3-MA (3-Methyladenine)

別名: NSC 66389 中文名稱(chēng)：3-甲基腺嘌呤

目錄號(hào)：S2767 Purity: 99.97%

3-MA (3-Methyladenine)是一種選擇性PI3K抑制劑，作用于Vps34和PI3Kγ，在 HeLa細(xì)胞中IC50分別為25 μM和60 μM；永久性抑制I型PI3K，但對(duì)III型PI3K的抑制是短暫的，也會(huì)阻斷自噬體的形成。在溶液中不穩(wěn)定，請(qǐng)現(xiàn)配現(xiàn)用！

3-MA (3-Methyladenine) Chemical Structure

CAS: 5142-23-4

規(guī)格	價(jià)格	庫(kù)存
50mg	579.66	現(xiàn)貨
500mg	3010.37	現(xiàn)貨
2g	6470.1	現(xiàn)貨
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產(chǎn)品質(zhì)控

批次：純度： 99.97%

99.97

常與3-MA (3-Methyladenine)一起在實(shí)驗(yàn)中被使用的化合物

Chloroquine

3-Methyladenine和Chloroquine可阻斷MCF-7和HFF細(xì)胞中tetrandrine誘導(dǎo)的自噬。

Wang H, et al. Cell Biosci. 2015 Jan 14;5:4.

Rapamycin

3-Methyladenine顯著抑制LPS處理小鼠的結(jié)腸組織自噬，而Rapamycin則誘導(dǎo)自噬。

Zhou M, et al. EBioMedicine. 2018 Sep;35:345-360.

Necrostatin-1

3-Methyladenine和Necrostatin-1抑制LPS/zVAD和PolyI: C/zVAD誘導(dǎo)的骨髓巨噬細(xì)胞(BMDM)細(xì)胞死亡。

Chen YS, et al. Mol Cells. 2022 Apr 30;45(4):257-272.

Z-VAD-FMK

3-Methyladenine和Z-VAD-FMK聯(lián)合應(yīng)用證實(shí)了細(xì)胞程序性死亡在pristimerin介導(dǎo)的抗癌作用中的重要作用。

Al-Tamimi M, et al. Biomed Pharmacother. 2022 Dec;156:113950.

Fer-1 (Ferrostatin-1)

3-Methyladenine和Ferrostatin-1可消除丙烯酰胺(ACR)誘導(dǎo)的軟骨細(xì)胞細(xì)胞死亡。

Wang H, et al. Exp Ther Med. 2023 Apr 12;25(6):246.

3-MA (3-Methyladenine)相關(guān)產(chǎn)品

相關(guān)靶點(diǎn)	p110α p110β p110δ p110γ C2β Vps34	點(diǎn)擊展開(kāi)
相關(guān)產(chǎn)品	LY294002 Dactolisib (BEZ235) Pictilisib (GDC-0941) Wortmannin Buparlisib (BKM120) PI-103 TGX-221 ZSTK474 Omipalisib (GSK2126458) A66 PF-04691502 PIK-75 HCl IC-87114 740 Y-P (PDGFR 740Y-P) AS-605240 Taselisib (GDC 0032) SAR405 Apitolisib (GDC-0980) VPS34-IN1 PIK-90 AZD6482 Gedatolisib (PKI-587) HS-173 AS-252424 Eganelisib (IPI-549) VS-5584 (SB2343) GSK2636771 AZD8186 GSK1059615 Pilaralisib (XL147) TG100-115 CZC24832 Voxtalisib (XL765) XL147 analogue AS-604850 BGT226 (NVP-BGT226) maleate Quercetin Dihydrate VPS34 inhibitor 1 (Compound 19) Samotolisib (LY3023414) Voxtalisib (XL765) Analogue AMG319 Nemiralisib Bimiralisib (PQR309) CH5132799 PKI-402 TG100713 Paxalisib (GDC-0084) SF2523 Serabelisib (TAK-117) GDC-0326 GNE-317 Inavolisib (GDC-0077) PIK-294 PI-3065 AZD8835 Seletalisib (UCB-5857) leniolisib (CDZ 173) (E)-Akt inhibitor-IV Gallein CAY10505 Selective PI3Kδ Inhibitor 1 (compound 7n) PF-4989216 GNE-477 GNE-493	點(diǎn)擊展開(kāi)
相關(guān)化合物庫(kù)	激酶抑制劑庫(kù) PI3K/Akt 抑制劑庫(kù) 凋亡分子化合物庫(kù) 細(xì)胞周期化合物庫(kù) NF-κB信號(hào)通路庫(kù)	點(diǎn)擊展開(kāi)

