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Nocodazole

別名: R17934, Oncodazole, NSC238159 中文名稱：諾考達唑

Nocodazole是一種微管聚合的快速可逆抑制劑，也抑制Abl、Abl(E255K)和Abl(T315I)，對應的IC50值分別為0.21 μM，0.53 μM和0.64 μM。Nocodazole可提高CRISPR介導的同源重組效率，對增強精確的基因編輯效力具有加成作用。Nocodazole可誘導凋亡。

Nocodazole Chemical Structure

Nocodazole Chemical Structure

CAS: 31430-18-9

規(guī)格 價格 庫存 購買數(shù)量
10mM (1mL in DMSO) 794.43 現(xiàn)貨
10mg 580.33 現(xiàn)貨
50mg 2217.08 現(xiàn)貨
更大包裝 有超大折扣

400-668-6834

info@selleck.cn
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Nocodazole相關產(chǎn)品

細胞實驗數(shù)據(jù)示例

細胞系 實驗類型 給藥濃度 孵育時間 活性描述 文獻信息
HepG2 Function assay 0.1 to 10 uM 1 hr Inhibition of TNFalpha-induced NFkappaB (unknown origin)-dependent transcriptional activity expressed in human HepG2 cells at 0.1 to 10 uM incubated for 1 hr prior to TNFalpha induction measured after 6 hrs by dual-luciferase reporter gene assay 25827522
L1210 Function assay 0.3 uM 24 hrs Fraction of cell population of cultured lymphoid leukemia L1210 cells in mitosis at a dose of 0.3 uM after 24 hr, Mitotic index = 0.19 μM. 7131483
NIH3T3 Apoptosis assay 0.5 ug/ml 24 hrs Induction of apoptosis in mouse NIH3T3 cells assessed as accumulation at subG1 phase at 0.5 ug/ml after 24 hrs by flow cytometry 18247573
PC3M Apoptosis assay 0.5 ug/ml 24 hrs Induction of apoptosis in human PC3M cells assessed as accumulation at subG1 phase at 0.5 ug/ml after 24 hrs by flow cytometry 18247573
NRK Function assay 30 uM 60 mins Golgi-disturbing activity in golgi apparatus of rat NRK cells assessed as microtubule depolymerization-associated golgi fragmentation at 30 uM after 60 mins by Hoechst 3342 staining-based immunofluorescence microscopy 20189813
Vero Function assay 5 ug/ml 30 mins Effect on disruption of microtubules in african green monkey Vero cells at 5 ug/ml after 30 mins by immunofluorescence method 19182783
MCF7 Apoptosis assay 2.5 uM 48 hrs Induction of apoptosis in human MCF7 cells assessed as nuclear fragmentation at 2.5 uM after 48 hrs by DAPI staining 21194809
MCF7 Function assay 2.5 uM 48 hrs Inhibition of tubulin polymerization in human MCF7 cells assessed as disrupted microtubule organization at 2.5 uM after 48 hrs by microscopic analysis 21194809
A549 Apoptosis assay 10 to 100 nM 48 hrs Induction of apoptosis in human A549 cells assessed as early apoptotic cells at 10 to 100 nM after 48 hrs by annexin-V FITC/7-ADD staining-based flow cytometric analysis 21916509
A549 Apoptosis assay 10 to 100 nM 48 hrs Induction of apoptosis in human A549 cells assessed as necrotic cells at 10 to 100 nM after 48 hrs by annexin-V FITC/7-ADD staining-based flow cytometric analysis 21916509
A549 Cell cycle assay 1 uM 48 hrs Cell cycle arrest in human A549 cells assessed as accumulation at G2/M phase at 1 uM after 48 hrs by propidium iodide staining-based flow cytometric analysis relative to control 22361684
SKBR3 Function assay 50 to 100 nM 24 hrs Induction of Hsp27 phosphorylation in human SKBR3 cells at 50 to 100 nM after 24 hrs by SDS-PAGE analysis 22435708
SKBR3 Function assay 0.2 to 1 uM 24 hrs Induction of Hsp27 phosphorylation in human SKBR3 cells at 0.2 to 1 uM after 24 hrs by SDS-PAGE analysis 22435708
MCF7 Function assay 4 uM 24 hrs Inhibition of microtubule organization in human MCF7 cells at 4 uM after 24 hrs by immunofluorescence analysis 22982122
HeLa Function assay 1 uM 24 hrs Inhibition of tubulin polymerization in human HeLa cells assessed as tubulin soluble fraction at 1 uM after 24 hrs by immunohistochemistry analysis 23313639
HeLa Function assay 1 uM 24 hrs Mitotic arrest in human HeLa cells assessed as increase in accumulation at G2/M phase at 1 uM for 24 hrs by PI staining/FACS analysis 24530033
HeLa Cell cycle assay 2 uM 24 hrs Cell cycle arrest at G2/M phase in human HeLa cells assessed as increase in cyclin B1 expression at 2 uM after 24 hrs by immunoblot analysis relative to control 25499929
HeLa Cell cycle assay 2 uM 24 hrs Cell cycle arrest at G2/M phase in human HeLa cells assessed as soluble form of tubulin at 2 uM after 24 hrs by immunoblot analysis relative to control 25499929
DU145 Function assay 50 uM 48 hrs Disruption of microtubule network in human DU145 cells at 50 uM after 48 hrs by immunohistochemistry analysis 25615796
HeLa Function assay 25 uM 24 hrs Effect on PCM1 protein in human HeLa cells assessed as accumulation of pericentriolar material at 25 uM after 24 hrs by immunofluorescence microscopy 25050880
MCF7 Function assay 1 uM 4 hrs Induction of tubulin depolymerization in human MCF7 cells at 1 uM after 4 hrs by sedimentation assay and Western blotting method 25369367
DU145 Function assay 1.5 uM 48 hrs Reduction in mitochondrial membrane potential in human DU145 cells at 1.5 uM incubated for 48 hrs by JC1 dye staining based flow cytometry 26330077
SKBR3 Function assay 2.5 uM 24 hrs Induction of microtubule destabilization in human SKBR3 cells assessed as soluble fraction of protein at 2.5 uM after 24 hrs by Western blot analysis 26599530
