Human TNFSF11 / RANKL / CD254 Protein, His Tag, active trimer (MALS verified)

分子別名（Synonym）

RANKL,CD254,TRANCE,OPGL,ODF

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human TNFSF11 Protein, His Tag (RAL-H5240) is expressed from human 293 cells (HEK293). It contains AA Gly 64 - Asp 245 (Accession # AAC51762.1).

Predicted N-terminus: His

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 22.4 kDa. The protein migrates as 27-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>90% as determined by SDS-PAGE.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

核因子κB配體受體激活劑（RANKL），也稱為腫瘤壞死因子配體超家族成員11（TNFSF11）、TNF相關(guān)活化誘導(dǎo)細(xì)胞因子（TRANCE）、骨保護(hù)素配體（OPGL）和破骨細(xì)胞分化因子（ODF），被稱為II型膜蛋白，是腫瘤壞死因子（TNF）超家族的成員。RANKL通過其刺激破骨細(xì)胞形成和活性的能力，是骨吸收和整體骨密度的關(guān)鍵介質(zhì)。一些研究結(jié)果還表明，一些癌癥細(xì)胞，特別是癌癥細(xì)胞，可以激活骨重塑的增加，并最終增加總的骨生成。骨重塑和骨生成的增加增加了骨轉(zhuǎn)移的整體生長(zhǎng)。骨重塑的總體控制是通過RANKL與其受體或其誘餌受體RANK和OPG的結(jié)合來調(diào)節(jié)的。