血管性血友病因子(vWF)重組蛋白
[ PROPERTIES ]
Source: Prokaryotic expression. Host: E. coli
Residues: Asp1498~Val1665
Tags: N-terminal His-Tag
Tissue Specificity: Blood. Subcellular Location: Secreted, extracellular space, extracellular matrix. Purity: >92%
Traits: Freeze-dried powder
Buffer formulation: PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01%
sarcosyl and Proclin300. Original Concentration: 200ug/mL
Applications: SDS-PAGE; WB; ELISA; IP; CoIP; ReporterAssays; Purification;
Amine Reactive Labeling. (May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 5.0
Predicted Molecular Mass: 20.4kDa
Accurate Molecular Mass: 18kDa as determined by SDS-PAGE reducing conditions.
[ USAGE ]
Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8
oC for one month. Aliquot and store at -80
oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate
was determined by accelerated thermal degradation test, that is, incubate the
protein at 37
oC for 48h, and no obvious degradation and precipitation were
observed.The loss rate is less than 5% within the expiration date under
appropriate storage condition.
[ SEQUENCE ]
[ IDENTIFICATION ]
Figure 1. SDS-PAGE
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關(guān)鍵字： F8VWF;VWD;von;血管性血友病因子(vWF)重組蛋白;
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