Human Apolipoprotein E / APOE3 Protein, His Tag

分子別名（Synonym）

Apolipoprotein E,APOE,Apo-E

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human APOE3, His Tag (APE-H5246) is expressed from human 293 cells (HEK293). It contains AA Lys 19 - His 317 (Accession # P02649-1).

Predicted N-terminus: Lys 19

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 36.1 kDa. The protein migrates as 35-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

無(wú)菌（Sterility）

Negative

純度（Purity）

>90% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

APOE是一種載脂蛋白，一種與脂質(zhì)顆粒相關(guān)的蛋白質(zhì)，主要通過(guò)血漿和間質(zhì)液在脂蛋白介導(dǎo)的器官間脂質(zhì)運(yùn)輸中發(fā)揮作用。載脂蛋白E是血漿脂蛋白的核心成分，參與其產(chǎn)生、轉(zhuǎn)化和清除。載脂蛋白是兩親性分子，與脂蛋白顆粒核心的脂質(zhì)和血漿的水環(huán)境相互作用。因此，APOE與乳糜微粒、乳糜微粒殘留、極低密度脂蛋白（VLDL）和中密度脂蛋白。它還與多種細(xì)胞受體結(jié)合，包括LDL受體/LDLR、LDL受體相關(guān)蛋白LRP1、LRP2和LRP8以及極低密度脂蛋白受體/VDLR，這些受體介導(dǎo)含APOE的脂蛋白顆粒的細(xì)胞攝取。