HeLa Function assay 100 nM 4 hrs Inhibition of tubulin polymerization in human HeLa cells assessed as microtubule disruption in mitotic phase at 100 nM after 4 hrs by indirect immunofluorescence analysis 26641132
HeLa Function assay 100 nM 4 hrs Inhibition of tubulin polymerization in human HeLa cells assessed as microtubule disruption in interphase at 100 nM after 4 hrs by indirect immunofluorescence analysis 26641132
HeLa Function assay 0.1 uM 24 hrs Inhibition of tubulin polymerization in human HeLa cells assessed as shift of tubulin protein to soluble fraction at 0.1 uM after 24 hrs by Western blot analysis 29501940
PC3MLN4 Function assay 1 uM 24 hrs Upregulation of Cyclin-B1 protein expression in human PC3MLN4 cells at 1 uM after 24 hrs by Western blot method 29288939
PC3MLN4 Function assay 1 uM 48 hrs Disruption of microtubule dynamics in human PC3MLN4 cells at 1 uM after 48 hrs by immunofluorescence microscopic method 29288939
MCF7 Function assay 1 uM 4 hrs Induction of tubulin depolymerization in human MCF7 cells at 1 uM after 4 hrs by Western blot method 27689728
Jurkat Function assay 1 uM 4 hrs Induction of tubulin depolymerization in human Jurkat cells at 1 uM after 4 hrs by Western blot method 27689728
MDA-MB-231 Anticancer assay 48 hrs Anticancer activity against human MDA-MB-231 cells after 48 hrs by SRB assay, GI50 = 0.042 μM. 27130357
LT12 Antiproliferative assay 48 hrs Antiproliferative activity against rat LT12 cells after 48 hrs by XTT assay, IC50 = 0.04 μM. 21705223
LT12 Antiproliferative assay 48 hrs Antiproliferative activity against rat LT12 cells after 48 hrs by XTT assay, IC50 = 0.04 μM. 20537765
LT12 Antiproliferative assay 48 hrs Antiproliferative activity against LT12 cells by XTT assay after 48 hrs, IC50 = 0.04 μM. 17181164
P388 Antiproliferative assay 48 hrs Antiproliferative activity against mouse P388 cells after 48 hrs by XTT assay, IC50 = 0.04 μM. 19220018
NCI60 Growth inhibition assay 48 hrs Growth inhibition of human NCI60 cells incubated for 48 hrs by sulforhodamine B assay, GI50 = 0.0389 μM. 27956038
A549 Cytotoxicity assay 24 hrs Cytotoxicity against human A549 cells after 24 hrs by MTT assay, IC50 = 0.0366 μM. 20110137
PANC1 Anticancer assay 48 hrs Anticancer activity against human PANC1 cells after 48 hrs by SRB assay, GI50 = 0.029 μM. 27130357
L1210 Function assay 48 hrs Inhibitory concentration against proliferation of cultured lymphoid leukemia L1210 cells during 48 hrs, ID50 = 0.027 μM. 7131483
A549 Antiproliferative assay 48 hrs Antiproliferative activity against human A549 cells after 48 hrs by SRB assay, GI50 = 0.02 μM. 25827522
MDA-MB-231 Antiproliferative assay 48 hrs Antiproliferative activity against human MDA-MB-231 cells after 48 hrs by SRB assay, GI50 = 0.017 μM. 25827522
HeLa Antiproliferative assay 48 hrs Antiproliferative activity against human HeLa cells after 48 hrs by SRB assay, GI50 = 0.015 μM. 25827522
MDA-MB-231 Antiproliferative assay 48 hrs Antiproliferative activity against human MDA-MB-231 cells after 48 hrs by SRB cell proliferation assay, GI50 = 0.01 μM. 29089233
HeLa Antiproliferative assay 48 hrs Antiproliferative activity against human HeLa cells after 48 hrs by SRB cell proliferation assay, GI50 = 0.01 μM. 29089233
A549 Antiproliferative assay 48 hrs Antiproliferative activity against human A549 cells after 48 hrs by SRB cell proliferation assay, GI50 = 0.01 μM. 29089233
MDA-MB-231 Antiproliferative assay 48 hrs Antiproliferative activity against human MDA-MB-231 cells after 48 hrs by SRB assay, GI50 = 0.01 μM. 27688193
HeLa Antiproliferative assay 48 hrs Antiproliferative activity against human HeLa cells after 48 hrs by SRB assay, GI50 = 0.01 μM. 27688193
PANC1 Antiproliferative assay 48 hrs Antiproliferative activity against human PANC1 cells after 48 hrs by SRB assay, GI50 = 0.01 μM. 27688193
LT12 Antiproliferative assay 48 hrs Antiproliferative activity against rat LT12 cells after 48 hrs by XTT assay, IC50 = 0.006 μM. 19220018
L1210 Antiproliferative assay 48 hrs Antiproliferative activity against L1210 cells by XTT assay after 48 hrs, IC50 = 0.05 μM. 17181164
P388 Antiproliferative assay 48 hrs Antiproliferative activity against mouse adriamycin-resistant P388 cells after 48 hrs by XTT assay, IC50 = 0.05 μM. 17973361
L12 Antiproliferative assay 48 hrs Antiproliferative activity against rat multidrug-resistant L12 cells after 48 hrs by XTT assay, IC50 = 0.05 μM. 17973361
L1210 Antiproliferative assay 48 hrs Antiproliferative activity against mouse L1210 cells after 48 hrs by XTT assay, IC50 = 0.05 μM. 20537765
P388 Antiproliferative assay 48 hrs Antiproliferative activity against ADR-resistant mouse P388 cells after 48 hrs by XTT assay, IC50 = 0.05 μM. 21705223
U2OS Function assay 72 hrs Induction of mitotic arrest in human U2OS cells assessed as mitotic index after 72 hrs by microscopic analysis, EC50 = 0.05 μM. 24900887
L1210 Antiproliferative assay 48 hrs Antiproliferative activity against mouse L1210 cells after 48 hrs by XTT assay, IC50 = 0.06 μM. 17973361
