: Val 35

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a mouse IgG2a Fc tag at the C-terminus.

The protein has a calculated MW of 50.9 kDa. The protein migrates as 65-85 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 0.1 EU per μg by the LAL method.

純度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

B7-1和B7-2及其受體CD28和CTLA4共同構(gòu)成了調(diào)節(jié)T和B細胞反應的主要共刺激途徑之一。盡管CTLA 4和CD28都可以結(jié)合相同的配體，但CTLA 4結(jié)合B7 1和B7 2的親和力比CD28高20-100倍，并參與免疫反應的下調(diào)。

B淋巴細胞活化抗原B7-1（簡稱B7），也稱為分化簇80（CD80），是細胞表面免疫球蛋白超家族的成員，在活化的B細胞、活化的T細胞、巨噬細胞和樹突狀細胞上表達。它是T細胞表面兩種不同蛋白質(zhì)的配體：CD28（用于自動調(diào)節(jié)和細胞間結(jié)合）和CTLA-4（用于減弱調(diào)節(jié)和細胞分離）。CD80與CD86協(xié)同作用以激活T細胞。CD80通過激活巨噬細胞中的NF-κB信號通路在誘導先天免疫反應中發(fā)揮作用。因此，CD80被認為是治療自身免疫性疾病和各種癌癥的有前景的靶點。