碳酸酐酶Ⅻ(CA12)重組蛋白
[ PROPERTIES ]
Residues: Ala25~Leu302 (Accession # O43570),
with N-terminal His-Tag.
Host: E. coli
Subcellular Location: Membrane; Single-pass
type I membrane protein.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 1mM DTT, 5% trehalose, 0.05%
sarcosyl and preservative.
Predicted isoelectric point: 6.0
Predicted Molecular Mass: 32.8kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of thetarget
protein. The loss rate was determined by accelerated thermal degradationtest,
that is, incubate the protein at 37oC for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listedbelow.
MG HHHH HHSG SEF - APV NGS KW TY FGP DGE NSWSKKYP S C GG L LQSPI D LHSDILQYDAS LTPLEFQGYN LSANKQFLLT NNGHSVKLNL PSDMHIQGLQSRYSATQLHLHWGNPNDPHG SEHTVSGQHF AAELHIVHYN SDLYPDASTA SNKSEGLAVL AVLIEMGSFNPSYDKIFSHL QHVKYKGQEA FVPGFNIEEL LPERTAEYYR YRGSLTTPPC NPTVLWTVFRNPVQISQEQL LALETALYCT HMDDPSPREM INNFRQVQKF DERLVYTSFS QVQVCTAAGLSL
[ REFERENCES ]
1.Tuereci O., et al. (1998) Proc. Natl. Acad. Sci. U.S.A. 95:7608-7613.
2. Ivanov S.V., et al. (1998) Proc. Natl. Acad. Sci. U.S.A. 95:12596-12601.
3. Temperini C., et al. (2007) Bioorg. Med. Chem. Lett. 17:2210-2215.
4. Srivastava D.K., et al. (2007) J. Am. Chem. Soc. 129:5528-5537.
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關(guān)鍵字： CA-XII;CAXII; Carbonate;碳酸酐酶Ⅻ(CA12)重組蛋白;
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上海博爾森生物科技有限公司雖然年輕，但朝氣蓬勃，志向遠(yuǎn)大； 更是一家專注于生命科學(xué)與生物技術(shù)領(lǐng)域，集產(chǎn)品研發(fā)、生產(chǎn)、銷售為一體的高科技公司，并立志以競(jìng)爭(zhēng)力的價(jià)格向客戶輸送高質(zhì)量的生物試劑。主要研究領(lǐng)域是生命科學(xué)，包括分子生物學(xué)、細(xì)胞生物學(xué)、免疫學(xué)試劑、重組蛋白，抗體，實(shí)驗(yàn)技術(shù)服務(wù)等多個(gè)應(yīng)用領(lǐng)域。主要產(chǎn)品有：Elisa檢測(cè)試劑盒、PCR檢測(cè)試劑盒、抗體、重組蛋白，蛋白定制，細(xì)胞系，原代細(xì)胞，常用實(shí)驗(yàn)試劑，染色液等科研產(chǎn)品。