背景資料

FemtoMax? Super Sensitive Chemiluminescent HRP Substrate is an extremely sensitive, nonradioactive, enhanced luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP).  FemtoMax? is designed for both western blotting and enzyme immunoassay (EIA) use.  FemtoMax? easily allows for the detection of femtogram (10-15) amounts of antigen using photographic film or other imaging methods, including highly sensitive CCD cameras.  Blots can be repeatedly exposed to X-ray film to obtain optimal results or stripped of detection reagents and re-probed. Use the same blotting conditions for FemtoMax? as you would when using Amersham ECL Plus? Substrate or Pierce SuperSignal? West Femto Substrate.

產(chǎn)品描述

Prepare FemtoMax? Super Sensitive Chemiluminescent HRP Substrate for use in microwell or membrane applications by mixing 1 mL of Luminol Reagent (Reagent A) with 1 mL of Reaction Buffer (Reagent B).  Mix well.  Protect from light.  Larger or smaller volumes of the substrate can be prepared by mixing components at the same 1:1 ratio.  FemtoMax? Super Sensitive Chemiluminescent HRP Substrate is a highly sensitive detection reagent.  Always carefully optimize all components of individual assays (antigens, antibodies, conjugates…) to minimize background reactivity associated with non-specific binding.

產(chǎn)品形式

Liquid - clear, colorless, odorless

保存建議

Store Chemiluminescent Substrate at 4° C prior to opening. Protect from moisture and light. No special shipping conditions or precautions are required.

其他

Rockland成立于1962年，是一家老牌抗體原產(chǎn)企業(yè)，其位于美國費城郊區(qū)的農(nóng)場保有近20種實驗動物。Rockland公司為生命科學領(lǐng)域提供豐富的、高質(zhì)量的抗體，包括載脂蛋白、細胞周期/凋亡、細胞因子、細胞信號蛋白、酶類、細胞外基質(zhì)、轉(zhuǎn)錄因子(NFkB)和泛素（Ubiquitin）等識別抗體，應(yīng)用范圍包括Western blot、免疫組化、免疫熒光和ELISA等。另外，Rockland也生產(chǎn)特別齊全的二抗，包括純化、生物素、HRP/ALP、熒光標記二抗，應(yīng)用十分廣泛。