Human PD-L2 / B7-DC Protein, Fc Tag (HPLC verified)

分子別名（Synonym）

PDL2,PD-L2,Butyrophilin B7-DC,CD273,PDCD1 ligand 2,PDCD1L2,PDCD1LG2

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human PD-L2 Protein, Fc Tag (PD2-H5251) is expressed from human 293 cells (HEK293). It contains AA Leu 20 - Pro 219 (Accession # AAI13679).

Predicted N-terminus: Leu 20

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 49.2 kDa. The protein migrates as 60-70 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-HPLC.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

程序性細(xì)胞死亡1配體2（PD-L2）也被稱為B7-DC，CD273，屬于免疫球蛋白超家族或BTN/MOG家族。PD-L2的表達(dá)通過單核細(xì)胞中的IFNG/IFN-γ刺激上調(diào)，并在用CSF2和IL-4從外周血單核細(xì)胞生長的樹突狀細(xì)胞上誘導(dǎo)。PD-L2參與共刺激信號(hào)，以PDCD1非依賴的方式對(duì)T細(xì)胞增殖和IFNG產(chǎn)生至關(guān)重要。PD-L2與PDCD1的相互作用通過阻斷細(xì)胞周期進(jìn)程和細(xì)胞因子產(chǎn)生來抑制T細(xì)胞增殖。