IdeS產品信息
產品名稱：IgG降解蛋白酶
產品描述（Product Details）
This product is derived from Streptococcus pyogenes and expressed in E. coli, it is an IgG-specific cysteine protease that digests antibodies at a single amino acid site below the hinge, generating homogenous F(ab’)2 and Fc fragments.
應用說明（Application）
IdeS cleaves IgG to produce F(ab')2 and Fc fragments at specific site.
單位定義（Unit Definition）
One unit digests ≥ 95% of 1 μg human IgG when incubated in 10 mM sodium phosphate, 137 mM NaCl, 2.7 mM KCl, pH7.4 at 37°C for 30min.
質量控制（Quility Control）
Less than 1.0 EU per μg by the LAL method. >95% as determined by SDS-PAGE.
純度（Purity）
>95% as determined by SDS-PAGE.
制劑（Formulation）
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
存儲（Storage）
This product is stable after storage at:
1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.
IdeS分子背景
IdeS from Streptococcus pyogenes, also known as Mac-1, is an endopeptidase with specificity for IgG. The bacterial protein Mac-1 adopts an alpha/beta fold, with 14 beta strands and 9 alpha helices. The N-terminal domain is made up predominantly of alpha helices, whereas the C-terminal domain consists predominantly of beta sheets. Mac 1 blocks polymorphonuclear opsonophagocytosis, inhibits the production of reactive oxygen species and contains IgG endopeptidase activity.IdeS is a unique enzyme that digests IgG at a specific site below the hinge region, generating a homogenous pool of F(ab‘)2 and Fc/2 fragments. IdeS digests all subclasses of human, and some classes of monkey, rabbit and sheep IgG. It has limited activity on mouse IgG2a and IgG3 - for digestion of these antibody species. IdeS is cloned from Streptococcus pyogenes and expressed in E. coli.
關鍵字： IdeS;IgG降解蛋白酶;IdeS酶;降解蛋白酶;IgG蛋白酶;
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