細(xì)胞實(shí)驗(yàn)數(shù)據(jù)示例

細(xì)胞系	實(shí)驗(yàn)類(lèi)型	給藥濃度	孵育時(shí)間	活性描述	文獻(xiàn)信息
SMMC-7721	Apoptosis Assay	5mM	24h	attenuates TNF-α protection against serum starvation-mediated apoptosis	24066693
HO8910	Apoptosis Assay	10mM	12h	enhances B19-induced apoptosi	23983610
MCF7	Function Assay	5mM	24h	increases CuO induced cell death	23962629
HONE-1?	Function Assay	5mM	1h	represses 6r-mediated ROS production	23892358
HeLa	Function Assay	10mM	2h	suppresses LC3 II expressison	23864738
HepG2	Function Assay	10mM	24h	inhibits siTIGAR- and HBSS-induced autophagy	23817040
SH-SY5Y	Function Assay	1mM	24h	inhibits the autophagy induced by TOCP	23743148
SH-SY5Y	Apoptosis Assay	5mM	1h	abolishes celastrol neuroprotective effect	23619395
PC12?	Function Assay	10mM	24h	?inhibits chymotrypsin-like proteasomal activity.	23603979
OV2008	Apoptosis Assay	5mM	24h	converts FTY720 with CDDP into an additive effect towards killing ovarian cancer cells	23592281
A2780	Apoptosis Assay	5mM	24h	converts FTY720 with CDDP into an additive effect towards killing ovarian cancer cells	23592281
1321N1	Cytotoxicity Assay	5mM	24h	protects cell against PCN-induced toxicity	23525265
Saos-2?	Apoptosis Assay	1mM	96h	increases cell death induced by PCX	23563171
SH-SY5Y	Cytotoxicity Assay	5mM	24h	increases PCN toxicity	23525265
HT-29?	Function Assay	1mM	48/96h	inhibits AMPK induces autophagic cell death	23508272
OR6	Function Assay	10mM	72h	suppresses HCV replication and formation of autophagosomes	23395875
Hela?	Function Assay	5mM	24h	inhibits starvation-induced autophagy	23395679
MCF-7?	Function Assay	5mM	24h	inhibits starvation-induced autophagy	23395679
HUVECs	Function Assay	3mM	24h	blocks the protective effect of resveratrol by inhibiting autophagy	23358928
T24	Function Assay	10mM	1h	reduces the cleavage of LC3 after baicalin treatment	23354080
U251MG?	Function Assay	3mM	1h	suppresses LC3-II protein expression	23338618
GTL-16?	Apoptosis Assay	5mM	24h	reduces cell viability as compared to cells treated with MET inhibitors	23313490
T-47D	Function Assay	10mM	2h	inhibits autophagy process and increases rapamycin induced apoptosis	23300026
PaCa44	Apoptosis Assay	2.5mM	1h	reduces?genipin-mediated apoptosis	23124112
MDA-MB231	Function Assay	5mM	1h	increases resveratrol-mediated caspase activation and cell death	23088850
SK-HEP-1	Apoptosis Assay	10mM	1h	protects against autophagy and induces apoptosis in bufalin-treated cells	22858649
HeLa	Apoptosis Assay	10mM	2h	decreases cell viability co-treatment with PEI	23000135
HepG2	Apoptosis Assay	3mM	5h	reduces cell apoptosis induced by QDs	22836595
MCF-7?	Function Assay	2mM	24h	inhibits autophagy induced by TM?	24970676
MCF-7?	Function Assay	2mM	48h	promotes TM-induced cell death	24970676
MDA-MB-231	Function Assay	2mM	24h	inhibits autophagy induced by TM?	24970676
MDA-MB-231	Function Assay	2mM	48h	promotes TM-induced cell death	24970676
PANC-1?	Apoptosis Assay	1mM	48h	enhances bortezomib-induced cell viability loss	24842158
MDA-MB 231	Apoptosis Assay	5mM	0.5h	modulates Tocomin??induced apoptosis	24830781
Microglia?	Apoptosis Assay	5mM	24h	decreases hypoxia-induced cell death	24818601
HepG2	Function Assay	5mM	4h	increases cellular levels of HL?	24713587
A2780cp	Apoptosis Assay	2.5mM	1h	enhances cisplatin-induced cell death	24817946
U2OS	Growth Inhibition Assay	10mM	24h	intensifies the growth inhibition induced by Dox	24639013
