腫瘤壞死因子受體超家族成員1A(TNFR1)重組蛋白
[ PROPERTIES ]
Residues: Thr60~Tyr236 (Accession # P19438),
with two N-terminal Tags, His-tag and GST-tag. Host: E. coli
Subcellular Location: Cell membrane;
Single-pass type I membrane protein. Golgi
apparatus membrane; Secreted.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 6.4
Predicted Molecular Mass: 51.9kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its
sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID
GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV
DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK
KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR
GSTAIGMK ET AAAKFE RQ HM DSP D LGT LE V LFQ GP LGS EF -T KCHK GTY LY N
DCP GP G Q D T D CRE CE S GS F T AS E N H L R HC L SCS K C RK EMG QV EI SS C T V D
RD TV C G CRK N QY RHYW SE N L FQC F N CS L C L NG TV H LS CQE KQ NT V C T CHA
GFFLRENECV SCSNCKKSLE CTKLCLPQIE NVKGTEDSGT TVLLPLVIFF GLCLLSLLFI
GLMYRY
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關鍵字： CD120A;P55;TNFRSF1A;腫瘤壞死因子受體超家族成員1A(TNFR;
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