L1210 Antiproliferative assay 48 hrs Antiproliferative activity against mouse L1210 cells after 48 hrs by XTT assay, IC50 = 0.06 μM. 19220018
L1210 Antiproliferative assay 48 hrs Antiproliferative activity against mouse L1210 cells after 48 hrs by XTT assay, IC50 = 0.06 μM. 21705223
HeLa Anticancer assay 48 hrs Anticancer activity against human HeLa cells after 48 hrs by SRB assay, GI50 = 0.063 μM. 27130357
MDA-MB-231 Cytotoxicity assay 48 hrs Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 48 hrs by SRB assay, GI50 = 0.065 μM. 28117204
NCI-H460 Antiproliferative assay 48 hrs Antiproliferative activity against human NCI-H460 cells after 48 hrs by alamar blue assay, IC50 = 0.068 μM. 21563750
BT549 Antiproliferative assay 48 hrs Antiproliferative activity against human BT549 cells after 48 hrs by alamar blue assay, IC50 = 0.069 μM. 21563750
LT12 MDR Antiproliferative assay 48 hrs Antiproliferative activity against LT12 MDR cells by XTT assay after 48 hrs, IC50 = 0.07 μM. 17181164
L1210 Antiproliferative assay 48 hrs Antiproliferative activity against mouse vincristine-resistant L1210 cells after 48 hrs by XTT assay, IC50 = 0.07 μM. 17973361
P388 Antiproliferative assay 48 hrs Antiproliferative activity against mouse P388 cells after 48 hrs by XTT assay, IC50 = 0.07 μM. 17973361
LT12 Antiproliferative assay 48 hrs Antiproliferative activity against multi drug-resistant rat LT12 cells after 48 hrs by XTT assay, IC50 = 0.07 μM. 20537765
P388 Antiproliferative assay 48 hrs Antiproliferative activity against mouse P388 cells after 48 hrs by XTT proliferation assay, IC50 = 0.07 μM. 21705223
LT12 Antiproliferative assay 48 hrs Antiproliferative activity against rat multiple drug-resistant LT12 cells after 48 hrs by XTT assay, IC50 = 0.07 μM. 21705223
L1210 Antiproliferative assay 48 hrs Antiproliferative activity against mouse VCR-resistant L1210 cells after 48 hrs by XTT assay, IC50 = 0.07 μM. 21705223
451LU Antiproliferative assay 48 hrs Antiproliferative activity against human 451LU cells after 48 hrs by alamar blue assay, IC50 = 0.072 μM. 21563750
P388 Antiproliferative assay 48 hrs Antiproliferative activity against P388 cells by XTT assay after 48 hrs, IC50 = 0.08 μM. 17181164
P388 Antiproliferative assay 48 hrs Antiproliferative activity against mouse P388 cells after 48 hrs by XTT assay, IC50 = 0.08 μM. 20537765
SKOV3 Antiproliferative assay 48 hrs Antiproliferative activity against human SKOV3 cells after 48 hrs by alamar blue assay, IC50 = 0.08 μM. 21563750
SGC7901 Growth inhibition assay 72 hrs Growth inhibition of human SGC7901 cells after 72 hrs by MTT assay, IC50 = 0.08 μM. 25262051
A549 Anticancer assay 48 hrs Anticancer activity against human A549 cells after 48 hrs by SRB assay, GI50 = 0.08 μM. 27130357
SKBR3 Growth inhibition assay 48 hrs Growth inhibition of human SKBR3 cells after 48 hrs by MTT assay, IC50 = 0.084 μM. 22850214
HeLa Cytotoxicity assay 48 hrs Cytotoxicity against human HeLa cells assessed as growth inhibition after 48 hrs by SRB assay, GI50 = 0.086 μM. 28117204
COLO205 Antiproliferative assay 48 hrs Antiproliferative activity against human COLO205 cells after 48 hrs by alamar blue assay, IC50 = 0.088 μM. 21563750
L1210 VCR Antiproliferative assay 48 hrs Antiproliferative activity against L1210 VCR cells by XTT assay after 48 hrs, IC50 = 0.09 μM. 17181164
L1210 Antiproliferative assay 48 hrs Antiproliferative activity against vincristine-resistant mouse L1210 cells after 48 hrs by XTT assay, IC50 = 0.09 μM. 20537765
A549 Cytotoxicity assay 48 hrs Cytotoxicity against human A549 cells assessed as growth inhibition after 48 hrs by SRB assay, GI50 = 0.09 μM. 28117204
KB/HeLa Cell cycle assay 24 hrs Cell cycle arrest in KB/HeLa cells assessed as accumulation at G2/M phase after 24 hrs by flow cytometric analysis, EC50 = 0.091 μM. 17973361
KB/HeLa Cell cycle assay 24 hrs Cell cycle arrest in human KB/HeLa cells assessed as G2/M phase accumulation after 24 hrs by FACS analysis, EC50 = 0.091 μM. 19220018
KB/HeLa Cell cycle assay 24 hrs Cell cycle arrest in human KB/HeLa cells assessed as accumulation at G2/M phase after 24 hrs using propidium iodide staining by FACS analysis, EC50 = 0.091 μM. 20537765
KB/HeLa Cell cycle assay 24 hrs Cell cycle arrest in human KB/HeLa cells assessed as accumulation of cells at G2/M phase after 24 hrs by flow cytometry, EC50 = 0.091 μM. 21705223
MiaPaCa Cytotoxicity assay 48 hrs Cytotoxicity against human MiaPaCa cells assessed as growth inhibition after 48 hrs by SRB assay, GI50 = 0.091 μM. 28117204
A549 Cell cycle assay 24 hrs Cell cycle arrest in human A549 cells assessed as accumulation at G2/M phase after 24 hrs using propidium iodide staining by flow cytometry, EC50 = 0.1065 μM. 20110137
RKO Antiproliferative assay 48 hrs Antiproliferative activity against RKO cells by XTT assay after 48 hrs, IC50 = 0.11 μM. 17181164
RKO Cytotoxicity assay 48 hrs Cytotoxicity against human RKO cells after 48 hrs by XTT assay, IC50 = 0.11 μM. 17973361