HCT116	Apoptosis Assay	5mM	24h	enhances apigenin-induced cell death	24626522
PC-3?	Apoptosis Assay	2mM	2h	increases ORI-induced cell death	22745580
MCF-7?	Apoptosis Assay	0.1mM	6h	enhances sirtinol-induced apoptosis	22751989
U251?	Apoptosis Assay	5mM	24h	increases S1-induced cell death	22579788
HeLa?	Apoptosis Assay	5mM	24h	induces caspase-dependent cell death	22545128
pDCs	Function Assay	10mM	0.5h	?reduces the induction of IFN-α by ssRNA40	22396599
A549	Function Assay	0.1mM	24h	suppresses SU11274-induced cell death	22466960
BGC-823	Function Assay	5mM	2h	inhibits the rate of autophagic cells	22322152
U937	Function Assay	2mM	12h	decreases the autophagy ratio?	22155150
Marc-145	Function Assay	5mM	12/24/36h	reduces the PRRSV titers and the protein expression	22119900
HBx?	Apoptosis Assay	10mM	48h	increases cell death	22020078
MCF-7	Function Assay	10mM	48h	blocks autophagy induced by bortezomib	21931937
RMPI8226?	Function Assay	5mM	1h	suppresses the level of autophagy under nutrient depletion	21915620
PC12/TetOn	Function Assay	0.1/1mM	18h	leads to α-syn(WT) accumulation, toxicity, and oligomer formation?	21906659
HeLa?	Cytotoxicity Assay	2mM	24h	inhibites the cytotoxicity of silibinin to HeLa cells.	21875385
Jurkat	Function Assay	10mM	1h	decreases the expression of LC3-II and the formation of autophagosomes	21864037
K562	Function Assay	10mM	1h	decreases the expression of LC3-II and the formation of autophagosomes	21864037
Hep3B	Apoptosis Assay	5mM	24h	attenuates TNF-α protection against serum starvation-mediated apoptosis	24066693
H460	Function Assay	10mM	4h	increases cisplatin-induced cell death	24173208
A549	Function Assay	10mM	4h	inhibits autophagy induced by irradiation	24142735
H1299?	Function Assay	10mM	4h	increases cisplatin-induced cell death	24173208
WiDr	Function Assay	10mM	1h	inhibits PCBL-induced LC3 II expression	24190489
LoVo?	Apoptosis Assay	5mM	48h	enhances DCA-induced apoptosis.	24201812
HepG2 E47	Function Assay	2.5mM	48h	increases the toxicity of AA, BSO, and CCl4	24273738
RKO	Function Assay	2mM	1h	enhances cell death by geldanamycin	24291777
Hep3B	Apoptosis Assay	2mM	12h	inhibits AZD8055-induced cell death	24297300
ACHN-5968	Apoptosis Assay	5mM	3h	enhances paclitaxel-mediated apoptosis?	24305604
Huh7	Apoptosis Assay	2mM	12h	inhibits AZD8055-induced cell death	24297300
UOK257	Apoptosis Assay	5mM	3h	enhances paclitaxel-mediated apoptosis?	24305604
ECSCs?	Apoptosis Assay	10mM	4h	decreases rapamycin-treated apoptosis	24319109
MCF-7?	Function Assay	10mM	24h	inhibits the autophagy induced by chemotherapy drugs	24315578
SGC-7901?	Apoptosis Assay	2mM	1h	increases CA-4 induced apoptosis	24321340
SMMC-7721	Apoptosis Assay	2mM	1h	increases CA-4 induced apoptosis	24321340
T24	Apoptosis Assay	5mM	1.5h	potentiates celecoxib-induced apoptosis	24349176
NTUB1	Apoptosis Assay	5mM	1.5h	potentiates celecoxib-induced apoptosis	24349176
MG-63	Apoptosis Assay	10mM	12h	enhances DP-induced apoptosis	24358301
MG-63	Apoptosis Assay	0.5/1mM	32h	enhances salinomycin-induced cell apoptosis	24358342
MG-63	Function Assay	0.5/1mM	48h	induces salinomycin-induced cell viability loss	24358342
U2OS?	Function Assay	0.5/1mM	48h	induces salinomycin-induced cell viability loss	24358342
HGC-27	Function Assay	10mM	1h	inhibits the cell viability loss by RAD001 or MK-2206	24416349
HCT116?	Apoptosis Assay	5mM	24h	enhances the apoptosis induced by apigenin	24626522
