RNA核酸外切酶2同源物(REXO2)重組蛋白
[ PROPERTIES ]
Residues: Gln41~Asn213
Tags: N-terminal His-Tag
Accession: Q9Y3B8
Host: E. coli
Subcellular Location: Mitochondrion.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris,
150mM NaCl, pH8.0, containing 0.01% sarcosyl and 5%trehalose.
Predicted isoelectric point: 5.7
Predicted Molecular Mass: 21.7kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of thetarget
protein. The loss rate was determined by accelerated thermal degradationtest,
that is, incubate the protein at 37oC for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
QRMVWVDLEM TGLDIEKDQI IEMACLITDS DLNILAEGPN LIIKQPDELL DSMSDWCKEHHGKSGLTKAV KESTITLQQA EYEFLSFVRQ QTPPGLCPLA GNSVHEDKKF LDKYMPQFMKHLHYRIIDVS TVKELCRRWY PEEYEFAPKK AASHRALDDI SESIKELQFY RNN
[ REFERENCES ]
1. Lai C.-H., et al. (2000) Genome Res. 10:703-713.
2. Nguyen L.H., et al. (2000) J. Biol. Chem. 275:25900-25906.
3. Mechold U., et al. (2006) Nucleic Acids Res. 34:2364-2373.
4. Van Damme P., et al. (2012) Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454.
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關(guān)鍵字： SFN; RFN;SMFN;RNA核酸外切酶2同源物(REXO2)重;
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