中文名：核糖核酸酶T1 來源于米曲霉(色譜純化,凍干)
英文名：Ribonuclease T1 from Aspergillus oryzae(Chromatographically Purified,Lyophilized)
中文別名：RNase T1;Guanyloribonuclease;Ribonucleate 3′-guanylo-oligonucleotidohydrolase
英文別名：RNase T1;Guanyloribonuclease;Ribonucleate 3′-guanylo-oligonucleotidohydrolase
純度：≥2,500 units/mg
貨號：R128612
包裝：2500ku、100ku、500ku
Cas號：9026-12-4
存儲溫度：2-8°C儲存
產(chǎn)品介紹：
Ribonuclease T1 is an endoribonuclease, highly specific for the cleavage of RNA or deaminated RNA between guanosine 3'-phosphate residues (or inosine 3'-phosphate) and the 5'-OH residues of adjacent nucleotides with the formation of the corresponding intermediate 2', 3'-cyclic phosphates. It cleaves single-stranded RNA releasing oligonucleotides from the guanosine 3'-phosphate termini. The enzyme has a molecular weight of 11 kDa. The optimum pH is 7.5. RNase T1 is inhibited by Ag+, Zn2+, Cu2+, and Hg2+ at 1 X 10-3 M. The stimulatory effects of both histidine and EDTA are attributed to chelation of contaminating inhibitor cations. The enzyme assay is essentially the method of Egami et al., Prog. in Nucleic Acid Res. and Molec. Biol., III, 59 (1964) based upon the release of acid soluble oligonucleotides following the digestion of yeast RNA.Ribonuclease T1 (RNase T1) from Aspergillus oryzae is used to digest denatured RNA prior to sequencing and is used for protein folding studies .
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關(guān)鍵字： RNase T1;Guanyloribonuclease;Ribonucleate 3′-guanylo-oligonucleotidohydrolase;RNase T1;Guanyloribonuclease;Ribonucleate 3′-guanylo-oligonucleotidohydrolase
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