中文名稱

【GSTP1】重組人谷胱甘肽S-轉移酶Pi1

英文名字

Recombinant Human Glutathione S-Transferase pi 1

供應商

ProSpec

產品貨號

ENZ-427

背景資料

GSTP1 is a polymorphic gene encoding active， functionally different GSTP1 variant proteins that are believed to function in xenobiotic metabolism and have a part in susceptibility to cancer， and other diseases. GSTP1 is a glutathione S-transferase belonging to the pi class. GST family enzymes play a significant role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based upon the biochemical， immunologic and structural properties of the soluble GSTs they are grouped into 4 main classes: alpha， mu， pi， and theta. The GSTP1 enzyme acts by catalyzing the reaction of glutathione with an acceptor molecule to form a Sulfur-substituted glutathione. The reactions employing glutathione contribute the transformation of a broad range of electrophiles， including reactive products of lipid， protein， carcinogens， therapeutic drugs， environmental toxins， and products of oxidative stress.
The GSTP1 inactivation through CpG hypermethylation is frequent in pituitary adenomas and may be a factor in aggressive pituitary tumor behavior. GSTP1 is may be a transcriptional target of the p53 tumor suppressor gene. Single-nucleotide polymorphism in GSTP1 is linked to modified protein binding， which influence GSTP1’s contribution to carcinogen and drug metabolism， and possibly disease pathogenesis and/or drug response. GST-pi might have central roles in proliferation of androgen-independent human prostate cancer cells.

序列

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMPP YTVVYFPVRG RCAALRMLLA DQGQSWKEEV VTVETWQEGS LKASCLYGQL PKFQDGDLTL YQSNTILRHL GRTLGLYGKD QQEAALVDMV NDGVEDLRCK YISLIYTNYE AGKDDYVKAL PGQLKPFETL LSQNQGGKTF IVGDQISFAD YNLLDLLLIH EVLAPGCLDA FPLLSAYVGR LSARPKLKAF LASPEYVNLP INGNGKQ.

來源宿主

大腸桿菌中重組表達

溶解建議

提供的人谷胱甘肽S-轉移酶Pi1（GSTP1）產品為過濾滅菌的無色溶液；其中人谷胱甘肽S-轉移酶Pi1（GSTP1）產品溶解于20mM Tris-HCl緩沖液（pH 7.0)，30%甘油，1mM EDTA，0.1mM PMSF溶液中.

保存建議

人谷胱甘肽S-轉移酶Pi1（GSTP1）溶液可以在4℃冷藏保存4周；如果想延長保存時間，建議分裝（加載體蛋白如0.1% HSA或 BSA）后置于-18℃凍存.請避免反復凍融.

其他

ProSpec 是一家全球著名細胞因子蛋白生產和研發(fā)的公司，具有20多年的生產經驗，專注于蛋白（重組及合成）生產研發(fā)。我們的核心產品包括：干擾素、白介素、骨蛋白、腫瘤壞死因子、成纖維生長因子、瘦素、干細胞因子、催乳素、表皮生長因子、胰島素樣生長因子、神經生長因子、集落刺激因子、激素、酶、病毒抗原以及各種重組蛋白等。 領先的生產工藝和規(guī)模使其可以提供毫克到克級蛋白，價格優(yōu)于同類公司。絕大部分產品是天然成熟型蛋白，而不含有標簽蛋白?？紤]到大部分研究者希望能更靈活地配制蛋白溶液，ProSpec-Tany絕大部分產品沒有添加保護劑或鹽，這就對工藝提出了更高的要求。同時，ProSpec-Tany絕大部分產品為凍干粉，因此易于運輸和保存。