RKO Antiproliferative assay 48 hrs Antiproliferative activity against human RKO cells after 48 hrs by XTT assay, IC50 = 0.11 μM. 20537765
RKOp27 Cytotoxicity assay 48 hrs Cytotoxicity against non induced human RKOp27 cells after 48 hrs by XTT assay, IC50 = 0.11 μM. 21705223
SW480 Antiproliferative assay 48 hrs Antiproliferative activity against human SW480 cells after 48 hrs by alamar blue assay, IC50 = 0.12 μM. 21563750
A549 Growth inhibition assay 72 hrs Growth inhibition of human A549 cells after 72 hrs by MTT assay, IC50 = 0.12 μM. 25262051
KB/HeLa Antiproliferative assay 48 hrs Antiproliferative activity against KB/HeLa cells by XTT assay after 48 hrs, IC50 = 0.14 μM. 17181164
KB/HeLa Cytotoxicity assay 48 hrs Cytotoxicity against human KB/HeLa cells after 48 hrs by XTT assay, IC50 = 0.14 μM. 17973361
KB/HeLa Antiproliferative assay 48 hrs Antiproliferative activity against human KB/HeLa cells after 48 hrs by XTT assay, IC50 = 0.14 μM. 20537765
KB/HeLa Cytotoxicity assay 48 hrs Cytotoxicity against human KB/HeLa cells after 48 hrs by XTT assay, IC50 = 0.14 μM. 21705223
HT1080 Growth inhibition assay 72 hrs Growth inhibition of human HT1080 cells after 72 hrs by MTT assay, IC50 = 0.14 μM. 25262051
NCI-H460 Antiproliferative assay 48 hrs Antiproliferative activity against NCI-H460 cells by XTT assay after 48 hrs, IC50 = 0.15 μM. 17181164
NCI-H460 Cytotoxicity assay 48 hrs Cytotoxicity against human NCI-H460 cells after 48 hrs by XTT assay, IC50 = 0.15 μM. 17973361
NCI-H460 Antiproliferative assay 48 hrs Antiproliferative activity against human NCI-H460 cells after 48 hrs by XTT assay, IC50 = 0.15 μM. 20537765
NCI-H460 Cytotoxicity assay 48 hrs Cytotoxicity against human NCI-H460 cells after 48 hrs by XTT assay, IC50 = 0.15 μM. 21705223
SKOV3 Antiproliferative assay 48 hrs Antiproliferative activity against SKOV3 cells by XTT assay after 48 hrs, IC50 = 0.17 μM. 17181164
P388 ADR Antiproliferative assay 48 hrs Antiproliferative activity against P388 ADR cells by XTT assay after 48 hrs, IC50 = 0.17 μM. 17181164
SKOV3 Cytotoxicity assay 48 hrs Cytotoxicity against human SKOV3 cells after 48 hrs by XTT assay, IC50 = 0.17 μM. 17973361
SKOV3 Antiproliferative assay 48 hrs Antiproliferative activity against human SKOV3 cells after 48 hrs by XTT assay, IC50 = 0.17 μM. 20537765
P388 Antiproliferative assay 48 hrs Antiproliferative activity against adriamycin-resistant mouse P388 cells after 48 hrs by XTT assay, IC50 = 0.17 μM. 20537765
SKOV3 Cytotoxicity assay 48 hrs Cytotoxicity against human SKOV3 cells after 48 hrs by XTT assay, IC50 = 0.17 μM. 21705223
HeLa Cytotoxicity assay 24 to 48 hrs Cytotoxicity against human HeLa cells after 24 to 48 hrs by trypan blue assay, IC50 = 0.178 μM. 23445405
DLD1 Antiproliferative assay 48 hrs Antiproliferative activity against human DLD1 cells after 48 hrs by alamar blue assay, IC50 = 0.19 μM. 21563750
SF268 Antiproliferative assay 48 hrs Antiproliferative activity against SF268 cells by XTT assay after 48 hrs, IC50 = 0.3 μM. 17181164
SF268 Cytotoxicity assay 48 hrs Cytotoxicity against human SF268 cells after 48 hrs by XTT assay, IC50 = 0.3 μM. 17973361
L12 Antiproliferative assay 48 hrs Antiproliferative activity against rat L12 cells after 48 hrs by XTT assay, IC50 = 0.3 μM. 17973361
SF268 Antiproliferative assay 48 hrs Antiproliferative activity against human SF268 cells after 48 hrs by XTT assay, IC50 = 0.3 μM. 20537765
SF268 Cytotoxicity assay 48 hrs Cytotoxicity against human SF268 cells after 48 hrs by XTT assay, IC50 = 0.3 μM. 21705223
LT12 Antiproliferative assay 48 hrs Antiproliferative activity against rat LT12 cells ectopic expression of human MDR1 after 48 hrs by XTT assay, IC50 = 0.4 μM. 19220018
A549 Cytotoxicity assay 48 hrs Cytotoxicity against human A549 cells after 48 hrs by SRB assay, GI50 = 0.5 μM. 20529689
SK-N-SH Cytotoxicity assay 48 hrs Cytotoxicity against human SK-N-SH cells after 48 hrs by SRB assay, GI50 = 0.65 μM. 20529689
HeLa Cytotoxicity assay 48 hrs Cytotoxicity against human HeLa cells after 48 hrs by MTT assay, IC50 = 0.71 μM. 23748152
HeLa Antiproliferative assay 48 hrs Antiproliferative activity against human HeLa cells assessed as growth inhibition after 48 hrs by SRB assay, GI50 = 0.81 μM. 25131956
HeLa Antiproliferative assay 48 hrs Antiproliferative activity against human HeLa cells after 48 hrs by SRB assay, GI50 = 0.81 μM. 27344493
A549 Cytotoxicity assay 48 hrs Cytotoxicity against human A549 cells assessed as cell viability after 48 hrs by MTT assay, IC50 = 0.89 μM. 25599948
A549 Antiproliferative assay 48 hrs Antiproliferative activity against human A549 cells after 48 hrs by SRB assay, IC50 = 0.9 μM. 28988624
MDA-MB-453 Cytotoxicity assay 48 hrs Cytotoxicity in human MDA-MB-453 cells incubated for 48 hrs by MTT assay, IC50 = 0.91 μM. 28923381
MCF7 Antiproliferative assay 48 hrs Antiproliferative activity against human MCF7 cells assessed as growth inhibition after 48 hrs by SRB assay, GI50 = 0.94 μM. 25131956
