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CAS RN: 28718-90-3 | 產(chǎn)品編碼: D5888
DAPI 2HCl (0.2mL×5) (1mg/mL in Water) [for Cell Staining]
純度/分析方法:
別名:
- DAPI 2HCl (0.2mL×5) (1mg/mL于水中) [用于細(xì)胞染色]
- 4',6-二脒基-2-苯基吲哚二鹽酸鹽 (0.2mL×5) (1mg/mL于水中)
- 2-(4-氨基甲?;交?-1H-吲哚-6-羧酰胺二鹽酸鹽 (0.2mL×5) (1mg/mL于水中)
- 6-脒基-2-(4-脒基苯基)吲哚二鹽酸鹽 (0.2mL×5) (1mg/mL于水中)
- 4',6-Diamidino-2-phenylindole Dihydrochloride (0.2mL×5) (1mg/mL in Water)
- 2-(4-Carbamimidoylphenyl)-1H-indole-6-carboximidamide Dihydrochloride (0.2mL×5) (1mg/mL in Water)
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產(chǎn)品文檔:
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補(bǔ)充產(chǎn)品信息:
Product Information
- Volume : 0.2 mL x 5 vial
- Concentration : 1.0 mg/mL
Description
- The DAPI is a fluorescent stain for labeling DNA.
DNA-bound DAPI is readily excited with UV light and emits fluorescence in the blue region of the visible light spectrum (Excitation : 358 nm / Emission : 461 nm).
Description for Use
- Fluorescence Staining of Cell Nucleus
- Fix and permeabilize cells as desired.
- Dilute DAPI solution 1/1000 (*) with PBS or an appropriate buffer.
*Typical working concentration used in most applications is 0.1 μg/mL - 10 μg/mL. - Incubate with cells at room temperature for 5-20 minutes (**).
**Extend Incubate time as desired. - Mount sample with an appropriate medium and proceed to imaging.
Storage
- Store at -20 °C for long term storage. It may be kept at 4 °C for short term storage. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Related Products
- A2412 DAPI·2HCl [for Biochemical Research]
- B6236 Bisbenzimide H 33258 (1mg/mL in Water) (0.2mL*5) [for Cell Staining]
- E1363 Ethidium Bromide (0.5mg/mL in Water) (in Dropper Bottle) [for Electrophoresis]
- G0406 Goat Anti-Mouse IgG FITC Conjugate
- G0452 Goat Anti-Rabbit IgG FITC Conjugate
- G0453 Goat Anti-Mouse IgM FITC Conjugate
- G0569 Goat Anti-Mouse IgG R-PE Conjugate
- G0577 Goat Anti-Rabbit IgG R-PE Conjugate
- H1343 Bisbenzimide H 33258 Hydrate [for Biochemical Research]
- S0966 Streptavidin FITC Conjugate
- T3885 Streptavidin R-PE Conjugate
技術(shù)規(guī)格
Appearance | Light yellow to Yellow clear liquid |
DNA staining test | pass test |
物性(參考值)
最大吸收波長(zhǎng) | 342(H2O) nm |
GHS
相關(guān)法規(guī)
RTECS# | NL5995054 |
運(yùn)輸信息
監(jiān)管條件代碼(*) |
應(yīng)用
Fluorescent staining of DNA in cells
References
- Binding of 4',6-diamidino-2-phenylindole (DAPI) to AT Regions of DNA: Evidence for an Allosteric Conformational Change
- Photoconversion of DAPI and Hoechst dyes to green and red emitting forms after exposure to UV excitation
- Protein kinase C involvement in aloe-emodin- and emodin-induced apoptosis in lung carcinoma cell
- Prostaglandin EP2 receptor downstream of Notch signaling inhibits differentiation of human skeletal muscle progenitors in differentiation conditions
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