Human IL-4 Protein, premium grade

分子別名（Synonym）

IL4,BCGF1,BSF1

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human IL-4, premium grade (IL4-H4218) is expressed from human 293 cells (HEK293). It contains AA His 25 - Ser 153 (Accession # P05112-1).

Predicted N-terminus: His 25

Human IL-4, premium grade (IL4-H4218), designed for preclinical stage, has the same activity and performance with GMP Human IL-4 (GMP-L04H26), which enables a seamless transition from preclinical development to clinical phases. Premium Grade product offer a cost efficient alternative of GMP Grade products for the early development phase when safety of raw materials is not top priority. By using Premium Grade products in early development phase, you can transition easily into clinical and commercial phase without need to revalidate the raw materials and modify manufacturing process.

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries no "tag".

The protein has a calculated MW of 15.0 kDa. The protein migrates as 19 kDa±3 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 0.01 EU per μg by the LAL method.

宿主蛋白殘留（Host Cell Protein）

<0.5 ng/μg of protein tested by ELISA.

宿主核酸殘留（Host Cell DNA）

<0.02 ng/μg of protein tested by qPCR.

無菌（Sterility）

Negative

支原體（Mycoplasma）

Negative.

純度（Purity）

>95% as determined by SDS-PAGE.

>95% as determined by SEC-HPLC.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

白細(xì)胞介素-4是一種誘導(dǎo)幼稚輔助性T細(xì)胞（Th0細(xì)胞向Th2細(xì)胞）分化的細(xì)胞因子。在存在IL-4和IL-13（Th-2型反應(yīng)中產(chǎn)生的細(xì)胞因子，特別是在過敏和寄生蟲感染期間）的情況下，巨噬細(xì)胞被差異激活，并且該細(xì)胞因子是白細(xì)胞介素4受體的配體。白細(xì)胞介素4受體也與IL13結(jié)合，這可能有助于該細(xì)胞因子和IL13的許多重疊功能。STAT6是一種信號轉(zhuǎn)導(dǎo)和轉(zhuǎn)錄激活因子，已被證明在介導(dǎo)這種細(xì)胞因子的免疫調(diào)節(jié)信號中起著核心作用。最近，研究人員發(fā)現(xiàn)，細(xì)胞因子IL-4在幾種基因缺陷小鼠品系（包括Itk，KLF2，CBP和Id3）的胸腺中先天CD8+T細(xì)胞的發(fā)育中起關(guān)鍵作用，而之前沒有暴露于抗原。