彈性蛋白酶3A(ELA3A)重組蛋白
[ PROPERTIES ]
Source: Prokaryotic expression. Host: E. coli
Residues: Ser16~His270
Tags: Two N-terminal Tags, His-tag and GST-tag
Tissue Specificity: Pancreas, Ovary. Purity: >95%
Traits: Freeze-dried powder
Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mMEDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. Original Concentration: 200μg/mL
Applications: Positive Control; Immunogen; SDS-PAGE; WB. (May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 6.2
Predicted Molecular Mass: 57.9kDa
Accurate Molecular Mass: 58kDa as determined by SDS-PAGE reducingconditions.
[ USAGE ]
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8
oC for one month. Aliquot and store at -80
oC for 12 months. Stability Test: The thermal stability is described by the loss rate. Thelossratewas determined by accelerated thermal degradation test, that is, incubatetheprotein at 37
oC for 48h, and no obvious degradation and precipitationwereobserved. The loss rate is less than 5% within the expiration dateunderappropriate storage condition.
[ SEQUENCE ]
[ IDENTIFICATION ]
Figure 1. SDS-PAGE
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關(guān)鍵字： CELA3A;ELA3; Chymotrypsin;彈性蛋白酶3A(ELA3A)重組蛋白;
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