巨噬細胞移動抑制因子(MIF)重組蛋白
[ PROPERTIES ]
Residues: Met3~Asn111
Tags: N-terminal His-Tag
Accession: P14174
Host: E. coli
Subcellular Location: Secreted. Cytoplasm. Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 1mM DTT, 5% trehalose, 0.05%
sarcosyl and preservative.
Predicted isoelectric point: 7.1
Predicted Molecular Mass: 13.4kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
MFIVNTNV PRASVPDGFL SELTQQLAQA TGKPPQYIAV HVVPDQLMAF GGSSEPCALC
SLHSIGKIGG AQNRSYSKLL CGLLAERLRI SPDRVYINYY DMNAANVGWN N
[ REFERENCES ]
1. Kleemann R., et al. (2000) Nature 408:211-216.
2. Wistow G.J., et al. (1993) Proc. Natl. Acad. Sci. U.S.A. 90:1272-1275.
3. Gevaert K., et al. (2003) Nat. Biotechnol. 21:566-569.
4. Mikayama T., et al. (1993) Proc. Natl. Acad. Sci. U.S.A. 90:10056-10060.
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關鍵字： GIF;GLIF;MMIF;巨噬細胞移動抑制因子(MIF)重組蛋白;
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