中文名稱

Actin 結(jié)合蛋白 Spin-Down 檢測試劑盒

英文名字

Actin Binding Protein Spin-Down Assay Kit

供應(yīng)商

Cytoskeleton

產(chǎn)品貨號

BK001

背景資料

該Actin 結(jié)合蛋白 Spin-Down 檢測試劑盒產(chǎn)品是Cytoskeleton品牌原裝進(jìn)口，Cytoskeleton品牌是細(xì)胞骨架研究專家，專注于生物化學(xué)和細(xì)胞過程研究中的純化蛋白和便捷試劑盒開發(fā)與生產(chǎn)。 

產(chǎn)品描述

收到該Actin 結(jié)合蛋白 Spin-Down 檢測試劑盒Actin Binding Protein Spin-Down Assay Kit產(chǎn)品后，查看廠家提供的產(chǎn)品說明書詳細(xì)介紹。

產(chǎn)品特點(diǎn)

The actin binding protein spin-down assay kit provides G- or F-actin plus positive (α-actinin ) and negative (Bovine Serum Albumin, BSA) binding control proteins. Actin binding occurs when there is an affinity for any site of actin. F-actin binding can be measured by using a spin down method. In this way centrifugation is used to separate F-actin from G-actin by differential sedimentation. F-actin binding proteins will co-sediment with actin filaments and form a pellet at the bottom of the centrifugation tube. Proteins with F -actin bundling activity can be detected since they will cause the F-actin to sediment at lower centrifugal forces than normal (14,000 x g vs 150,000 x g). F-actin severing proteins, G-actin binding proteins or non-actin binding proteins will stay in the supernatant. Severing proteins will be expected if more G-actin remains in the supernatant than in the negative control sample, and this activity should be tested further by measuring F-actin length distributions before and after adding the test protein. G-actin binding proteins can be measured by adding the test protein to G-actin and inducing polymerization, if the test protein sequesters G-actin then during the spin-down assay more actin will be left in the supernatant compared with the control. Actin can exist in two forms: Globular subunit (G-actin) and Filamentous polymer (F-actin) (See the About Actin page for more information). Both forms of actin interact with a plethora of proteins in the cell. To date there are over 50 distinct classes of Actin-Binding Proteins (ABPs), and the inventory is still far from complete. Actin Binding Proteins allow the actin cytoskeleton to respond rapidly to cellular and extracellular signals and are integral to cytoskeletal involvement in many cellular processes. These include cell shape and motility, muscle contraction, intracellular trafficking, cell pathogenesis and signal transduction. This kit contains skeletal muscle actin (Cat. # AKL99). The same kit is also available with non-muscle actin (Cat. # APHL99), see Cat. # BK013. The non-muscle actin spin-down kit may be more appropriate to use to study actin binding proteins from non-muscle tissues.

保存建議

廠家推薦常溫運(yùn)輸，收到產(chǎn)品后建議保存于4°C。

其他

Cytoskeleton公司成立于1993年，專注于生物化學(xué)和細(xì)胞過程研究中的純化蛋白和便捷試劑盒開發(fā)與生產(chǎn)。公司提供藥物篩選、信號轉(zhuǎn)導(dǎo)、蛋白質(zhì)轉(zhuǎn)錄后修飾（PTM）、細(xì)胞骨架研究相關(guān)的系列試劑盒和產(chǎn)品，尤其以細(xì)胞骨架相關(guān)研究見長，既能滿足于樣品較少的科學(xué)研究，也可以用于小規(guī)模篩選研究和高通量大規(guī)模篩選研究。此外，公司還提供微管蛋白，肌動蛋白，小G蛋白，GAPs，GEFs等現(xiàn)有產(chǎn)品的藥物篩選服務(wù)。