MCF7 Antiproliferative assay 48 hrs Antiproliferative activity against human MCF7 cells after 48 hrs by SRB assay, GI50 = 0.94 μM. 27344493
MIAPaCa2 Antiproliferative assay 48 hrs Antiproliferative activity against human MIAPaCa2 cells assessed as growth inhibition after 48 hrs by SRB assay, GI50 = 0.95 μM. 25131956
MIAPaCa2 Antiproliferative assay 48 hrs Antiproliferative activity against human MIAPaCa2 cells after 48 hrs by SRB assay, GI50 = 0.95 μM. 27344493
A549 Cytotoxicity assay 48 hrs Cytotoxicity against human A549 cells after 48 hrs by SRB assay, LC50 = 1 μM. 20529689
HCT116 Cytotoxicity assay 48 hrs Cytotoxicity in human HCT116 cells incubated for 48 hrs by MTT assay, IC50 = 1.06 μM. 28923381
HeLa Cytotoxicity assay 48 hrs Cytotoxicity against human HeLa cells assessed as cell viability after 48 hrs by MTT assay, IC50 = 1.1 μM. 25599948
DU145 Cytotoxicity assay 48 hrs Cytotoxicity in human DU145 cells incubated for 48 hrs by MTT assay, IC50 = 1.14 μM. 28923381
HeLa Antiproliferative assay 48 hrs Antiproliferative activity against human HeLa cells after 48 hrs by SRB assay, IC50 = 1.2 μM. 28988624
MCF7 Antiproliferative assay 48 hrs Antiproliferative activity against human MCF7 cells after 48 hrs by SRB assay, IC50 = 1.25 μM. 28988624
HCT116 Anticancer assay 48 hrs Anticancer activity against human HCT116 cells after 48 hrs by MTT assay, IC50 = 1.262 μM. 28789894
IMR32 Antiproliferative assay 48 hrs Antiproliferative activity against human IMR32 cells after 48 hrs by SRB assay, IC50 = 1.3 μM. 28988624
A549 Anticancer assay 48 hrs Anticancer activity against human A549 cells after 48 hrs by MTT assay, IC50 = 1.513 μM. 28789894
MCF7 Anticancer assay 48 hrs Anticancer activity against human MCF7 cells assessed as reduction in cell growth incubated for 48 hrs by MTT assay, IC50 = 1.6 μM. 26231080
DU145 Anticancer assay 48 hrs Anticancer activity against human DU145 cells after 48 hrs by MTT assay, IC50 = 1.698 μM. 28789894
MCF7 Cytotoxicity assay 48 hrs Cytotoxicity against human MCF7 cells assessed as cell viability after 48 hrs by MTT assay, IC50 = 1.7 μM. 25599948
PC3 Cytotoxicity assay 48 hrs Cytotoxicity in human PC3 cells incubated for 48 hrs by MTT assay, IC50 = 1.84 μM. 28923381
MDA-MB-231 Anticancer assay 48 hrs Anticancer activity against human MDA-MB-231 cells after 48 hrs by MTT assay, IC50 = 1.862 μM. 28789894
A549 Anticancer assay 48 hrs Anticancer activity against human A549 cells assessed as reduction in cell growth incubated for 48 hrs by MTT assay, IC50 = 1.87 μM. 26231080
A549 Cytotoxicity assay 48 hrs Cytotoxicity against human A549 cells assessed as cell growth inhibition after 48 hrs by MTT assay, IC50 = 1.87 μM. 28668476
A549 Cytotoxicity assay 48 hrs Cytotoxicity against human A549 cells after 48 hrs by MTT assay, IC50 = 2.31 μM. 23748152
SK-N-SH Cytotoxicity assay 48 hrs Cytotoxicity against human SK-N-SH cells after 48 hrs by SRB assay, LC50 = 2.8 μM. 20529689
DU145 Cytotoxicity assay 48 hrs Cytotoxicity against human DU145 cells after 48 hrs by MTT assay, IC50 = 2.8 μM. 23748152
HeLa Anticancer assay 48 hrs Anticancer activity against human HeLa cells assessed as reduction in cell growth incubated for 48 hrs by MTT assay, IC50 = 2.83 μM. 26231080
HeLa Cytotoxicity assay 48 hrs Cytotoxicity against human HeLa cells assessed as cell growth inhibition after 48 hrs by MTT assay, IC50 = 2.83 μM. 28668476
DU145 Cytotoxicity assay 48 hrs Cytotoxicity against human DU145 cells assessed as cell viability after 48 hrs by MTT assay, IC50 = 3.2 μM. 25599948
HaCaT Cytotoxicity assay 48 hrs Cytotoxic activity against human HaCaT cells assessed as reduction in cell growth incubated for 48 hrs by MTT assay, IC50 = 8.9 μM. 26231080
A549 Cell cycle assay 24 hrs Cell cycle arrest in human A549 cells assessed as accumulation of cells in G2 phase after 24 hrs measured using propidium iodide staining by FACS analysis 21863853
Jurkat T Function assay 36 hrs Induction of microtubule damage in human Jurkat T cells overexpressing Bcl2 after 36 hrs by immunostaining method 22197393
Jurkat T Function assay 36 hrs Induction of microtubule damage in human Jurkat T cells overexpressing Neo after 36 hrs by immunostaining method 22197393
Jurkat T Apoptosis assay 36 hrs Induction of apoptosis in human Jurkat T cells overexpressing Bcl2 assessed as cellular protrusions after 36 hrs by light microscopy 22197393
Jurkat T Apoptosis assay 36 hrs Induction of apoptosis in human Jurkat T cells overexpressing Neo after 36 hrs by flow cytometry 22197393
Jurkat T Apoptosis assay 36 hrs Induction of apoptosis in human Jurkat T cells overexpressing Neo assessed as cellular protrusions after 36 hrs by light microscopy 22197393
SGC7901 Function assay 48 hrs Induction of distortions in tubulin assembly in human SGC7901 cells assessed as abnormal mitotic spindle formation at 2 fold IC50 level incubated for 48 hrs by immunofluorescence staining based fluorescence microscopy 25262051
