Biotinylated Human DDR1 Protein, His,Avitag? (MALS verified)
分子別名（Synonym）
CD167a
表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）
Biotinylated Human DDR1, His,Avitag (DD1-H82E9) is expressed from human 293 cells (HEK293). It contains AA Asp 21 - Thr 416 (Accession # Q08345-1).
Predicted N-terminus: Asp 21
蛋白結(jié)構(gòu)（Molecular Characterization）
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag?).
The protein has a calculated MW of 47.5 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
標(biāo)記（Biotinylation）
Biotinylation of this product is performed using Avitag? technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白標(biāo)記度（Biotin:Protein Ratio)
Passed as determined by the HABA assay / binding ELISA.
內(nèi)毒素（Endotoxin）
Less than 1.0 EU per μg by the LAL method.
純度（Purity）
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
制劑（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
重構(gòu)方法（Reconstitution）
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存儲（Storage）
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
背景（Background）
Discoidin結(jié)構(gòu)域受體1（DDR1）是DDR的成員，DDR是受體酪氨酸激酶（RTK）的成員。在膠原結(jié)合后，DDR1經(jīng)歷酪氨酸自磷酸化，從而觸發(fā)下游遺傳和細(xì)胞途徑，并在細(xì)胞形態(tài)發(fā)生、分化、增殖、粘附、遷移和侵襲的調(diào)節(jié)中發(fā)揮關(guān)鍵作用。研究表明，DDR1與多種人類疾病密切相關(guān)，包括癌癥、纖維化、動脈粥樣硬化和其他炎癥性疾病。新一代DDR1抑制劑靶向規(guī)范ATP結(jié)合袋或細(xì)胞外結(jié)構(gòu)域外的變構(gòu)位點(diǎn)（變構(gòu)抑制劑），可能為選擇性DDR1抑制療法的開發(fā)提供新的機(jī)會。
關(guān)鍵字： DDR1;DDR1蛋白;DDR1重組蛋白;ACRO;百普賽斯;
百普賽斯集團(tuán)ACROBiosystems Group（股票代碼：301080）是成立于2010年的跨國生物科技公司，是為全球生物醫(yī)藥、健康產(chǎn)業(yè)領(lǐng)域提供關(guān)鍵生物試劑產(chǎn)品及解決方案的行業(yè)平臺型基石企業(yè)。2021年在創(chuàng)業(yè)板上市。百普賽斯集團(tuán)業(yè)務(wù)遍布全球，橫跨亞洲、北美洲、歐洲，在中國、美國、瑞士等12個城市設(shè)有辦公室、研發(fā)中心及生產(chǎn)基地。目前累計服務(wù)客戶超6000家，與全球Top 20醫(yī)藥企業(yè)均建立了長期、穩(wěn)定的合作伙伴關(guān)系。集團(tuán)旗下?lián)碛衅放艫CROBiosystems百普賽斯、bioSeedin柏思薈、Condense Capital墾拓資本和ACRODiagnostics百斯醫(yī)學(xué)等。