纖溶酶原(Plg)重組蛋白
[ PROPERTIES ]
Residues: Gln274~Cys560 (Accession # P00747),
with N-terminal His-Tag.
Host: E. coli
Subcellular Location: Secreted.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Predicted isoelectric point: 6.7
Predicted Molecular Mass: 33.5kDa
Accurate Molecular Mass: 38kDa as determined by SDS-PAGE reducing conditions.
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Note: The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to
give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF -QCLKGTG ENY RGNVAVT VSGHTCQHWS AQTP HTHNRT
PENFPCKNLD ENYCRNPDGK RAPWCHTTNS QVRWEYCKIP SCDSSPVSTE QLAPTAPPEL
TPVVQDCY HG DGQSY RGTSS TTT TGKK CQS WSSM TP HRHQ KTPENYP NAG
LTMNYCRNPD ADKGPWCFTT DPSVRWEYCN LKKCSGTEAS VVAPPPVVLL PDVETPSEED
CM FG N GK GY R GK RAT T V TG T PC Q DWAA QE P HR HSI F TP E T NP RA G L EK NY
CRNPDGDVGG PWCYTTNPRK LYDYCDVPQC
[ REFERENCES ]
1.Forsgren M., et al. (1987) FEBS Lett. 213:254-260.
2. Malinowski D.P., et al. (1984) Biochemistry 23:4243-4250.
3. Wiman B., Wallen P. (1975) Eur. J. Biochem. 58:539-547.
4. Trexler M., et al. (1982) J. Biol. Chem. 257:7401-7406.
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關(guān)鍵字： PL;Plasmin;Activation;纖溶酶原(Plg)重組蛋白;
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