Rabbit Anti-phospho-Smad3 (Ser423) Polyclonal Antibody
DATASHEET
Host:Rabbit
Target Protein:phospho-Smad3 (Ser423)
IR:Immunogen Range:CS(p-S)VS
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:4088
Swiss Prot:P84022
Source:KLH conjugated Synthesised phosphopeptide derived from human Smad3 around the phosphorylation site of Ser423:CS(p-S)VS
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Smad3 is a 50 kDa member of a family of proteins that act as key mediators of TGF beta superfamily signaling in cell proliferation, differentiation and development. The Smad family is divided into three subclasses: receptor regulated Smads, activin/TGF beta receptor regulated (Smad2 and 3) or BMP receptor regulated (Smad 1, 5, and 8); the common partner, (Smad4) that functions via its interaction to the various Smads; and the inhibitory Smads, (Smad6 and 7). Activated Smad3 oligomerizes with Smad4 upon TGF beta stimulation and translocates as a complex into the nucleus, allowing its binding to DNA and transcription factors. Phosphorylation of the two TGF beta dependent serines 423 and 425 in the C terminus of Smad3 is critical for Smad3 transcriptional activity and TGF beta signaling.
Size:50ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
Flow-Cyt(3μg/Test)
ICC(1:100)
Cross Reactive Species:Human
Mouse
Rat
Pig
僅供科研使用。不能用于人和動(dòng)物治療等其它臨床診斷用！
關(guān)鍵字： phospho-Smad3 (Ser42;Smad3 (phospho Ser42;p-Smad3 (Ser423);Smad3 (phospho S423);hSMAD3;HSPC193;Mothers against DPP ;SMAD3_HUMAN;
上海滬震實(shí)業(yè)有限公司是一家集研發(fā)、生產(chǎn)和銷售于一體的生命科學(xué)實(shí)驗(yàn)室產(chǎn)品生物科技企業(yè)。主營(yíng)產(chǎn)品：試劑盒，ELISA試劑盒，抗體，重組蛋白，血清，胎牛血清，細(xì)胞，原代細(xì)胞 細(xì)胞培養(yǎng)試劑，常用生化試劑。