Human IGF1R / CD221 Protein, His Tag (MALS verified)

分子別名（Synonym）

IGF1R,IGFR,JTK13,CD221,MGC142170,MGC142172,MGC18216

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human IGF-I R, His Tag (IGR-H5229) is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Asn 932 (Accession # P08069-1).

Predicted N-terminus: Glu 31

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

This protein contains a furin convertase cleavage site, 737-RKRR-740, and will be partially processed into N (α chain) and C-terminal fragment (partial β chain) with calculated MW of 81.0 kDa and 23.8 kDa respectively. The protein migrates as 35-45 kDa (partial β chain), 110-120 kDa (α chain) and 130 kDa (α chain & partial β chain) under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

純度（Purity）

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

胰島素樣生長(zhǎng)因子1受體（IGF1R）也稱為CD221、JTK13。并且是被IGF-1和相關(guān)生長(zhǎng)因子IGF-2激活的跨膜受體。它屬于一大類酪氨酸激酶受體。該受體介導(dǎo)IGF-1的作用，IGF-1是一種分子結(jié)構(gòu)與胰島素相似的多肽蛋白激素。IGF1R由兩個(gè)α亞基和兩個(gè)β亞基組成，α和β亞基都是由單個(gè)信使核糖核酸前體合成的。然后將前體糖基化，蛋白水解裂解，并通過半胱氨酸鍵交聯(lián)，形成功能性跨膜αβ鏈。α鏈位于細(xì)胞外，而β亞基橫跨細(xì)胞膜，負(fù)責(zé)配體刺激下的細(xì)胞內(nèi)信號(hào)轉(zhuǎn)導(dǎo)。IGF1R具有ATP的結(jié)合位點(diǎn)，ATP用于提供用于自身磷酸化的磷酸鹽。IGF1R和胰島素受體之間有60%的同源性。作為對(duì)配體結(jié)合的反應(yīng)，α鏈誘導(dǎo)β鏈的酪氨酸自磷酸化。這一事件觸發(fā)了細(xì)胞內(nèi)信號(hào)的級(jí)聯(lián)，雖然有一定的細(xì)胞類型特異性，但通常促進(jìn)細(xì)胞存活和細(xì)胞增殖。