A549	Apoptosis Assay	10mM	48h	accelerates the reduction of cell viability induced by PTX	24626722
Saos-2	Apoptosis Assay	10mM	24h	intensifies the growth inhibition of the U2OS cells induced by Dox	24639013
U2OS	Apoptosis Assay	10mM	24h	intensifies the growth inhibition of the U2OS cells induced by Dox	24639013
HepG2	Function Assay	5mM	4h	increases HL release	24713587
A549	Apoptosis Assay	5mM	48h	decreases the percentage of cell death and expression levels of caspase-3, Beclin-1 and LC3-II	24706303
A2780cp	Apoptosis Assay	2.5mM	1h	enhances cisplatin-induced cell death	24817946
Microglia?	Apoptosis Assay	5mM	24h	decreases hypoxia-induced cell death	24818601
HT-29?	Apoptosis Assay	1mM	48h	enhances bortezomib-induced cell viability loss	24842158
MDR	Apoptosis Assay	10mM	6h	strengthens the power of anticancer drugs	25019701
H157	Function Assay	5mM	2h	suppresses SPC induced accumulation of LC3-II	25285628
A549?	Function Assay	5mM	2h	suppresses SPC induced accumulation of LC3-II	25285628
A2780cp?	Growth Inhibition Assay	1mM	1h	increases cisplatin-induced cell death	25322694
NBL-W-S?	Apoptosis Assay	1mM	6h	increases cell apoptosis induced by GANT-61	25323222
NBL-W-S?	Growth Inhibition Assay	1mM	6h	enhances GANT-61 toxicity	25323222
A549?	Apoptosis Assay	5mM	2h	inhibits BDMC-induced apoptotic cell death	25716561
95D	Apoptosis Assay	5mM	2h	inhibits BDMC-induced apoptotic cell death	25716561
A549?	Growth Inhibition Assay	3mM	2h	reduces growth inhibitory effect of BDMC	25716561
95D	Growth Inhibition Assay	3mM	2h	reduces growth inhibitory effect of BDMC	25716561
Nara-H	Growth Inhibition Assay	5mM	48h	enhances temsirolimusmediated suppression of Nara-H cell proliferation	21805033
HUVECs	Function Assay	10mM	0.5h	decreases the AGE-BSAinduced autophagy leve	21468592
HepG2	Apoptosis Assay	2mM	1h	enhances radiation-induced cell death	21453691
U-2 OS	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for U-2 OS cells	29435139
A673	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for A673 cells	29435139
DAOY	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for DAOY cells	29435139
Saos-2	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for Saos-2 cells	29435139
BT-37	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for BT-37 cells	29435139
RD	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for RD cells	29435139
SK-N-SH	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SK-N-SH cells	29435139
BT-12	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for BT-12 cells	29435139
MG 63 (6-TG R)	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for MG 63 (6-TG R) cells	29435139
NB1643	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for NB1643 cells	29435139
OHS-50	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for OHS-50 cells	29435139
Rh41	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for Rh41 cells	29435139
Rh30	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for Rh30 cells	29435139
SJ-GBM2	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SJ-GBM2 cells	29435139
SK-N-MC	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SK-N-MC cells	29435139
NB-EBc1	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for NB-EBc1 cells	29435139
LAN-5	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for LAN-5 cells	29435139
Rh18	qHTS assay			qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for Rh18 cells	29435139
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生物活性

