Human IL-2 Protein, Tag Free (MALS verified)

分子別名（Synonym）

IL2,TCGF,lymphokine,Interleukin 2

表達(dá)區(qū)間及表達(dá)系統(tǒng)（Source）

Human IL-2 Protein, Tag Free (IL2-H5215) is expressed from human 293 cells (HEK293). It contains AA Ala 21 - Thr 153 (Accession # P60568-1).

Predicted N-terminus: Ala 21

蛋白結(jié)構(gòu)（Molecular Characterization）

This protein carries no "tag".

The protein has a calculated MW of 15.4 kDa. The protein migrates as 14 kDa and 16-17 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

內(nèi)毒素（Endotoxin）

Less than 0.01 EU per μg by the LAL method.

宿主蛋白殘留（Host Cell Protein）

<0.5 ng/μg of protein tested by ELISA.

宿主核酸殘留（Host Cell DNA）

<0.02 ng/μg of protein tested by qPCR.

無菌（Sterility）

Negative

支原體（Mycoplasma）

Negative.

純度（Purity）

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

-20°C to -70°C for 12 months in lyophilized state;

-70°C for 3 months under sterile conditions after reconstitution.

背景（Background）

白細(xì)胞介素-2（IL-2）是一種白細(xì)胞介素，一種細(xì)胞因子免疫系統(tǒng)信號(hào)分子，是一種白細(xì)胞營養(yǎng)激素，有助于身體對微生物感染的自然反應(yīng)，并區(qū)分外來（非自我）和自我。IL-2通過與負(fù)責(zé)免疫的淋巴細(xì)胞表達(dá)的IL-2受體結(jié)合來介導(dǎo)其作用。成熟的人IL-2分別與小鼠和大鼠IL-2具有56%和66%的氨基酸序列同一性。人和小鼠IL-2表現(xiàn)出跨物種活性。IL-2受體由三個(gè)亞基組成，這些亞基以不同的預(yù)先形成的復(fù)合物存在于細(xì)胞表面。在胸腺T細(xì)胞發(fā)育過程中，IL-2對于稱為調(diào)節(jié)性T細(xì)胞（T-regs）的獨(dú)特T細(xì)胞亞群的成熟也是必需的。T-Regs通過阻止反應(yīng)細(xì)胞產(chǎn)生IL-2來實(shí)現(xiàn)。因此，IL-2需要區(qū)分自我和非自我，這是免疫系統(tǒng)的另一個(gè)獨(dú)特特征。