Human Laminin 521 Protein, premium grade

產(chǎn)品描述（Product Details）

Laminin 521 (LN521) is a recombinant human protein that provides a defined surface for in vitro feeder-free culture of multiple human pluripotent stem cells (PSCs). LN521 has been proven to maintain normal growth characteristics and stemness in multiple PSC lines without simultaneous differentiation, which includes ESC, iPSC, MSC etc. In addition, LN521 has been demonstrated to support PSC growth for >10 passages without any signs of karyotypic abnormalities and to maintain the ability of PSCs to differentiate into all three germ line lineages.

Flexible & Compatible

Laminin 521 could work well in any commercial stem cell media. Meanwhile, it could support the attachment and expansion of hPSCs both in single cells or small colonies.

Stemness maintenance 

Laminin 521 is the biologically relevant hPSCs extracellular matrix. It is crucial for the growth and stemness maintenance of hPSCs in human through its binding to cell receptors a6β1 integrin.

Enhance cell differentiation

Due to the diverse biorelevant environment, laminin 521 could also enhance cell differentiation, polarization and organization of target cell types, including neuron, hepatocytes, cardiomyocytes, retinal pigmented epithelial cells, pancreatic β-cells and so on.

Reduce Variability

LN521 is a defined, recombinant human protein with better lot-to-lot consistency that reduces variability in your PSC cultures.

產(chǎn)品參數(shù)（Key parameter）

重構(gòu)方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

制劑（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

存儲(chǔ)（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 24 months in lyophilized state;

2. -70°C for 3 months under sterile conditions after reconstitution.

Laminin 521分子背景

Laminin 521 were expressed in Descemet's membrane and corneal endothelium. This laminin isoform significantly enhanced the in vitro adhesion and proliferation, and differentiation of HCECs. Laminin 511-E8 consists of the C-terminal end of the alpha 5, beta 2, and gamma 1 chains.