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FCCP
別名: Trifluoromethoxy carbonylcyanide phenylhydrazone, Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone 中文名稱：碳酰氰-4-三氟甲氧基苯腙
FCCP (Trifluoromethoxy carbonylcyanide phenylhydrazone, Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone)在線粒體中是一種有效的氧化磷酸化的解偶聯(lián)劑，通過轉(zhuǎn)運質(zhì)子破壞ATP的合成。
FCCP Chemical Structure
CAS: 370-86-5
產(chǎn)品質(zhì)控
批次：
純度：
99.99%
99.99
FCCP相關(guān)產(chǎn)品
| 相關(guān)產(chǎn)品 | IACS-010759 (IACS-10759) Gboxin VLX600 S-Gboxin | 點擊展開 |
|---|---|---|
| 相關(guān)化合物庫 | 自噬化合物庫 凋亡分子化合物庫 鐵死亡化合物庫 細(xì)胞焦亡化合物庫 線粒體靶向化合物庫 | 點擊展開 |
細(xì)胞實驗數(shù)據(jù)示例
| 細(xì)胞系 | 實驗類型 | 給藥濃度 | 孵育時間 | 活性描述 | 文獻(xiàn)信息 |
|---|---|---|---|---|---|
| T47D | Function assay | 0.3 uM | 15 mins | Decrease in mitochondrial membrane potential in human T47D cells 0.3 uM after 15 mins by TMRM assay | 20929261 |
| T47D | Function assay | 3 uM | 15 to 20 mins | Decrease in mitochondrial membrane potential in human T47D cells at 3 uM after 15 to 20 mins by TMRM assay | 22938093 |
| SH-SY5Y | Function assay | 10 uM | 5 mins | Inhibition of SOC in human SH-SY5Y cells assessed as reduction in thapsigargin-induced Ca2+ influx at 10 uM pre-incubated for 5 mins with 0.2 uM CsA followed by compound addition by FURA-2AM dye based fluorescence assay | 25265024 |
| SH-SY5Y | Function assay | 10 uM | 10 mins | Induction of mitochondrial membrane potential loss in human SH-SY5Y cells at 10 uM incubated for 10 mins in presence of 0.2 uM CsA by TMRE dye based assay | 25265024 |
| T47D | Function assay | 0.3 uM | 3 to 12 mins | Increase in oxygen consumption rate of mitochondrial state 4 respiration in human T47D cells assessed as reinitiation of oligomycin-stalled cellular respiration at 0.3 uM incubated for 3 to 12 mins by Clark-type oxygen electrode assay | 26637046 |
| T47D | Function assay | 0.3 uM | 30 mins | Effect on mitochondrial membrane potential in human T47D cells at 0.3 uM after 30 mins by TMRM dye based fluorescence microscopy | 26637046 |
| HCT116 | Function assay | 2 uM | 30 mins | Induction of AMPK phosphorylation at Thr-172 residue in human HCT116 cells at 2 uM after 30 mins in glucose supplemented media by immunoblot method | 28233680 |
| HCT116 | Function assay | 2 uM | 30 mins | Induction of AMPK phosphorylation at Thr-172 residue in human HCT116 cells at 2 uM after 30 mins in absence of glucose by immunoblot method | 28233680 |
| KOPN8 | Function assay | 10 uM | 0.3 hrs | Induction of mitochondrial membrane potential loss in human KOPN8 cells at 10 uM after 0.3 hrs by TMRM staining based flow cytometric analysis | 31084028 |
| T47D | Function assay | 1 to 10 uM | Inhibition of HIF1-mediated induction of secreted VEGF level in 1, 10-phenanthroline-stimulated human T47D cells at 1 to 10 uM by ELISA | 20929261 | |
| MDA-MB-231 | Cytotoxicity assay | 1 uM | Cytotoxicity against human MDA-MB-231 cells assessed as inhibition of cell proliferation/viability at 1 uM | 23245650 | |
