維甲酸誘導基因1蛋白(DDX58)重組蛋白
[ PROPERTIES ]
Source: Prokaryotic expression. Host: E. coli
Residues: Asp550~Phe776
Tags: N-terminal His-Tag
Tissue Specificity: Liver, Brain, Spleen, Stomach, Lung. Subcellular Location: Ruffle membrane. Cytoplasm, cytoskeleton. Cell junction,
tight junction. Purity: >98%
Endotoxin Level: <1.0EU per 1μg (determined by the LAL method). Traits: Freeze-dried powder
Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. Original Concentration: 200ug/mL
Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Reporter Assays; Purification;
Amine Reactive Labeling. (May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 5.9
Predicted Molecular Mass: 29.5kDa
Accurate Molecular Mass: 30kDa as determined by SDS-PAGE reducing conditions.
[ USAGE ]
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0
mg/mL. Do not vortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8
oC for one month. Aliquot and store at -80
oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate
was determined by accelerated thermal degradation test, that is, incubate the
protein at 37
oC for 48h, and no obvious degradation and precipitation were
observed.The loss rate is less than 5% within the expiration date under
appropriate storage condition.
[ SEQUENCE ]
[ IDENTIFICATION ]
Figure 1. SDS-PAGE
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關鍵字： RIG-I;RIGI;DEAD;維甲酸誘導基因1蛋白(DDX58)重組蛋;
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