FibrOut?成纖維抑制劑 （原代細胞培養(yǎng)推薦★★★★★）
產(chǎn)品介紹
CHI Scientific研發(fā)的成纖維細胞生長抑制體系—FibrOut?，包含多種生化成分和有機試劑，能夠抑制成纖維細胞在原代細胞培養(yǎng)中的成長，進而促進靶細胞生長。每一種成纖維細胞抑制劑是組織和細胞特異性的，適用于相應的PrimaCellTM原代細胞培養(yǎng)體系，可以為您提供理想狀態(tài)的原代細胞。
規(guī)格型號
產(chǎn)品體系
人成纖維抑制劑
小鼠成纖維抑制劑
大鼠成纖維抑制劑
禽類成纖維抑制劑
質量檢測
每1ml FibrOut?可以混合于500ml培養(yǎng)基，每批試劑均經(jīng)過成纖維細胞抑制性能測試。
論文引用
1)[HTML] CD47 Promotes Age-Associated Deterioration in Angiogenesis, Blood Flow and Glucose Homeostasis.
Kedar Ghimire, * Yao Li, etc. al. Cells. 2020 Jul; 9(7): 1695
….. Mouse FibrOut 11 and Human FibrOut custom prepared for Blood Vessels and Endothelial Tissues were from Chi Scientific Inc. (Maynard,
MA, USA)….. Mouse FibrOut 11 and Human FibrOut custom prepared for blood vessels and endothelial tissues was added to the cell media following the manufacturers’ protocol to suppress fibroblast growth.
2)[HTML] Cancer/testis antigens expression during cultivation of melanoma and soft tissue sarcoma cells
A Danilova, V Misyurin, A Novik, D Girdyuk… - Clinical Sarcoma …, 2020 - Springer
… When cells reached sub-confluence, they were dispersed with 0.25% trypsin–0.02% EDTA
(Sigma-Aldrich, USA) and seeded in a new culture plate. Fibroblast growth-inhibitory cocktail
Human FibrOut? 9 (Chi Scientific Inc., USA) was used for fibroblast growth prevention …
3) Engineering a Bioartificial Human Colon Model Through Decellularization and Recellularization
HJ Chen, ML Shuler - Cancer Driver Genes, 2019 - Springer
… 5. Media with fibroblast inhibitory reagent: Colonic epithelial cell growth medium with 1%
fibroblast inhibitory reagent (Human Colon FibrOut? from CHI Scientific) for the first two to three
passages of primary cultures to prevent the outgrowth of fibroblasts. 6 …
4) A pumpless body-on-a-chip model using a primary culture of human intestinal cells and a 3D culture of liver cells
HJ Chen, P Miller, ML Shuler - Lab on a Chip, 2018 - pubs.rsc.org
… ng ml ?1 R-spondin 1 (R&D systems) and 50 ng ml ?1 Noggin (Peprotech, NJ) in collagen-I coated
flasks (BD scientific, NJ) and incubated in a humidified incubator at 5% CO 2 and 37 °C. After
48 hour culture, fibroblast inhibitory reagent (Human Colon FibrOut?, CHI Scientific …
5) [PDF] A novel way of harvesting a large population of endothelial cells for clinical and experimental use
AO Ilesanmi - Biomed Res Rev, 2018 - pdfs.semanticscholar.org
… If it is eventually decided that fibroblasts should be eliminated completely, there is a commercial
product, the Human Vascular Endothelial PrimaCell? system that includes an effective fibroblast
elimination system, Vascular Endothelial FibrOut? (www.chiscientific.com) …
6) [PDF] gene KIF23 in a mouse model of lung adenocarcinoma. Oncogene, 36 (1), pp. 110-121.(doi: 10.1038/onc. 2016.181) This is the author's final accepted version …
F Iltzsche - core.ac.uk
… ml fresh media. Tissue was broken up by pipetting and the cell suspension was cultured in media
supplemented with Mouse FibrOut? 9 (CHI Scientific). Cells were cultured until confluency was
reached, genotyped and stored for further processing. Antibodies …
7) Very long chain fatty acid β-oxidation in astrocytes: contribution of the ABCD1-dependent and-independent pathways
M Morita, S Shinbo, A Asahi… - Biological and …, 2012 - jstage.jst.go.jp
… The MessageMuter shRNAi Production kit was from EPICENTRE. FibrOut (System 4,
Mouse) was purchased from CHI Scientific (Maynard, MA, USA). Antimycin A and ro-
tenone were purchased from Sigma-Aldrich (St. Louis, MO, USA) …
8) Requirement of the NF-κB subunit p65/RelA for K-Ras–induced lung tumorigenesis
DS Bassères, A Ebbs, E Levantini, AS Baldwin - Cancer research, 2010 - AACR
9) p38α MAP kinase is essential in lung stem and progenitor cell proliferation and differentiation
JJ Ventura, S Tenbaum, E Perdiguero, M Huth… - Nature …, 2007 - nature.com
關鍵字： 成纖維細胞抑制;?FibrOut?;
齊氏生物科技有限公司成立于2009年，是一家專注于原代細胞培養(yǎng)和篩選、體外藥物代謝和藥物間相互作用評價產(chǎn)品的公司，為全球藥物公司、藥物研究所、疾控中心、新藥安評中心和各類科研機構提供符合標準規(guī)范產(chǎn)品，致力于打造原代細胞生物制藥研發(fā)和篩選平臺。