HT1080 Function assay 48 hrs Induction of distortions in tubulin assembly in human HT1080 cells assessed as abnormal mitotic spindle formation at 2 fold IC50 level incubated for 48 hrs by immunofluorescence staining based fluorescence microscopy 25262051
HeLa Function assay 24 hrs Inhibition of tubulin polymerization in human HeLa cells assessed as increase in soluble tubulin fraction after 24 hrs by Western blot analysis 28818768
L1210 murine leukemia cell Growth inhibition assay In vitro for its inhibitory activity against L1210 murine leukemia cell growth, IC50=38 nM 1738146
K562 Function assay Effective concentration required to arrest propidium iodide stained K562 cells in G2/M phase, EC50 = 0.082 μM. 12852768
A549 Cytotoxicity assay Cytotoxicity against human A549 cells by MTT assay, IC50 = 0.087 μM. 21954851
KB/HeLa Cell cycle assay Cell cycle arrest in KB/HeLa cells by accumulation at G2/M phase, EC50 = 0.091 μM. 17181164
MCF7 Growth inhibition assay Growth inhibition of human MCF7 cells by Sulforhodamine B assay, GI50 = 0.1 μM. 21106458
MCF7 Growth inhibition assay Growth inhibition of human MCF7 cells by Sulforhodamine B assay, LC50 = 0.1 μM. 21106458
A549 Growth inhibition assay Growth inhibition of human A549 cells by Sulforhodamine B assay, GI50 = 0.1 μM. 21106458
A549 Growth inhibition assay Growth inhibition of human A549 cells by Sulforhodamine B assay, LC50 = 0.1 μM. 21106458
RKO Cytotoxicity assay Cytotoxic activity against human colon adenocarcinoma RKO cells without ectopic inducible expression of cyclin-dependent kinase inhibitor p27kip1, IC50 = 0.11 μM. 12852768
RKO Antiproliferative assay Antiproliferative activity against human RKO cells by XTT assay, IC50 = 0.11 μM. 19220018
KB/HeLa Antiproliferative assay Antiproliferative activity against human KB/HeLa cells by XTT assay, IC50 = 0.14 μM. 19220018
NCI-H460 Antiproliferative assay Antiproliferative activity against human NCI-H460 cells by XTT assay, IC50 = 0.15 μM. 19220018
SKOV3 Antiproliferative assay Antiproliferative activity against human SKOV3 cells by XTT assay, IC50 = 0.17 μM. 19220018
HCT116 Cytotoxicity assay Cytotoxicity against human HCT116 cells by SRB assay, IC50 = 0.173 μM. 21954851
SF268 Antiproliferative assay Antiproliferative activity against human SF268 cells by XTT assay, IC50 = 0.3 μM. 19220018
SK-N-SH Growth inhibition assay Growth inhibition of human SK-N-SH cells by Sulforhodamine B assay, GI50 = 0.66 μM. 21106458
HeLa Growth inhibition assay Growth inhibition of human HeLa cells by Sulforhodamine B assay, GI50 = 0.75 μM. 21106458
SK-N-SH Growth inhibition assay Growth inhibition of human SK-N-SH cells by Sulforhodamine B assay, LC50 = 0.75 μM. 21106458
K562 Function assay Inhibition of tubulin polymerization in human K562 cells, IC50 = 0.76 μM. 20546980
HeLa Growth inhibition assay Growth inhibition of human HeLa cells by Sulforhodamine B assay, LC50 = 0.9 μM. 21106458
TC32 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for TC32 cells 29435139
DAOY qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for DAOY cells 29435139
SJ-GBM2 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SJ-GBM2 cells 29435139
A673 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for A673 cells 29435139
SK-N-MC qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SK-N-MC cells 29435139
BT-37 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for BT-37 cells 29435139
NB-EBc1 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for NB-EBc1 cells 29435139
Saos-2 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for Saos-2 cells 29435139
NB1643 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for NB1643 cells 29435139
LAN-5 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for LAN-5 cells 29435139
BT-12 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for BT-12 cells 29435139
OHS-50 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for OHS-50 cells 29435139
NB1643 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for NB1643 cells 29435139
A673 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for A673 cells) 29435139
SK-N-MC qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for SK-N-MC cells 29435139
BT-12 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for BT-12 cells 29435139
LAN-5 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for LAN-5 cells 29435139
DAOY qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for DAOY cells 29435139
BT-37 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for BT-37 cells 29435139
TC32 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for TC32 cells 29435139
MG 63 (6-TG R) qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for MG 63 (6-TG R) cells 29435139
U-2 OS qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for U-2 OS cells 29435139
RD qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for RD cells 29435139
Saos-2 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for Saos-2 cells 29435139
OHS-50 qHTS assay qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for OHS-50 cells 29435139
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生物活性