| MDA-MB-231 | Cytotoxicity assay | 0.1 to 3 uM | Cytotoxicity against human MDA-MB-231 cells assessed as inhibition of cell proliferation/viability at 0.1 to 3 uM in presence of 0.1 uM rotenone mitochondrial electron transport inhibitor | 23245650 | |
| MDA-MB-231 | Function assay | 0.3 uM | Stimulation of oligomycin-induced state 4 respiration in human MDA-MB-231 cells at 0.3 uM | 23245650 | |
| T47D | Function assay | 0.1 to 3 uM | Effect on cellular respiration in human T47D cells assessed as increase in oxygen consumption at 0.1 to 3 uM | 22938093 | |
| T47D | Function assay | 10 to 30 uM | Stimulation of state 4 cellular respiration in human T47D cells at 10 to 30 uM in presence of oligomycin | 22938093 | |
| T47D | Function assay | 0.3 to 1 uM | Stimulation of state 4 cellular respiration in human T47D cells at 0.3 to 1 uM in presence of oligomycin | 22938093 | |
| Hep3B | Function assay | 10 uM | Stimulation of state 4 cellular respiration in human Hep3B cells at 10 uM in presence of oligomycin | 22938093 | |
| Hep3B | Function assay | 1 uM | Stimulation of state 4 cellular respiration in human Hep3B cells at 1 uM in presence of oligomycin | 22938093 | |
| TA3/Ha | Function assay | 6 uM | Induction of NAD(P)H oxidation in mouse TA3/Ha cells assessed as reduction of NAD(P)H/NAD(P)+ ratio at 6 uM by spectrofluorometer analysis | 24568614 | |
| T47D | Function assay | 0.3 uM | Increase in oxygen consumption rate in digitonin permeabilized human T47D cells assessed as reinitiation of sodium azide-stalled cellular respiration at 0.3 uM by oxytherm Clark-type electrode assay in presence of ascorbate | 26637046 | |
| DLD1 | Function assay | 1 uM | Induction of mitochondrial dysfunction in human DLD1 cells assessed as reduction in mitochondrial ATP production at 1 uM by Seahorse XF real-time assay | 31774672 | |
| DLD1 | Function assay | 1 uM | Induction of mitochondrial dysfunction in human DLD1 cells assessed as increase in glycolytic ATP production at 1 uM by Seahorse XF real-time assay | 31774672 | |
| LS174T | Function assay | 1 uM | Induction of mitochondrial dysfunction in human LS174T cells assessed as reduction in mitochondrial ATP production at 1 uM by Seahorse XF real-time assay | 31774672 | |
| LS174T | Function assay | 1 uM | Induction of mitochondrial dysfunction in human LS174T cells assessed as increase in glycolytic ATP production at 1 uM by Seahorse XF real-time assay | 31774672 | |
| DLD1 | Function assay | 1 uM | Uncoupling of mitochondrial oxidative phosphorylation in human DLD1 cells at 1 uM in presence of oligomycin A by seahorse XFe96 analyser based assay | 31774672 | |
| DLD1 | Function assay | 1 uM | Uncoupling of mitochondrial oxidative phosphorylation in human DLD1 cells assessed as increase in oxygen consumption rate at 1 uM in presence of oligomycin A by seahorse XFe96 analyser based assay | 31774672 | |
