中文名稱(chēng)
【AKR1B1】重組人醛糖還原酶
英文名字
Recombinant Human Aldose Reductase
供應(yīng)商
ProSpec
產(chǎn)品貨號(hào)
ENZ-390
產(chǎn)品報(bào)價(jià)
¥800/10ug ¥2080/50ug ¥28980/1mg
產(chǎn)品說(shuō)明書(shū)
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產(chǎn)品新聞
背景資料
AKR1B1 is part of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. AKR1B1 catalyzes the reduction several aldehydes, including the aldehyde form of glucose, and thus involved in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. AKR1B1 catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. Transgenic mice over expressing human aldose reductase show that AKR1B1 is a key player in ischemic injury and impairment of functional and metabolic recovery after ischemia. Aldose Reductase is an obligatory mediator of TNF-alpha signaling leading to an increase in the expression of adhesion molecules and increased binding of monocytes to the endothelium. AKR1B1 is a critical regulator of TNF-alpha-induced apoptotic signaling in endothelial cells.
序列
MASRLLLNNG AKMPILGLGT WKSPPGQVTE AVKVAIDVGY RHIDCAHVYQ NENEVGVAIQ EKLREQVVKR EELFIVSKLW CTYHEKGLVK GACQKTLSDL KLDYLDLYLI HWPTGFKPGK EFFPLDESGN VVPSDTNILD TWAAMEELVD EGLVKAIGIS NFNHLQVEMI LNKPGLKYKP AVNQIECHPY LTQEKLIQYC QSKGIVVTAY SPLGSPDRPW AKPEDPSLLE DPRIKAIAAK HNKTTAQVLI RFPMQRNLVV IPKSVTPERI AENFKVFDFE LSSQDMTTLL SYNRNWRVCA LLSCTSHKDY PFHEEF.
來(lái)源宿主
大腸桿菌中重組表達(dá)
溶解建議
提供的人醛糖還原酶(AKR1B1)產(chǎn)品為過(guò)濾滅菌的無(wú)色溶液;其中人醛糖還原酶(AKR1B1)產(chǎn)品溶解于20mM Tris-HCl緩沖液(pH 8), 10%甘油和1mM DTT溶液中.
保存建議
人醛糖還原酶(AKR1B1)溶液可以在4℃冷藏保存2-4周;如果想延長(zhǎng)保存時(shí)間,建議分裝(加載體蛋白如0.1% HSA或 BSA)后置于-20℃凍存.請(qǐng)避免反復(fù)凍融.
其他
ProSpec 是一家全球著名細(xì)胞因子蛋白生產(chǎn)和研發(fā)的公司,具有20多年的生產(chǎn)經(jīng)驗(yàn),專(zhuān)注于蛋白(重組及合成)生產(chǎn)研發(fā)。我們的核心產(chǎn)品包括:干擾素、白介素、骨蛋白、腫瘤壞死因子、成纖維生長(zhǎng)因子、瘦素、干細(xì)胞因子、催乳素、表皮生長(zhǎng)因子、胰島素樣生長(zhǎng)因子、神經(jīng)生長(zhǎng)因子、集落刺激因子、激素、酶、病毒抗原以及各種重組蛋白等。 領(lǐng)先的生產(chǎn)工藝和規(guī)模使其可以提供毫克到克級(jí)蛋白,價(jià)格優(yōu)于同類(lèi)公司。絕大部分產(chǎn)品是天然成熟型蛋白,而不含有標(biāo)簽蛋白??紤]到大部分研究者希望能更靈活地配制蛋白溶液,ProSpec-Tany絕大部分產(chǎn)品沒(méi)有添加保護(hù)劑或鹽,這就對(duì)工藝提出了更高的要求。同時(shí),ProSpec-Tany絕大部分產(chǎn)品為凍干粉,因此易于運(yùn)輸和保存。
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