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CAS RN: 28718-90-3 | 產(chǎn)品編碼: D5888
DAPI 2HCl (0.2mL×5) (1mg/mL in Water) [for Cell Staining]
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![DAPI 2HCl (0.2mL×5) (1mg/mL in Water) [for Cell Staining]](/_ui/responsive/theme-tci/images/missing_product_zoom.jpg "DAPI 2HCl (0.2mL×5) (1mg/mL in Water) [for Cell Staining]")
純度/分析方法:
別名：
- DAPI 2HCl (0.2mL×5) (1mg/mL于水中) [用于細胞染色]
- 4',6-二脒基-2-苯基吲哚二鹽酸鹽 (0.2mL×5) (1mg/mL于水中)
- 2-(4-氨基甲?；交?-1H-吲哚-6-羧酰胺二鹽酸鹽 (0.2mL×5) (1mg/mL于水中)
- 6-脒基-2-(4-脒基苯基)吲哚二鹽酸鹽 (0.2mL×5) (1mg/mL于水中)
- 4',6-Diamidino-2-phenylindole Dihydrochloride (0.2mL×5) (1mg/mL in Water)
- 2-(4-Carbamimidoylphenyl)-1H-indole-6-carboximidamide Dihydrochloride (0.2mL×5) (1mg/mL in Water)
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產(chǎn)品文檔：
| 規(guī)格 | 單價 | 上海 | 天津 | 日本* | 數(shù)量 |
庫存詳情
|
|---|---|---|---|---|---|---|
| 1SET |
￥380.00
|
2 | 1 | 10 |
|
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* 無具體發(fā)貨日期的情況，如：顯示“8個工作日后發(fā)貨”，將在您訂購日起的8個工作日后發(fā)貨。
* 我們將以最優(yōu)方式從上海/天津兩大倉庫發(fā)貨。國內(nèi)庫存不足，需兩周左右向日本總部調(diào)貨。
* 對于可分裝產(chǎn)品，11:30前的訂單，當天發(fā)貨；11:30后的訂單，隔天發(fā)貨。
* 如需大包裝，請點擊“大包裝詢價”按鈕（對于某些產(chǎn)品我們無法提供大包裝）。
* TCI會經(jīng)常復(fù)審儲藏條件以對其進行優(yōu)化，請以在線目錄為準，敬請留意。
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補充產(chǎn)品信息:
Product Information
- Volume : 0.2 mL x 5 vial
- Concentration : 1.0 mg/mL
Description
- The DAPI is a fluorescent stain for labeling DNA.
DNA-bound DAPI is readily excited with UV light and emits fluorescence in the blue region of the visible light spectrum (Excitation : 358 nm / Emission : 461 nm).
Description for Use
- Fluorescence Staining of Cell Nucleus
- Fix and permeabilize cells as desired.
- Dilute DAPI solution 1/1000 (*) with PBS or an appropriate buffer.
*Typical working concentration used in most applications is 0.1 μg/mL - 10 μg/mL. - Incubate with cells at room temperature for 5-20 minutes (**).
**Extend Incubate time as desired. - Mount sample with an appropriate medium and proceed to imaging.
Storage
- Store at -20 °C for long term storage. It may be kept at 4 °C for short term storage. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Related Products
- A2412 DAPI·2HCl [for Biochemical Research]
- B6236 Bisbenzimide H 33258 (1mg/mL in Water) (0.2mL*5) [for Cell Staining]
- E1363 Ethidium Bromide (0.5mg/mL in Water) (in Dropper Bottle) [for Electrophoresis]
- G0406 Goat Anti-Mouse IgG FITC Conjugate
- G0452 Goat Anti-Rabbit IgG FITC Conjugate
- G0453 Goat Anti-Mouse IgM FITC Conjugate
- G0569 Goat Anti-Mouse IgG R-PE Conjugate
- G0577 Goat Anti-Rabbit IgG R-PE Conjugate
- H1343 Bisbenzimide H 33258 Hydrate [for Biochemical Research]
- S0966 Streptavidin FITC Conjugate
- T3885 Streptavidin R-PE Conjugate
技術(shù)規(guī)格
| Appearance | Light yellow to Yellow clear liquid |
| DNA staining test | pass test |
物性（參考值）
| 最大吸收波長 | 342 nm (H__2O) |
GHS
相關(guān)法規(guī)
| RTECS# | NL5995054 |
運輸信息
| 監(jiān)管條件代碼(*) |
應(yīng)用
Fluorescent staining of DNA in cells
References
- Binding of 4',6-diamidino-2-phenylindole (DAPI) to AT Regions of DNA: Evidence for an Allosteric Conformational Change
- Photoconversion of DAPI and Hoechst dyes to green and red emitting forms after exposure to UV excitation
- Protein kinase C involvement in aloe-emodin- and emodin-induced apoptosis in lung carcinoma cell
- Prostaglandin EP2 receptor downstream of Notch signaling inhibits differentiation of human skeletal muscle progenitors in differentiation conditions
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