產(chǎn)品名稱
人APP 蛋白knockout HEK-293T cell line
Parental Cell Line
HEK293T
Organism
Human
Mutation description
Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 5
Passage number
<20
Knockout validation
Immunocytochemistry (ICC), Sanger Sequencing, Western Blot (WB)
經(jīng)測試應用
適用于: WB, ICCmore details
Biosafety level
2
常規(guī)說明
Recommended control: Human wild-type HEK293T cell line (ab255449). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
Cryopreservation cell medium: Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture medium: DMEM (High Glucose) + 10% FBS
Initial handling guidelines: Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method. Based on cell count, seed cells in an appropriate cell culture flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules.
4. Incubate the culture at 37°C incubator with 5% CO2. Cultures should be monitored daily.
Subculture guidelines:
All seeding densities should be based on cell counts gained by established methods.
A guide seeding density of 2x104 cells/cm2 is recommended.
A partial media change 24 hours prior to subculture may be helpful to encourage growth, if required.
Cells should be passaged when they have achieved 80-90% confluence.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
關鍵字： 淀粉樣前體蛋白;APP protein;APP重組蛋白;
湖北艾普蒂生物工程有限公司（簡稱：艾普蒂）由在國內(nèi)科研試劑領域有著十幾年從業(yè)經(jīng)驗的專業(yè)技術團隊和企業(yè)管理團隊組建而成，專門從事以抗體、蛋白、細胞、化學品為核心的試劑產(chǎn)品研發(fā)與銷售。
我們長期聚焦于生命科學、生物醫(yī)藥、醫(yī)療診斷、分析檢測等領域，專注于為生命科學和生物技術領域的客戶提供優(yōu)質(zhì)的產(chǎn)品和技術服務。提供生命科學 試劑、供應鏈服務等，為更多的生命科學科研工作者提供 專業(yè)、全面、安全、快捷的產(chǎn)品和服務。產(chǎn)品覆蓋 基因、蛋白、細胞、組織、動物等水平的各種研究。
致力于成長為我國重要的生命科學試劑和成果轉化一體化的領導者、致力于成為中國大型的科研試劑產(chǎn)品供應商之一。