中文名稱

DeepBlueC?；6-苯基-2,8-雙（苯甲基）-咪唑并[1,2-a]吡嗪-3（7H）-one

英文名字

DeepBlueC?; 6-phenyl-2,8-bis(phenylmethyl)-imidazo[1,2-a]pyrazin-3(7H)-one

供應(yīng)商

開曼生物

產(chǎn)品貨號

16157-1

背景資料

Coelenterazine is a luciferin, a light-emitting biomolecule that serves as a substrate for luciferases or as a constituent of photoproteins.{22804} The oxidation of coelenterazine to coelenteramide is accompanied by emission of blue light (emission maximum = 460-470 nm). Luciferase-mediated oxidation of coelenterazine or a derivative is used as an energy donor, typically to a form of green fluorescent protein (GFP), in bioluminescent resonance energy transfer (BRET) studies.{26295,26296,26290} Coelenterazine 400a is a bisdeoxy derivative of coelenterazine that produces an emission peak near 400 nm following conversion by Renilla luciferase (Rluc).{26297,26291} It is used in BRET2 protocols, whereas coelenterazine h, which has a 475 nm Rluc emission peak, is used in BRET1 protocols.{26296} The emission peak of coelenterazine 400a is commonly paired with class 1 and 3 GFPs, including GFP2 and GFP10.{26296,26291} BRET2 assays are commonly used in evaluating protein-protein interactions, including those involved in G protein-coupled receptor signaling.{26297,26291,26294}

產(chǎn)品描述

A bisdeoxy derivative of coelenterazine that produces an emission peak near 400 nm following conversion by Renilla luciferase; commonly used in BRET2 protocols to evaluate protein-protein interactions

保存建議

建議您在收到開曼生物品牌的Coelenterazine 400a (1 mg)產(chǎn)品后置于-20度保存，產(chǎn)品有效期為730天.

其他

開曼生物向全球科學(xué)工作者提供多研究領(lǐng)域的生化、免疫試劑和分析試劑盒，其產(chǎn)品被廣泛應(yīng)用于腫瘤、氧化氮、神經(jīng)學(xué)、凋亡、氧化性損傷、內(nèi)分泌學(xué)等不同研究領(lǐng)域.開曼生物可提供用于檢測的特色試劑盒，如：類花生酸類物質(zhì)、游離的生物標(biāo)志、環(huán)核苷、激素及氧化氮等；也提供多種高質(zhì)量試劑，包括：類花生酸類物質(zhì)、氧化氮試劑及許多相關(guān)脂質(zhì)、脂肪酸、酶和抗體