脂質(zhì)運(yùn)載蛋白5(LCN5)重組蛋白
[ PROPERTIES ]
Residues: Gly22~Val177
Tags: Two N-terminal Tags, His-tag and GST-tag
Accession: P06911
Host: E. coli
Subcellular Location: Secreted.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris,
150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,
0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 6.0
Predicted Molecular Mass: 44.4kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of thetarget
protein. The loss rate was determined by accelerated thermal degradationtest,
that is, incubate the protein at 37oC for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
GAVVKDFDI SKFLGFWYEI AFASKMGTPG LAHKEEKMGA MVVELKENLL ALTTTYYSEDHCVLEKVTAT EGDGPAKFQV TRLSGKKEVV VEATDYLTYA IIDITSLVAGAVHRTMKLYSRSLDDNGEAL YNFRKITSDH GFSETDLYIL KHDLTCV
[ REFERENCES ]
1. Moore A., et al. (1990) Biol. Reprod. 43:497-506.
2. Girotti M., et al. (1992) Biochem. J. 281:203-210.
3. Brooks D.E., et al. (1986) J. Biol. Chem. 261:4956-4961.
4. Newcomer M.E., Ong D.E. (1990) J. Biol. Chem. 265:12876-12879.
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關(guān)鍵字： E-RABP;Mep10;LCN5;脂質(zhì)運(yùn)載蛋白5(LCN5)重組蛋白;
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