Vinblastine sulfate

別名: NSC-49842, Vincaleukoblastine, 29060-LE 中文名稱：硫酸長春堿

目錄號：S4505 Purity: 99.91%

Vinblastine sulfate抑制微管形成和nAChR活性，在無細胞實驗的測定中，IC50為8.9 μM。Vinblastine sulfate 可誘導自噬和凋亡。

Vinblastine sulfate Chemical Structure

CAS: 143-67-9

規(guī)格	價格	庫存
10mM (1mL in DMSO)	794.43	現(xiàn)貨
5mg	573.88	現(xiàn)貨
25mg	2023.45	現(xiàn)貨
100mg	5545.08	現(xiàn)貨
1g	20230.26	現(xiàn)貨
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產(chǎn)品質控

批次：純度： 99.91%

99.91

Vinblastine sulfate相關產(chǎn)品

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細胞實驗數(shù)據(jù)示例

細胞系	實驗類型	給藥濃度	孵育時間	活性描述	文獻信息
rat REF52 cells	Function assay	0.1 μM	30 mins	Induction of microtubule depolymerization in rat REF52 cells at 0.1 uM after 30 mins by fluorescence assay	23947826
K562 cell	Proliferation assay		48 h	Antiproliferative activity against human K562 cells after 48 hrs, IC50=0.001 μM	19220018
ACHN cells	Cytotoxicity assay		48 h	Cytotoxicity against human ACHN cells after 48 hrs by SRB assay, IC50=22.7 μM	19467877
A375 cells	Cytotoxicity assay		48 h	Cytotoxicity against human A375 cells after 48 hrs by SRB assay, IC50=7.2 μM	19467877
C32 cells	Cytotoxicity assay		48 h	Cytotoxicity against human C32 cells after 48 hrs by SRB assay, IC50=3 μM	19467877
LNCAP cells	Cytotoxicity assay		48 h	Cytotoxicity against human LNCAP cells after 48 hrs by SRB assay, IC50=29.3 μM	19467877
Huh-7D12 cells	Cytotoxicity assay		48 h	Cytotoxicity against human Huh-7D12 cells after 48 hrs by SRB assay, IC50=45.6 μM	19467877
COR-L23 cells	Cytotoxicity assay		48 h	Cytotoxicity against human COR-L23 cells after 48 hrs by SRB assay, IC50=45.5 μM	19467877
142BR cells	Cytotoxicity assay		48 h	Cytotoxicity against human 142BR cells after 48 hrs by SRB assay, IC50=37.6 μM	19467877
HT-29 cells	Cytotoxicity assay		48 h	Cytotoxicity against human HT-29 cells after 48 hrs by MTS assay, IC50=0.55 μM	21920762
A549 cells	Proliferation assay		48 h	Antiproliferative activity against human A549 cells after 48 hrs by MTS assay, IC50=2.36 μM	22546674
DU145 cells	Proliferation assay		48 h	Antiproliferative activity against human DU145 cells after 48 hrs by MTS assay, IC50=4.25 μM	22546674
SK-MEL-5 cells	Proliferation assay		48 h	Antiproliferative activity against human SK-MEL-5 cells after 48 hrs by MTS assay, IC50=1.74 μM	22546674
HepG2 cells	Proliferation assay		48 h	Antiproliferative activity against human HepG2 cells after 48 hrs by MTS assay, IC50=0.16 μM	22546674
HT-29 cells	Proliferation assay		48 h	Antiproliferative activity against human HT-29 cells after 48 hrs by MTS assay, IC50=11.18 μM	22546674
MCF7 cells	Proliferation assay		48 h	Antiproliferative activity against human MCF7 cells after 48 hrs by MTS assay, IC50=24.08 μM	22546674
MDA-MB-231 cells	Proliferation assay		48 h	Antiproliferative activity against human MDA-MB-231 cells after 48 hrs by MTS assay, IC50=31.52 μM	22546674
HepG2 cells	Cytotoxicity assay		72 h	Cytotoxicity against adriamycin-resistant human HepG2 cells assessed as growth inhibition after 72 hrs by MTT assay, IC50=0.056 μM	23708010
HepG2 cells	Cytotoxicity assay		72 h	Cytotoxicity against human HepG2 cells assessed as growth inhibition after 72 hrs by MTT assay, IC50=0.019 μM	23708010
K562 cells	Cytotoxicity assay		72 h	Cytotoxicity against human K562 cells assessed as growth inhibition after 72 hrs by MTT assay, IC50=0.016 μM	23708010
MDA-MB-231 cells	Cytotoxicity assay		72 h	Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 72 hrs by MTT assay, IC50=0.0083 μM	23708010
MCF7 cells	Cytotoxicity assay		72 h	Cytotoxicity against human MCF7 cells assessed as growth inhibition after 72 hrs by MTT assay, IC50=0.007 μM	23708010
UACC903 cells	Cytotoxicity assay		48 h	Cytotoxicity against human UACC903 cells after 48 hrs by MTS assay, IC50=1.65 μM	21920762
BxPC3 cells	Proliferation assay		48 h	Antiproliferative activity against human BxPC3 cells after 48 hrs by MTS assay, IC50=1.13 μM	22546674
COLO 320 human colorectal carcinoma cell line	Function assay			In vitro concentration required to kill 50% of COLO 320 human colorectal carcinoma cell line, EC50=0.08 μM	12361397
LNCaP human prostate cancer cell line	Function assay			In vitro concentration required to kill 50% of LNCaP human prostate cancer cell line, EC50=0.5 μM	12361397
K562 cell	Growth inhibition assay			In vitro inhibitory concentration against human chronic myelogenous leukemia K562 cell growth, IC50=0.001 μM	12852768
T47D cells	Function assay			In vitro concentration required to kill 50% of T47D human breast ductal carcinoma cell line, EC50=0.08 μM	12361397
SCL6 cells	Cytotoxicity assay			Cytotoxicity against human SCL6 cells by MTT assay, ED50=6.1 Μm	12880314
SCL9	Cytotoxicity assay			Cytotoxicity against human SCL9 cells by MTT assay, ED=5.3 μM	12880314
KATO III cells	Cytotoxicity assay			Cytotoxicity against human KATO III cells by MTT assay, ED50=6.1 μM	12880314
NUGC4 cells	Cytotoxicity assay			Cytotoxicity against human NUGC4 cells by MTT assay, ED50=5.3 μM	12880314
K562 cells	Function assay			Inhibition of tubulin polymerization in human K562 cells, IC50=0.13 μM	20546980
點擊查看更多細胞系數(shù)據(jù)