產(chǎn)品描述

靶點(diǎn)

Autophagy ^[1]	Vps34 ^[1] (HeLa cells)	PI3Kγ ^[1] (HeLa cells)
	25 μM	60 μM

體外研究(In Vitro)
體外研究活性	3-MA對(duì)Vps34輕微的偏向性可能是由于Vps34中某個(gè)特異性的疏水環(huán)形結(jié)構(gòu)可以環(huán)繞在3-MA的3-甲基基團(tuán)外面造成的^[1] 。據(jù)報(bào)道對(duì)于正常培養(yǎng)和饑餓處理的癌細(xì)胞3-MA都能引起細(xì)胞死亡。3-MA還可以不通過(guò)抑制自吞噬來(lái)抑制細(xì)胞遷移和入侵，這表明3-MA 除了抑制自吞噬之外還有其它生物功能。3-MA可以造成半胱天冬酶依賴(lài)的細(xì)胞死亡，這一功能與它對(duì)自吞噬的抑制無(wú)關(guān)。用5mM 3-MA處理葡萄糖饑餓Hela細(xì)胞可降低gfp-lc3陽(yáng)性細(xì)胞比例至23%。3-MA處理細(xì)胞12到48小時(shí)內(nèi) LC3-I 的水平上升而LC3-II 水平下降。LC3-I轉(zhuǎn)為為 LC3-II 的過(guò)程被3-MA抑制了。 2.5 mM 或5 mM 3-MA 處理HeLa 細(xì)胞一天并不影響細(xì)胞存活率, 而 10 mM 3-MA 處理一天會(huì)造成細(xì)胞存活率下降25.0% 。 2.5, 5 或 10 mM 3-MA 處理細(xì)胞兩天分別使細(xì)胞存活率下降 11.5%, 38.0% 和79.4% 。3-MA 降低細(xì)胞存活率的作用具有時(shí)間和劑量依賴(lài)特性。3-MA 明顯縮短nocodazole誘導(dǎo)的前中期阻斷時(shí)間^[2]。3-MA 通過(guò)抑制自吞噬來(lái)抑制SU11274誘導(dǎo)的細(xì)胞死亡^[3]。在野生型MEF細(xì)胞中延長(zhǎng) 3-MA處理時(shí)間 (最多 9 小時(shí)) 明顯造成 LC3 I 到 II 轉(zhuǎn)換。延長(zhǎng) 3-MA處理時(shí)間會(huì)明顯增加GFP-LC3的聚集而wortmannin不具有此功能。3-MA介導(dǎo)的LC3 轉(zhuǎn)換和游離 GFP 釋放是ATG7依賴(lài)的。 3-MA處理會(huì)導(dǎo)致 p62 蛋白水平明顯升高。即使在Atg5−/− 的MEF細(xì)胞中3-MA 也會(huì)使p62 水平升高，就像在DOX介導(dǎo)的ATG5缺失的細(xì)胞中一樣。3-MA以不同方式抑制 I 型和 III型 PI3K。與野生型細(xì)胞相比，在Tsc2−/−細(xì)胞中3-MA誘導(dǎo)的 LC3 I 到 LC3 II 轉(zhuǎn)換過(guò)程大幅下降。3-MA會(huì)破壞mTOR復(fù)合物1的拮抗自吞噬功能^[4]。
細(xì)胞實(shí)驗(yàn)	細(xì)胞系	HeLa 細(xì)胞系
	濃度	1-10 mM
	孵育時(shí)間	3 分鐘
	方法	用臺(tái)盼藍(lán)染色法分析細(xì)胞存活率（如HeLa細(xì)胞）。簡(jiǎn)言之, 經(jīng)過(guò)3-MA處理后將貼壁的與漂浮的細(xì)胞都用PBS( pH 7.4)重懸，密度控制在每毫升1-2×10⁶。加入等體積的0.4% 臺(tái)盼藍(lán)并徹底混勻。室溫孵育3分鐘后用血細(xì)胞計(jì)數(shù)器對(duì)細(xì)胞計(jì)數(shù)分析。
實(shí)驗(yàn)圖片	檢測(cè)方法	檢測(cè)指標(biāo)	實(shí)驗(yàn)圖片	PMID
	Western blot	α-SMA / TGF-β / LC-3BI / LC-3B II / Beclin-1 / NF-κB p65 caspase-3 / caspase-9 / PARP VEGF APP / BACE1 / ADAM17 / Presenilin 1 / Presenilin 2 / Nicastrin / APH-1 / Pen-2 / LC3-1 / LC3-2		29296191
	Immunofluorescence	LC3 / Hif-α / COX2		29039446
	Growth inhibition assay	Cell viability		26934124

