中文名稱

血漿/血清外泌體純化和RNA小提試劑盒（提取試劑盒）

英文名字

Plasma/Serum Exosome Purification and RNA Isolation Mini Kit

供應商

Norgen Biotek

產(chǎn)品貨號

NGB-58300

背景資料

血漿/血清外泌體純化和RNA小提試劑盒（提取試劑盒）產(chǎn)品的背景資料詳情查看Norgen Biotek品牌提供的產(chǎn)品說明書

產(chǎn)品描述

Norgen’s Plasma/Serum Exosome and Free-Circulating RNA Isolation Mini Kit constitutes an all-in-one system for the purification of exosomes and the sequential isolation of exosomal RNA and free-circulating RNA from different plasma/serum sample volumes ranging from 50 μL to 1 mL. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The kit is designed to isolate all sizes of extracellular vesicle RNA, including microRNA. The kit provides a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 μL to 100 μL. The RNA isolated from the purified exosomes is free from any protein-bound circulating RNA and is of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

產(chǎn)品特點

Purification and enrichment of intact plasma/serum exosomes for functional studies; Bind and elute all RNA irrespective of size or GC content, without bias; Isolate all sizes of exosomal and extracellular vesicle RNA, including microRNA; Versatile plasma/serum input volume (50 μL - 1 mL); No phenol extractions, Proteinase K treatment, nor carrier RNA required; No time-consuming ultracentrifugation, filtration nor special syringes required; No precipitation reagents, nor overnight incubation required; Compatible with plasma/serum from any species; Pure exosomes are purified and are free-from any other RNA-binding proteins.

保存建議

所有緩沖液應密封保存，并在室溫（15-25°C）下保存長達2年。

其他

Norgen Biotek成立于1998年，公司總部位于加拿大，是一家著名全球化生物技術公司，基于專利的碳化硅膜純化技術和無苯酚專利配方，生產(chǎn)高品質(zhì)的核酸和蛋白質(zhì)純化及富集產(chǎn)品用于研究和診斷。Norgen Biotek 在2003年獲得加拿大國家研究委員會頒發(fā)的科技創(chuàng)新獎，是一家致力于樣品制備并獲得ISO 9001（產(chǎn)品質(zhì)量）、ISO 13485（產(chǎn)品可用于商業(yè)化的醫(yī)療設備，包括用于體外診斷領域）和ISO 15189（可用于臨床檢測機分子診斷領域的研發(fā)能力）認證的生物科技公司。 目前該公司的產(chǎn)品多達700余種，提供一流的樣品制備試劑盒，應用于基因組和蛋白組等不同的領域。其產(chǎn)品主要覆蓋包括各種標本類型的DNA，RNA和蛋白質(zhì)的純化富集，從活檢樣本（包括尿液，血漿，血清和血液）中純化RNA和DNA，全系列的PCR試劑、緩沖液、分子量ladder和塑料過濾裝置。