產(chǎn)品描述 Nocodazole是一種微管聚合的快速可逆抑制劑，也抑制Abl、Abl(E255K)和Abl(T315I)，對應的IC50值分別為0.21 μM，0.53 μM和0.64 μM。Nocodazole可提高CRISPR介導的同源重組效率，對增強精確的基因編輯效力具有加成作用。Nocodazole可誘導凋亡。
靶點
Microtubules [2]
(Cell-free assay)		 Abl [1]
(Cell-free assay)		 Abl (E255K) [1]
(Cell-free assay)		 Abl (T315I) [1]
(Cell-free assay)
0.21 μM 0.53 μM 0.64 μM
體外研究(In Vitro)
體外研究活性

Nocodazole是癌癥相關激酶的高親和力配體，如的磷酸化ABL, c-KIT, BRAF, 和 MEK ，Kd值分別為0.091 μM, 1.6 μM, 1.8 μM 和1.6 μM,。此外，Nocodazole對ABL(E255K)磷酸化, ABL(T315I) 磷酸化, BRAF(V600E) 和 PI3Kγ 的Kd值分別是0.12 μM, 0.17 μM, 1.1 μM 和1.5 μM。Nocodazole誘導慢性淋巴細胞白血病細胞凋亡；抑制胰島素刺激的葡萄糖轉運；在人類結腸癌細胞減少細胞凋亡；損害內側神經(jīng)節(jié)嵴細胞的形態(tài)和方向遷移[1]。

