脯氨酰4-羥化酶α多肽Ⅱ(P4Ha2)重組蛋白
[ PROPERTIES ]
Residues: Ser207~Glu520
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: O15460
Host: E. coli
Subcellular Location: Endoplasmic reticulum
lumen.
Purity: >90%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 6.1
Predicted Molecular Mass: 39.9kDa
Accurate Molecular Mass: 43kDa as determined by SDS-PAGE reducingconditions.Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Note: The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins fromthe samegene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedtogive the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of thetarget
protein. The loss rate was determined by accelerated thermal degradationtest,
that is, incubate the protein at 37oC for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
SQVL DYLSYAVFQL GDLHRALELT RRLLSLDPSH ERAGGNLRYF EQLLEEEREKTLTNQTEAEL ATPEGIYERP VDYLPERDVY ESLCRGEGVK LTPRRQKRLF CRYHHGNRAPQLLIAPFKEE DEWDSPHIVR YYDVMSDEEI ERIKEIAKPK LARATVRDPK TGVLTVASYRVSKSSWLEED DDPVVARVNR RMQHITGLTV KTAELLQVAN YGVGGQYEPH FDFSRNDERDTFKHLGTGNR VATFLNYMSD VEAGGATVFP DLGAAIWPKK GTAVFWYNLL RSGEGDYRTRHAACPVLVGC KWVSNKWFHE
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關(guān)鍵字： Procollagen;Alpha ;P4Ha2;脯氨酰4-羥化酶α多肽Ⅱ(P4Ha2)重;
上海博爾森生物科技有限公司成立于2020年09月02日，注冊地位于上海市松江區(qū)石湖蕩鎮(zhèn)石湖新路95號。
上海博爾森生物科技有限公司雖然年輕，但朝氣蓬勃，志向遠(yuǎn)大； 更是一家專注于生命科學(xué)與生物技術(shù)領(lǐng)域，集產(chǎn)品研發(fā)、生產(chǎn)、銷售為一體的高科技公司，并立志以競爭力的價(jià)格向客戶輸送高質(zhì)量的生物試劑。主要研究領(lǐng)域是生命科學(xué)，包括分子生物學(xué)、細(xì)胞生物學(xué)、免疫學(xué)試劑、重組蛋白，抗體，實(shí)驗(yàn)技術(shù)服務(wù)等多個應(yīng)用領(lǐng)域。主要產(chǎn)品有：Elisa檢測試劑盒、PCR檢測試劑盒、抗體、重組蛋白，蛋白定制，細(xì)胞系，原代細(xì)胞，常用實(shí)驗(yàn)試劑，染色液等科研產(chǎn)品。