中文名稱

【GSTP1】重組人谷胱甘肽S-轉(zhuǎn)移酶Pi1

英文名字

Recombinant Human Glutathione S-Transferase pi 1

供應(yīng)商

ProSpec

產(chǎn)品貨號(hào)

ENZ-427

背景資料

GSTP1 is a polymorphic gene encoding active， functionally different GSTP1 variant proteins that are believed to function in xenobiotic metabolism and have a part in susceptibility to cancer， and other diseases. GSTP1 is a glutathione S-transferase belonging to the pi class. GST family enzymes play a significant role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based upon the biochemical， immunologic and structural properties of the soluble GSTs they are grouped into 4 main classes: alpha， mu， pi， and theta. The GSTP1 enzyme acts by catalyzing the reaction of glutathione with an acceptor molecule to form a Sulfur-substituted glutathione. The reactions employing glutathione contribute the transformation of a broad range of electrophiles， including reactive products of lipid， protein， carcinogens， therapeutic drugs， environmental toxins， and products of oxidative stress.
The GSTP1 inactivation through CpG hypermethylation is frequent in pituitary adenomas and may be a factor in aggressive pituitary tumor behavior. GSTP1 is may be a transcriptional target of the p53 tumor suppressor gene. Single-nucleotide polymorphism in GSTP1 is linked to modified protein binding， which influence GSTP1’s contribution to carcinogen and drug metabolism， and possibly disease pathogenesis and/or drug response. GST-pi might have central roles in proliferation of androgen-independent human prostate cancer cells.

序列

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMPP YTVVYFPVRG RCAALRMLLA DQGQSWKEEV VTVETWQEGS LKASCLYGQL PKFQDGDLTL YQSNTILRHL GRTLGLYGKD QQEAALVDMV NDGVEDLRCK YISLIYTNYE AGKDDYVKAL PGQLKPFETL LSQNQGGKTF IVGDQISFAD YNLLDLLLIH EVLAPGCLDA FPLLSAYVGR LSARPKLKAF LASPEYVNLP INGNGKQ.

來(lái)源宿主

大腸桿菌中重組表達(dá)

溶解建議

提供的人谷胱甘肽S-轉(zhuǎn)移酶Pi1（GSTP1）產(chǎn)品為過(guò)濾滅菌的無(wú)色溶液；其中人谷胱甘肽S-轉(zhuǎn)移酶Pi1（GSTP1）產(chǎn)品溶解于20mM Tris-HCl緩沖液（pH 7.0)，30%甘油，1mM EDTA，0.1mM PMSF溶液中.

保存建議

人谷胱甘肽S-轉(zhuǎn)移酶Pi1（GSTP1）溶液可以在4℃冷藏保存4周；如果想延長(zhǎng)保存時(shí)間，建議分裝（加載體蛋白如0.1% HSA或 BSA）后置于-18℃凍存.請(qǐng)避免反復(fù)凍融.

其他

ProSpec 是一家全球著名細(xì)胞因子蛋白生產(chǎn)和研發(fā)的公司，具有20多年的生產(chǎn)經(jīng)驗(yàn)，專注于蛋白（重組及合成）生產(chǎn)研發(fā)。我們的核心產(chǎn)品包括：干擾素、白介素、骨蛋白、腫瘤壞死因子、成纖維生長(zhǎng)因子、瘦素、干細(xì)胞因子、催乳素、表皮生長(zhǎng)因子、胰島素樣生長(zhǎng)因子、神經(jīng)生長(zhǎng)因子、集落刺激因子、激素、酶、病毒抗原以及各種重組蛋白等。 領(lǐng)先的生產(chǎn)工藝和規(guī)模使其可以提供毫克到克級(jí)蛋白，價(jià)格優(yōu)于同類公司。絕大部分產(chǎn)品是天然成熟型蛋白，而不含有標(biāo)簽蛋白?？紤]到大部分研究者希望能更靈活地配制蛋白溶液，ProSpec-Tany絕大部分產(chǎn)品沒(méi)有添加保護(hù)劑或鹽，這就對(duì)工藝提出了更高的要求。同時(shí)，ProSpec-Tany絕大部分產(chǎn)品為凍干粉，因此易于運(yùn)輸和保存。