Nocodazole于高濃度時，在細胞中迅速解聚微管，而低濃度可抑制微管的動態(tài)不穩(wěn)定[2]。

用Nocodazole處理經(jīng)同步化6小時的分裂期細胞，加入不同濃度的紫杉醇后，可造成G1期阻滯，半數(shù)抑制濃度為4nM。紫杉醇處理經(jīng)Nocodazole預處理的細胞，在無Nocodazole的條件下，細胞僅能形成游離微管，而在有Nocodazole存在的條件下，則可形成聚集的中心體微管[3]。

Nocodazole通過結合β-微管蛋白破壞微管，可阻礙二硫鍵形成和囊泡運輸，抑制METH誘導的細胞凋亡和溶酶體功能障礙。Nocodazole預處理可顯著減少METH誘導的細胞凋亡[4]。

在iPSC中，Nocodazole可使HDR效率加倍，可高達30%[6]。
實驗圖片 檢測方法 檢測指標 實驗圖片 PMID
Western blot Cdc2 / cyclin B1 / MAD2 / Cdc20 21918689
Immunofluorescence Tubulin / LC8 Brd4 / α-tubulin 23038268
Growth inhibition assay Cell viability 21918689
體內研究(In Vivo)
體內研究活性

Nocodazole聯(lián)合使用比單獨使用可更強地抑制小鼠的腫瘤體積和腫瘤重量。在COLO205腫瘤移植模型中聯(lián)合使用的誘導凋亡效果也比單獨使用更明顯[5]。
動物實驗 Animal Models COLO-205腫瘤移植裸鼠
Dosages 5 mg/kg
Administration 腹腔注射

化學信息&溶解度

分子量 301.32 分子式

C14H11N3O3S
CAS號 31430-18-9 SDF Download Nocodazole SDF
Smiles COC(=O)NC1=NC2=C(N1)C=C(C=C2)C(=O)C3=CC=CS3
儲存條件(自收到貨起)

體外溶解度
批次：

DMSO : 7.5 mg/mL ( (24.89 mM) ；DMSO吸濕會降低化合物溶解度，請使用新開封DMSO)

Water : Insoluble

Ethanol : Insoluble

摩爾濃度計算器

體內溶解度
批次：

現(xiàn)配現(xiàn)用，請按從左到右的順序依次添加，澄清后再加入下一溶劑

 動物體內配方計算器

實驗計算

摩爾濃度計算器

質量 濃度 體積 分子量

動物體內配方計算器（澄清溶液）

第一步：請輸入基本實驗信息（考慮到實驗過程中的損耗，建議多配一只動物的藥量）

mg/kg g μL 只

第二步：請輸入動物體內配方組成（配方適用于不溶于水的藥物；不同批次藥物配方比例不同，請聯(lián)系Selleck為您提供正確的澄清溶液配方）

% DMSO % % Tween 80 % ddH2O
%DMSO %

計算結果：

工作液濃度： mg/ml；

DMSO母液配制方法： mg 藥物溶于μL DMSO溶液（母液濃度mg/mL，注：如該濃度超過該批次藥物DMSO溶解度，請先聯(lián)系Selleck）；

體內配方配制方法：取μL DMSO母液，加入μL PEG300，混勻澄清后加入μL Tween 80，混勻澄清后加入μL ddH2O，混勻澄清。

體內配方配制方法：取μL DMSO母液，加入μL Corn oil，混勻澄清。

注意：1. 首先保證母液是澄清的；
2.一定要按照順序依次將溶劑加入，進行下一步操作之前必須保證上一步操作得到的是澄清的溶液，可采用渦旋、超聲或水浴加熱等物理方法助溶。

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