嘌呤霉素敏感性氨肽酶(PSA)重組蛋白
[ PROPERTIES ]
Source: Prokaryotic expression. Host: E. coli
Residues: Gly584~Gln793
Tags: N-terminal His-Tag
Tissue Specificity: Liver, Kidney, Stomach. Subcellular Location: Cytoplasm, cytosol, Nucleus. Purity: >92%
Traits: Freeze-dried powder
Buffer formulation: 10mM PBS, pH7.4, containing 1mM DTT, 5%trehaloseand0.01% sarcosyl. Original Concentration: 200μg/mL
Applications: Positive Control; Immunogen; SDS-PAGE; WB. (May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 6.0
Predicted Molecular Mass: 24.7kDa
Accurate Molecular Mass: 25kDa as determined by SDS-PAGE reducingconditions.
[ USAGE ]
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Donot
vortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8
oC for one month. Aliquot and store at -80
oC for 12 months. Stability Test: The thermal stability is described by the loss rate. Thelossratewas determined by accelerated thermal degradation test, that is, incubatetheprotein at 37
oC for 48h, and no obvious degradation and precipitationwereobserved. The loss rate is less than 5% within the expiration dateunderappropriate storage condition.
[ SEQUENCE ]
[ IDENTIFICATION ]
Figure 1. SDS-PAGE
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關(guān)鍵字： NPEPPS;AAP-S;Cytosol;嘌呤霉素敏感性氨肽酶(PSA)重組蛋白;
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