體內(nèi)研究(In Vivo)
體內(nèi)研究活性	3-Methyladenine (3-MA)可以通過(guò)對(duì)磷酸肌醇3磷酸激酶(PI3K) 的作用來(lái)阻斷自吞噬, 而PI3K的活性對(duì)于自噬體形成早期膜池的成核和組裝是必須的。與SAH 處理組相比3-MA并不會(huì)改變出血的程度。與SAH +對(duì)照成分組相比經(jīng)過(guò)3-MA預(yù)處理后會(huì)明顯加重神經(jīng)病學(xué)癥狀。3-MA處理會(huì)減少自吞噬發(fā)生^[5]。相反地,在SAH + 3-MA組里斷裂的半胱天冬酶表達(dá)量明顯上調(diào)，與此一致的是與SAH + 對(duì)照成分組相比，SAH + 3-MA組中右腦皮層里原位末端標(biāo)記陽(yáng)性細(xì)胞數(shù)量明顯增多^[5]。
動(dòng)物實(shí)驗(yàn)	Animal Models	體重 300-350 g 的成年雄性Sprague–Dawley 大鼠
	Dosages	400 nM
	Administration	腦室給藥

參考文獻(xiàn)
[1] Miller S, et al. Autophagy. 2010, 6(6), 805-807. [2] Hou H, et al. PLoS One. 2012, 7(4), e35665. [3] Liu Y, et al. J Pharmacol Sci. 2012, 118(4), 423-432. [4] Wu YT, et al. J Biol Chem. 2010, 285(14), 10850-10861. [5] Jing CH, et al. Neuroscience. 2012. + 展開(kāi)

參考文獻(xiàn)

[1] Miller S, et al. Autophagy. 2010, 6(6), 805-807.
[2] Hou H, et al. PLoS One. 2012, 7(4), e35665.
[3] Liu Y, et al. J Pharmacol Sci. 2012, 118(4), 423-432.

[4] Wu YT, et al. J Biol Chem. 2010, 285(14), 10850-10861.
[5] Jing CH, et al. Neuroscience. 2012.

