Product Name：PKA Inhibitor Fragment (6-22) amide
CAS No.：121932-06-7
Synonyms：PKA抑制劑片段6-22
Catalog No.：198P36
Brand：HongTide（上海鴻肽生物）
Description：PKA Inhibitor Fragment (6-22) amide是一種 PKA 抑制劑多肽，是由17個氨基酸組成的直鏈多肽。在細胞生物學中，蛋白激酶 A (PKA) 是一個酶家族，其活性取決于細胞水平的環(huán) AMP (cAMP)。 PKA 也被認為是一種 cAMP 依賴性蛋白激酶。 PKA在細胞中具有多種功能，例如調節(jié)糖、糖原和脂質代謝，在體外能與 (Ala)Kemptide 相比，發(fā)現(xiàn) PKA 抑制劑片段 (6-22) 酰胺在抑制效力方面具有顯著差異，這可能是由于幾個非精氨酸殘基對這種效力差異的關鍵作用。以高效力抑制 CAMP 依賴性蛋白激酶的 PKA 抑制劑片段 (6-22) 的最小長度類似物是 17 個殘基 PKI-(6-22)-酰胺。 PKA 抑制劑片段 (6-22) 在活性位點的肽/蛋白質結合部分以低納摩爾 Ki 值的競爭方式相互作用。為了抑制 CAMP 依賴性蛋白激酶，PKA 抑制劑片段 (6-22) 需要假底物位點（殘基 14-22）和殘基 6-13 內的其他 NH2 末端決定簇。 PKA 抑制劑片段 (6-22) 可以清除與活性位點的結合區(qū)相互作用的模擬蛋白和肽底物，特別是在 COOH 末端假底物堿性域中。毫無疑問，PKA抑制劑片段（6-22）的整體親和力是由于氫鍵的數(shù)量以及與CAMP依賴性蛋白激酶活性位點的其他鍵相互作用。
PKA Inhibitor Fragment (6-22) amide is a PKA inhibitor peptide composed of 17 amino acids in a straight chain. In cellular biology, protein kinase A (PKA) is a family of enzymes whose activity depends on the cyclic AMP (cAMP) at the cellular level. PKA is also considered a cAMP dependent protein kinase. PKA has multiple functions in cells, such as regulating sugar, glycogen, and lipid metabolism. In vitro, compared with (Ala) Kemptide, the PKA inhibitor fragment (6-22) amide showed significant differences in inhibitory efficacy, which may be due to the key role of several non arginine residues in this difference in potency. The minimum length analog of the PKA inhibitor fragment (6-22) that inhibits CAMP dependent protein kinases with high potency is 17 residues of PKI - (6-22) - amide. The peptide/protein binding part of the PKA inhibitor fragment (6-22) at the active site interacts in a competitive manner with a low nanomolar Ki value. In order to inhibit CAMP dependent protein kinase, the PKA inhibitor fragment (6-22) requires a pseudo substrate site (residues 14-22) and other NH2 terminal determinants within residues 6-13. The PKA inhibitor fragment (6-22) can clear the mimic protein and peptide substrate interacting with the binding region of the active site, especially in the basic domain of the false substrate at the COOH end. There is no doubt that the overall affinity of the PKA inhibitor fragment (6-22) is due to the number of hydrogen bonds and the interaction with other bonds at the active site of the CAMP dependent protein kinase.
Purity (HPLC) ≥98.0%
Single impurity≤2%
Peptide content≥75.0%
Amino Acid Composition≤±15%
Bacterial Endotoxins≤50EU/mg
Solubility：H2O/DMSO
Vacuum-packed with aluminum foil bag or transparent bag.Various weights can be packed according to your requirements.
Storage:Cool dry place( Store at -20°C, away from the light)
Remark:For Research Use Only. Not for human use.
Sequence(1-letter code) | TYADFIASGRTGRRNAI-NH2 |
Sequence(3-letter code) | H-Thr-Tyr-Ala-Asp-Phe-Ile-Ala-Ser-Gly-Arg-Thr-Gly-Arg-Arg-Asn-Ala-Ile-NH2 |
Peptide Structure | 
|
Molecular Formula | C80H130N28O24 |
Molecular Weight | 1868.09 |
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關鍵字： 121932-06-7;PKA抑制劑片段6-22;PKA Inhibitor Fragme;多肽;多肽合成;
上海鴻肽生物科技有限公司（簡稱：鴻肽生物）是一家以多肽以及多肽上下游產品研發(fā)、生產、銷售以及技術轉讓為主要業(yè) 務科技公司。公司現(xiàn)有產品涵蓋：定制多肽、目錄多肽、氨基酸衍生物、小分子化合物；公司自成立以來，秉持“用質量作船， 以服務為帆，為科研服務”的企業(yè)理念，為國內外眾多科研工作者提供了優(yōu)質的服務及產品。