CD73產(chǎn)品信息
英文名稱:5'-nucleotidase
中文名稱:5'-核苷酸酶
靶點(diǎn)別稱:CD73,NT5E,5'-Nucleotidase,5'-NT,NT5,NTE
物種:Human / Mouse / Cynomolgus / Rat / Rabbit / Canine / Sus scrofa (Pig)
屬性:Protein / kits / Beads
標(biāo)記:Biotin-labeled / Unconjugated
內(nèi)毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
純度(Purity)
>90% as determined by SDS-PAGE.
>90% as determined by SEC-HPLC.
制劑(Formulation)
Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 120 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重構(gòu)方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存儲(chǔ)(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
1. -20°C to -70°C for 24 months in lyophilized state;
2. -70°C for 12 months under sterile conditions after reconstitution.
CD73分子背景
5'-核苷酸酶(5'-NT),也稱為胞外-5'-核苷酸酶或CD73(分化簇73),是由NT5E基因編碼的酶。CD73通常用于將AMP轉(zhuǎn)化為腺苷。外切5-三核苷酸酶(5-核糖核苷酸磷酸水解酶)在中性pH下催化嘌呤5-三核苷酸轉(zhuǎn)化為核苷,優(yōu)選的底物是AMP。其他形式的5-三聚核苷酸酶存在于細(xì)胞質(zhì)和溶酶體中,可以通過其底物親和力、對(duì)二價(jià)鎂離子的需求、ATP的激活和無(wú)機(jī)磷酸鹽的抑制與胞外NPT5區(qū)分開來(lái)。罕見的等位基因變異與成人發(fā)病的關(guān)節(jié)和動(dòng)脈鈣化綜合征(CALJA)有關(guān),該綜合征影響髂、股和脛動(dòng)脈,減少腿部和手腳關(guān)節(jié)的循環(huán),導(dǎo)致疼痛。
CD73用戶評(píng)價(jià)
關(guān)鍵字: CD73;CD73蛋白;5'-nucleotidase;NT5E;5'-核苷酸酶;
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