+ 展開(kāi)

化學(xué)信息&溶解度

分子量	149.15	分子式	C₆H₇N₅
CAS號(hào)	5142-23-4	SDF	Download 3-MA (3-Methyladenine) SDF
Smiles	CN1C=NC(=N)C2=C1N=CN2
儲(chǔ)存條件(自收到貨起)	3年 -20°C 粉狀	此產(chǎn)品性質(zhì)不穩(wěn)定，需現(xiàn)配現(xiàn)用!建議您購(gòu)買(mǎi)分裝規(guī)格，或者在收到貨后進(jìn)行分裝。

體外溶解度
批次：

DMSO : 10 mg/mL ( (67.04 mM) Warmed with 50°C water bath; Ultrasonicated; ；DMSO吸濕會(huì)降低化合物溶解度，請(qǐng)使用新開(kāi)封DMSO)

Ethanol : 10 mg/mL (67.04 mM)

Water : 4 mg/mL (26.81 mM)

DMSO : 16 mg/mL ( (107.27 mM) Warmed with 50°C water bath; Ultrasonicated; ；DMSO吸濕會(huì)降低化合物溶解度，請(qǐng)使用新開(kāi)封DMSO)

Water : 10 mg/mL (67.04 mM) Warmed with 50°C water bath; Ultrasonicated;

Ethanol : 8 mg/mL (53.63 mM)

摩爾濃度計(jì)算器

體內(nèi)溶解度
批次：

現(xiàn)配現(xiàn)用，請(qǐng)按從左到右的順序依次添加，澄清后再加入下一溶劑

動(dòng)物體內(nèi)配方計(jì)算器

實(shí)驗(yàn)計(jì)算

摩爾濃度計(jì)算器

質(zhì)量	濃度	體積	分子量
=	×	×

稀釋計(jì)算器分子量計(jì)算器

動(dòng)物體內(nèi)配方計(jì)算器（澄清溶液）

第一步：請(qǐng)輸入基本實(shí)驗(yàn)信息（考慮到實(shí)驗(yàn)過(guò)程中的損耗，建議多配一只動(dòng)物的藥量）

給藥劑量 mg/kg 動(dòng)物平均體重 g 每只動(dòng)物給藥體積 μL 動(dòng)物數(shù)量只

第二步：請(qǐng)輸入動(dòng)物體內(nèi)配方組成（配方適用于不溶于水的藥物；不同批次藥物配方比例不同，請(qǐng)聯(lián)系Selleck為您提供正確的澄清溶液配方）

% DMSO + % + % Tween 80 + % ddH2O

%DMSO+ %

計(jì)算結(jié)果：

工作液濃度： mg/ml；

DMSO母液配制方法： mg 藥物溶于μL DMSO溶液（母液濃度mg/mL，注：如該濃度超過(guò)該批次藥物DMSO溶解度，請(qǐng)先聯(lián)系Selleck）；

體內(nèi)配方配制方法：取μL DMSO母液，加入μL PEG300，混勻澄清后加入μL Tween 80，混勻澄清后加入μL ddH₂O，混勻澄清。

體內(nèi)配方配制方法：取μL DMSO母液，加入μL Corn oil，混勻澄清。

注意：1. 首先保證母液是澄清的；
2.一定要按照順序依次將溶劑加入，進(jìn)行下一步操作之前必須保證上一步操作得到的是澄清的溶液，可采用渦旋、超聲或水浴加熱等物理方法助溶。

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常見(jiàn)問(wèn)題及建議解決方法

問(wèn)題 1:
I'm also wondering whether it can be dissolved in water,or maybe something like culture medium,normal saline solution to form 10mM solution

回答：
As the reference (http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal. pone.0035665), 3-MA, which was found to inhibit autophagy at concentrations ranging from 1 to 10 mM was directly dissolved into the culture medium at the indicated concentrations. And we tested the solubility of S2767, and found the solubility of 3-MA in DMEM is 31 mg/mL at about 40°C.

C1=C0/X	C1:	LOG(C1):
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C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

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