人氧化低密度脂蛋白(HUMAN OX-LDL)Quantity: 2.0 mg (milligrams) Protein/vial Concentration: 2.0 mg/ml(Protein) Packaging: Oxidized LDL is membrane filtered and aseptically packaged in a solutioncontaining phosphate-buffered saline at pH7.4 and <1 μM EDTA-Na2 Preparation: Human LDL (which was purified to homogeneity via Ultracentrifugation(1.019-1.063g/cc))is oxidized using 5μM Cu2SO4 (oxidant) in PBS at 37℃for 20 hours. Oxidation is terminated by adding excess EDTA- Na2. Each lotis analyzed gel electrophoresis for migration versus LDL. This lot ofOX-LDL migrates 2.0fold further than the native LDL. This type Oxidized LDLis widely used to investigate lipid metabolism, but not induce cell apoptosis.Apoptosis inducement choose High Oxidized LDL from our company. TBARS: TBARS is determined colorimetrically by using Malondialdehyde as a standard. Starting LDL 0.18 nmoles of MDA/mg ProteinOx-LDL 22.7 nmoles of MDA/mg ProteinStorage&Stability: This product is stable for 8 weeks after receipt when handled aseptically and stored at 2-8℃. NEVER FREEZE*Special Note After prolonged storage, some precipitate may be observed. This is normal for This product. Clarify out the aggregates by spinning in a microfuge for 2 minutes. Biological Sample lots of Oxidized LDL are evaluated for receptor binding toActivity: peritoneal macrophages in conjunction with our Di-lOx-LDL and [I-125]Ox-LDL *Preparations of Oxidized LDL are fairly unstable;plan your experiments in advance and use fresh material.FOR RESEARCH USE ONLY
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