心肌營養(yǎng)素1(CT1)重組蛋白
[ DESCRIPTION DESCRIPTION DESCRIPTION DESCRIPTION ]
Protein Protein Protein Protein Names: Cardiotrophin 1
Synonyms: Synonyms: Synonyms: Synonyms: CT1
Size: 100 μg
Source: Source: Source: Source: Escherichia coli-derived
Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Secreted.
[ PROPERTIES ROPERTIES ROPERTIES ROPERTIES ]
Residues Residues Residues Residues: Met1~Ala201 (Accession # Q16619), with one N-terminal His-tag.
Grade & Purity: Purity: Purity: Purity: >95%, 22.7 kDa as determined by SDS-PAGE reducing conditions.
Formulation: Formulation: Formulation: Formulation: Supplied as liquid form in Phosphate buffered saline(PBS), pH 7.4.
Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0 EU per 1μg (determined by the LAL method).
Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 22.7 kDa
Predicted Predicted Predicted Predicted isoelectric isoelectric isoelectric isoelectric point: 8.7
[ PREPARATION PREPARATION PREPARATION PREPARATION ]
Reconstitute in PBS, PH7.2-PH7.4.
[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]
Storage: Storage: Storage: Storage: Avoid repeated repeated repeated repeated freeze/thaw freeze/thaw freeze/thaw freeze/thaw cycles. cycles. cycles. cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months .
Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was
determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious
degradation and precipitation were observed. (Referring from China Biological Products Standard, which was
calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under
appropriate storage condition.
[ BACKGROUND BACKGROUND BACKGROUND BACKGROUND]
The target protein is fused with one N-terminal His-tag. Their sequence is listed below.
MGHHHHHHSGSEF MSRREGSLED PQTDSSVSLL PHLEAKIRQT HSLAHLLTKY AEQLLQEYVQ LQGDPFGLPS FSPPRLPVAG
LSAPAPSHAG LPVHERLRLD AAALAALPPL LDAVCRRQAE LNPRAPRLLR RLEDAARQAR ALGAAVEALL AALGAANRGP
RAEPPAATAS AASATGVFPA KVLGLRVCGL YREWLSRTEG DLGQLLPGGS A
[ REFERENCES REFERENCES REFERENCES REFERENCES ]
1. Pennica D., et al. (1996) Cytokine. 8(3):183-9.
2. Jougasaki M. (2010) Adv Clin Chem. 52:41-76.
3. Latchman DS. (2000) Pharmacol Ther. 85(1):29-37.
4. Railson JE., et al. (2002) Cytokine. 17(5):243-53.
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關(guān)鍵字： CTF1;Cardiotrophin;CT1;心肌營養(yǎng)素1(CT1)重組蛋白;
上海博爾森生物科技有限公司成立于2020年09月02日，注冊地位于上海市松江區(qū)石湖蕩鎮(zhèn)石湖新路95號。
上海博爾森生物科技有限公司雖然年輕，但朝氣蓬勃，志向遠大； 更是一家專注于生命科學(xué)與生物技術(shù)領(lǐng)域，集產(chǎn)品研發(fā)、生產(chǎn)、銷售為一體的高科技公司，并立志以競爭力的價格向客戶輸送高質(zhì)量的生物試劑。主要研究領(lǐng)域是生命科學(xué)，包括分子生物學(xué)、細胞生物學(xué)、免疫學(xué)試劑、重組蛋白，抗體，實驗技術(shù)服務(wù)等多個應(yīng)用領(lǐng)域。主要產(chǎn)品有：Elisa檢測試劑盒、PCR檢測試劑盒、抗體、重組蛋白，蛋白定制，細胞系，原代細胞，常用實驗試劑，染色液等科研產(chǎn)品。