中文名稱:分散酶II | 英文名稱:Dispase II |
CAS:42613-33-2 | 品牌: 碧云天 |
產地: 中國 | 保存條件: -20℃保存 |
純度規(guī)格: 詳詢 | 產品類別: 普通產品 |
貨號: ST2339 | 是否進口: 否 |
用途: 科研 | 產品規(guī)格: 詳詢 |
基本信息(General Information):
產品描述(Description):
Analysis Note | The enzyme can be initially dissolved in 50 mM Hepes/KOH pH 7.4, 150 mM NaCl at 10 mg/ml. Further dilutions should be made in the cell culture media most appropriate for the cells being dissociated. Dispase is a metalloproteinase and is activated by dival |
Application | The enzyme from Beyotime has been used in developing a protocol for ex vivo culture of mouse embryonic mammary buds. It has been used in the treatment of rat heart pieces during the isolation of mitochondria from rat heart. It has also been used for the iso |
Biochem/physiol Actions | Dispase II is a neutral protease that hydrolyzes the N-terminal peptide bonds of non-polar amino acid residues. It may be used for separating many tissues and cells grown in vitro. The enzyme is very gentle and does not damage cell membranes. |
Physical form | lyophilized powder containing calcium acetate and milk sugar |
Preparation Note | A 50 U/mL stock solution may be prepared by dissolving the powder in a buffer containing 10 mM NaAc (pH 7.5) and 5 mM CaAc. It should be stored at 4 °C. |
Unit Definition | One unit will hydrolyze casein to produce color equivalent to 1.0 mumole (181 mug) of tyrosine per min at pH 7.5 at 37°C (color by Folin-Ciocalteu reagent), unless otherwise indicated. |
性質(Properties):
activity | ≥0.5 units/mg solid |
solubility | 10 mM NaAc (pH 7.5) and 5 mM CaAc: soluble |
storage temp. | 2-8°C |
成立日期 | 2017-02-16 (8年) | 注冊資本 | 100.000000萬人民幣 |
員工人數(shù) | 50-100人 | 年營業(yè)額 | ¥ 500萬-1000萬 |
主營行業(yè) | 中間體,催化劑及助劑,生物化工,化學試劑,儀器耗材 | 經(jīng)營模式 | 貿易,工廠,試劑,定制,服務 |
產品名稱 | 價格 | 公司名稱 | 報價日期 | |
---|---|---|---|---|
詢價 |
廣東勁盛科技有限公司
|
2025-01-07 | ||
¥20 |
VIP4年
|
湖北科沃德化工有限公司
|
2025-01-08 | |
詢價 |
VIP1年
|
湖北鴻福達生物科技有限公司
|
2025-01-08 | |
¥80 |
VIP6年
|
河北創(chuàng)之源生物科技有限公司
|
2025-01-08 |