IRDye 800CW Streptavidin
IRDye 800CW 鏈霉親和素
IRDye 800CW 鏈霉親和素以液體形式提供,緩沖液中含有 10 mM 磷酸鹽、183 mM NaCl、2.7 nM KCl,pH 7.4,疊氮化鈉 0.005% (w/v) 。
要使用,請在使用前短暫離心以消除可能在溶液中形成的聚集體。這將減少非特異性背景染色。對于大多數(shù)應用,0.2 到 1.0 μg/ml(1:1,000 到 1:5,000)的濃度通常是令人滿意的;但是,可能需要根據(jù)經驗確定適當?shù)南♂尪取?/span>
IRDye 800CW Streptavidin is supplied as a liquid in buffer containing 10 mM phosphate, 183 mM NaCl, 2.7 nM KCl, pH 7.4 with sodium azide 0.005% (w/v) as a preservative.
To use, centrifuge briefly before use to eliminate aggregates that may have formed in solution. This will reduce non-specific background staining. A final concentration of 0.2 to 1.0 μg/ml (1:1,000 to 1:5,000) is usually satisfactory for most applications; however, appropriate dilution may need to be determined empirically.
關鍵字: IRDye 800CW;鏈霉親和素;Streptavidin;
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