中文名稱

【GPBB】重組人糖原磷酸化酶

英文名字

Recombinant Human Glycogen Phosphorylase

供應(yīng)商

ProSpec

產(chǎn)品貨號

ENZ-282

背景資料

Glycogen phosphorylase is one of the phosphorylaseenzymes（EC2.4.1.1). It breaks up glycogeninto glucosesubunits. Glycogenis left with one less glucosemolecule， and the free glucosemolecule is in the form of glucose-1-phosphate. In order to be used for metabolism， it must be converted to glucose-6-phosphateby the enzyme phosphoglucomutase.
Glycogen phosphorylase can only act on linearchainsof glycogen（a 1-4 glycosidic linkage). Its work will immediately come to a halt four residues away from a 1-6 branch（which are exceedingly common in glycogen). In these situations， a debranching enzymeis necessary， which will straighten out the chain in that area. Additionally， an alpha 1-6 glucosidaseenzymeis required to break the remaining 1-6 residue that remains in the new linear chain. After all this is done， glycogen phosphorylase can continue.
An insulinstimulated enzyme known as phosphoprotein phosphatase（PP-1) inactivates glycogen phosphorylase to prevent glycogen break up.
GPBB - a sensitive marker for the AMI diagnosis within 4 hours after the onset of chest pain. It has also been shown that GPBB is increased in a considerable proportion of AMI patients within 2-3 hours from chest pain onset. GPBB is increased early in patients with unstable angina. GPBB can also be a sensitive marker for the detection of peri-operative myocardial ischaemia and infarction in patients undergoing coronary artery bypass grafting.

序列

該【GPBB】重組人糖原磷酸化酶Recombinant Human Glycogen Phosphorylase是Prospec品牌 產(chǎn)品，具體的蛋白序列可查看提供的英文版本產(chǎn)品說明書。

來源宿主

大腸桿菌中重組表達

溶解建議

提供的人糖原磷酸化酶（GPBB）產(chǎn)品為過濾滅菌的濃度為0.8 mg/ml無色溶液；其中每毫克人糖原磷酸化酶（GPBB）產(chǎn)品溶解于50%甘油溶液中.

保存建議

人糖原磷酸化酶（GPBB）溶液可以在10℃保存7天；如果想延長保存時間，建議分裝后置于-18℃凍存.請避免反復(fù)凍融.

其他

ProSpec 是一家全球著名細胞因子蛋白生產(chǎn)和研發(fā)的公司，具有20多年的生產(chǎn)經(jīng)驗，專注于蛋白（重組及合成）生產(chǎn)研發(fā)。我們的核心產(chǎn)品包括：干擾素、白介素、骨蛋白、腫瘤壞死因子、成纖維生長因子、瘦素、干細胞因子、催乳素、表皮生長因子、胰島素樣生長因子、神經(jīng)生長因子、集落刺激因子、激素、酶、病毒抗原以及各種重組蛋白等。 領(lǐng)先的生產(chǎn)工藝和規(guī)模使其可以提供毫克到克級蛋白，價格優(yōu)于同類公司。絕大部分產(chǎn)品是天然成熟型蛋白，而不含有標簽蛋白?？紤]到大部分研究者希望能更靈活地配制蛋白溶液，ProSpec-Tany絕大部分產(chǎn)品沒有添加保護劑或鹽，這就對工藝提出了更高的要求。同時，ProSpec-Tany絕大部分產(chǎn)品為凍干粉，因此易于運輸和保存。