絲裂原激活蛋白激酶激酶4(MAP2K4)重組蛋白
[ PROPERTIES ]
Residues: Val137~Ser394
Tags: Two N-terminal Tags, His-tag and GST-tag
Accession: P45985
Host: E. coli
Subcellular Location: Cytoplasm. Nucleus.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris,
150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,
0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 5.9
Predicted Molecular Mass: 61.3kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8
oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
VDEK EQKQLLMDLD VVMRSSDCPY IVQFYGALFR EGDCWICMEL MSTSFDKFYK
YVYSVLDDVI PEEILGKITL ATVKALNHLK ENLKIIHRDI KPSNILLDRS GNIKLCDFGI
SGQLVDSIAK TRDAGCRPYM APERIDPSAS RQGYDVRSDV WSLGITLYEL ATGRFPYPKW
NSVFDQLTQV VKGDPPQLSN SEEREFSPSF INFVNLCLTK DESKRPKYKELLKHPFILMY
EERAVEVACY VCKILDQMPA TPSS
[ REFERENCES ]
1. Derijard B., et al. (1995) Science 267:682-685.
2. Lin A., et al. (1995) Science 268:286-290.
3. Su G.H., et al. (1998) Cancer Res. 58:2339-2342.
4. Tibbles L.A., et al. (1996) EMBO J. 15:7026-7035.
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關(guān)鍵字： JNKK;JNKK1;MAPKK4;絲裂原激活蛋白激酶激酶4(MAP2K4);
上海博爾森生物科技有限公司成立于2020年09月02日，注冊(cè)地位于上海市松江區(qū)石湖蕩鎮(zhèn)石湖新路95號(hào)。
上海博爾森生物科技有限公司雖然年輕，但朝氣蓬勃，志向遠(yuǎn)大； 更是一家專注于生命科學(xué)與生物技術(shù)領(lǐng)域，集產(chǎn)品研發(fā)、生產(chǎn)、銷售為一體的高科技公司，并立志以競(jìng)爭(zhēng)力的價(jià)格向客戶輸送高質(zhì)量的生物試劑。主要研究領(lǐng)域是生命科學(xué)，包括分子生物學(xué)、細(xì)胞生物學(xué)、免疫學(xué)試劑、重組蛋白，抗體，實(shí)驗(yàn)技術(shù)服務(wù)等多個(gè)應(yīng)用領(lǐng)域。主要產(chǎn)品有：Elisa檢測(cè)試劑盒、PCR檢測(cè)試劑盒、抗體、重組蛋白，蛋白定制，細(xì)胞系，原代細(xì)胞，常用實(shí)驗(yàn)試劑，染色液等科研產(chǎn)品。