內(nèi)皮抑素(ES)重組蛋白
[ PROPERTIES ]
Residues: Ser1296~Gly1488 (Accession # F1LR02),
with two N-terminal Tags, His-tag and GST-tag. Host: E. coli
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Predicted isoelectric point: 7.8
Predicted Molecular Mass: 48.7kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of thetarget
protein. The loss rate was determined by accelerated thermal degradationtest,
that is, incubate the protein at 37oC for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, itssequence is listed below.
MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVLDIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALDVVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSDGSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEVLFQGPLGSEF -SYPTA RPWRADDILA NPPRLPDRQP YPGVPHHHHS HEHRPPAHPSPSPAHTHQDF HPVLHLVALN TPLSGGMRGI RGADFQCFQQ ARAVGLSGTF RAFLSSRLQDLYSIVRRADR SSVPIVNLKD EVLSPSWDTL FSGSQGQLHS GARIFSFDGR DVLRHPAWPQKSVWHGSDPS GRRLMESYCE TWRTEATG
[ REFERENCES ]
1.Gibbs R.A., et al. (2004) Nature 428:493-521.
2. Pufe T., et al. (2006) Ann. Anat. 187 (5-6): 461–72.
3. Tomono Y., et al. (2002) Cell Struct. Funct. 27 (1): 9–20.
4. Ergün S., et al. (2002) Angiogenesis 4 (3): 193–206.
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關(guān)鍵字： Recombinant;Endostatin;ES;內(nèi)皮抑素(ES)重組蛋白;
上海博爾森生物科技有限公司成立于2020年09月02日，注冊地位于上海市松江區(qū)石湖蕩鎮(zhèn)石湖新路95號。
上海博爾森生物科技有限公司雖然年輕，但朝氣蓬勃，志向遠(yuǎn)大； 更是一家專注于生命科學(xué)與生物技術(shù)領(lǐng)域，集產(chǎn)品研發(fā)、生產(chǎn)、銷售為一體的高科技公司，并立志以競爭力的價(jià)格向客戶輸送高質(zhì)量的生物試劑。主要研究領(lǐng)域是生命科學(xué)，包括分子生物學(xué)、細(xì)胞生物學(xué)、免疫學(xué)試劑、重組蛋白，抗體，實(shí)驗(yàn)技術(shù)服務(wù)等多個(gè)應(yīng)用領(lǐng)域。主要產(chǎn)品有：Elisa檢測試劑盒、PCR檢測試劑盒、抗體、重組蛋白，蛋白定制，細(xì)胞系，原代細(xì)胞，常用實(shí)驗(yàn)試劑，染色液等科研產(chǎn)品。