細(xì)胞周期素D2(CCND2)重組蛋白
[ PROPERTIES ]
Residues: Glu68~Ala278
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: P30279
Host: E. coli
Subcellular Location: Nucleus. Cytoplasm.
Membrane.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris,
150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,
0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 5.6
Predicted Molecular Mass: 27.5kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
EEQ KCEEEVFPLA MNYLDRFLAG VPTPKSHLQL LGAVCMFLAS KLKETSPLTA
EKLCIYTDNS IKPQELLEWE LVVLGKLKWN LAAVTPHDFI EHILRKLPQQ REKLSLIRKH
AQTFIALCAT DFKFAMYPPS MIATGSVGAA ICGLQQDEEV SSLTCDALTE LLAKITNTDV
DCLKACQEQI EAVLLNSLQQ YRQDQRDGSK SEDELDQA
[ REFERENCES ]
Xiong Y., et al. (1992) Genomics 13:575-584.
2. Palmero I., et al. (1993) Oncogene 8:1049-1054.
3. Inaba T., et al. (1992) Genomics 13:565-574.
4. Wang Z., et al. (2008) Cell Struct. Funct. 33:171-183.
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關(guān)鍵字： CCN-D2;G1/S-Specific;CCND2;細(xì)胞周期素D2(CCND2)重組蛋白;
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