| HEK293 | Function assay | 1 to 3 uM | Inhibition of LiCl-activated Wnt signaling in HEK293 cells at 1 to 3 uM by TOPFlash reporter gene assay | 31774672 | |
| T47D | Function assay | Inhibition of hypoxia-induced HIF1 activation in human T47D cells by HRE3-TK-luciferase reporter gene assay, IC50=0.51μM | 20929261 | ||
| T47D | Function assay | Inhibition of 1, 10-phenanthroline-induced HIF1 activation in human T47D cells by HRE3-TK-luciferase reporter gene assay, IC50=0.31μM | 20929261 | ||
| HepG2 | Function assay | Luciferase/luciferin-expressing antifolate-resistant parasites were used to infect a culture of HepG2 cells that were pre-incubated with compounds. Infected hepatocytes emit light due to the luciferase reaction. Assay results are presented as the percent , IC50=0.245μM | ChEMBL | ||
| 點擊查看更多細(xì)胞系數(shù)據(jù) | |||||
生物活性
| 產(chǎn)品描述 | FCCP (Trifluoromethoxy carbonylcyanide phenylhydrazone, Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone)在線粒體中是一種有效的氧化磷酸化的解偶聯(lián)劑，通過轉(zhuǎn)運質(zhì)子破壞ATP的合成。 | ||
|---|---|---|---|
| 靶點 |
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| 體外研究(In Vitro) | ||||
| 體外研究活性 | 在體外實驗中，F(xiàn)CCP的處理能誘導(dǎo)細(xì)胞內(nèi)Ca2+迅速升至2倍，同時抑制蛋白合成速率。對蛋白翻譯速率的抑制作用與eIF2α的磷酸化增加以及依賴雙鏈RNA的蛋白激酶活性增加有關(guān)[1]。 FCCP還可輕微地減少ATP以及活性氧水平，提高線粒體基因如Tfam和COXIV的表達(dá)，誘導(dǎo)老鼠造血干細(xì)胞的靜態(tài)形態(tài)特征以及抑制TGF-β的信號轉(zhuǎn)導(dǎo)[2]。 |
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|---|---|---|---|---|
| 細(xì)胞實驗 | 細(xì)胞系 | PC12細(xì)胞 | ||
| 濃度 | 30 μM | |||
| 孵育時間 | 30 min, 1h, 2h | |||
| 方法 | 用直徑為24-mm的多孔板，加入含0.175 Ci/mmol的[3H]methionine的新鮮培養(yǎng)液，37℃孵育30分鐘。在不同的時間段對PC12細(xì)胞處理以FCCP，測定蛋白質(zhì)合成速率。 |
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| 體內(nèi)研究(In Vivo) | ||
| 體內(nèi)研究活性 | FCCP的處理在8細(xì)胞期的小鼠胚胎中可顯著降低線粒體膜電位、ATP的生成以及囊胚中細(xì)胞內(nèi)細(xì)胞團的數(shù)量，對胚泡發(fā)育沒有影響。在胚胎線粒體功能受到干擾的同時，在胚胎植入后，雌性后代的出生體重下降，抑制持續(xù)到斷奶階段。盡管FCCP處理過的雄性小鼠也和雌性小鼠一樣，葡萄糖耐受量降低。但是它們的胰島素敏感度和肥胖度在4-14周內(nèi)無變化。在預(yù)壓胚中，線粒體功能降低，然后減少ATP的輸出，將影響其后代表型[3]。 |
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|---|---|---|
化學(xué)信息&溶解度
| 分子量 | 254.17 | 分子式 | C10H5F3N4O |
| CAS號 | 370-86-5 | SDF | Download FCCP SDF |
| Smiles | C1=CC(=CC=C1NN=C(C#N)C#N)OC(F)(F)F | ||
| 儲存條件(自收到貨起) | |||
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體外溶解度 |
DMSO : 6 mg/mL ( (23.6 mM) ；DMSO吸濕會降低化合物溶解度，請使用新開封DMSO) Water : Insoluble Ethanol : Insoluble |
摩爾濃度計算器 |
|
體內(nèi)溶解度 現(xiàn)配現(xiàn)用，請按從左到右的順序依次添加，澄清后再加入下一溶劑 |
動物體內(nèi)配方計算器 | ||||
實驗計算
動物體內(nèi)配方計算器（澄清溶液）
第一步：請輸入基本實驗信息（考慮到實驗過程中的損耗，建議多配一只動物的藥量）
mg/kg
g
μL
只
第二步：請輸入動物體內(nèi)配方組成（配方適用于不溶于水的藥物；不同批次藥物配方比例不同，請聯(lián)系Selleck為您提供正確的澄清溶液配方）
% DMSO
%
% Tween 80
% ddH2O
%DMSO
%
計算結(jié)果：
工作液濃度： mg/ml；
DMSO母液配制方法： mg 藥物溶于μL DMSO溶液（母液濃度mg/mL，注：如該濃度超過該批次藥物DMSO溶解度，請先聯(lián)系Selleck）；
體內(nèi)配方配制方法：取μL DMSO母液，加入μL PEG300，混勻澄清后加入μL Tween 80，混勻澄清后加入μL ddH2O，混勻澄清。
體內(nèi)配方配制方法：取μL DMSO母液，加入μL Corn oil，混勻澄清。
注意：1. 首先保證母液是澄清的；
2.一定要按照順序依次將溶劑加入，進(jìn)行下一步操作之前必須保證上一步操作得到的是澄清的溶液，可采用渦旋、超聲或水浴加熱等物理方法助溶。
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