生物活性

產(chǎn)品描述

Vinblastine sulfate抑制微管形成和nAChR活性，在無細胞實驗的測定中，IC50為8.9 μM。Vinblastine sulfate 可誘導自噬和凋亡。

靶點

nAChR ^[1]
(Adrenal Chromaffin Cells)

8.9 μM

體外研究(In Vitro)
體外研究活性	Vinblastine的平均終末半衰期為14.3小時。Vinblastine加入到新鮮分離的大鼠肝細胞，可迅速穿透到細胞當中^[3]。Vinblastine抑制由腎上腺髓質素誘導的血管生成反應，還可引起M期阻滯^[4]。在特定濃度下，vinblastine能顯著增加微核單核細胞的數(shù)量^[2]。
細胞實驗	細胞系	中國倉鼠卵巢細胞(CHO)
	濃度	1% (v/v) (dissolved in DMSO)
	孵育時間	3 h
	方法	6孔細胞培養(yǎng)板，每孔細胞密度為5×10⁴ cells/mL，懸浮于3 mL培養(yǎng)基中。向其中加入vinblastine孵育3小時，生長21小時后進行后續(xù)檢測。
實驗圖片	檢測方法	檢測指標	實驗圖片	PMID
	Western blot	ERK / p-ERK / Mcl-1 / Bad / Bid / Noxa p-JNK / c-Caspase-7 / c-PARP p-eIF2 GRP78		20371726
	Immunofluorescence	α-tubulin / Acetyl tubulin		30120268
	Growth inhibition assay	Cell viability		27114800

體內研究(In Vivo)
體內研究活性	Vinblastine作為抗癌劑被廣泛運用，但具有一些意外的副作用^[6]。Vinblastine和RAP在低濃度下的結合使用，可在體內得到比較滿意的抗血管形成作用^[4]。在臨床使用劑量下，vinblastine抑制CEM細胞中的微管蛋白的棕櫚?；?sup>[5]。

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
臨床試驗信息 (data from https://clinicaltrials.gov, updated on 2024-05-22)
NCT06381570	Recruiting	Low-grade Glioma	Daniel Morgenstern\|The Hospital for Sick Children	March 21 2024	Early Phase 1

參考文獻
[1] McKay DB, et al. Proc Soc Exp Biol Med. 1993, 203(3):372-376. [2] George E. Johnson, et al. Mutat Res. 2010, 702(2):189-192. [3] Zhou XJ, et al. Eur J Drug Metab Pharmacokinet. 1990, 15(4):323-332. [4] Ribatti D, et al. Oncogene. 2003, 22(41):6458-6461. [5] Joan M. Caron, et al. Chemotherapy. 2007, 53:51-58. + 展開

參考文獻

[1] McKay DB, et al. Proc Soc Exp Biol Med. 1993, 203(3):372-376.
[2] George E. Johnson, et al. Mutat Res. 2010, 702(2):189-192.
[3] Zhou XJ, et al. Eur J Drug Metab Pharmacokinet. 1990, 15(4):323-332.

[4] Ribatti D, et al. Oncogene. 2003, 22(41):6458-6461.
[5] Joan M. Caron, et al. Chemotherapy. 2007, 53:51-58.

+ 展開

化學信息&溶解度

分子量	909.05	分子式	C₄₆H₅₈N₄O₉.H₂SO₄
CAS號	143-67-9	SDF	Download Vinblastine sulfate SDF
Smiles	CCC1(CC2CC(C3=C(CCN(C2)C1)C4=CC=CC=C4N3)(C5=C(C=C6C(=C5)C78CCN9C7C(C=CC9)(C(C(C8N6C)(C(=O)OC)O)OC(=O)C)CC)OC)C(=O)OC)O.OS(=O)(=O)O
儲存條件(自收到貨起)	3年-20°C粉狀	儲備液保存和期限建議分裝儲備液，避免反復凍融！	1年-80°C溶于溶劑
儲存條件(自收到貨起)	3年-20°C粉狀	儲備液保存和期限建議分裝儲備液，避免反復凍融！	1個月-20°C溶于溶劑

體外溶解度
批次：

DMSO : 100 mg/mL ( (110.0 mM) ；DMSO吸濕會降低化合物溶解度，請使用新開封DMSO)

Water : 50 mg/mL (55.0 mM)

Ethanol : Insoluble

DMSO : 100 mg/mL ( (110.0 mM) ；DMSO吸濕會降低化合物溶解度，請使用新開封DMSO)

Water : Insoluble

Ethanol : Insoluble

摩爾濃度計算器

體內溶解度
批次：

現(xiàn)配現(xiàn)用，請按從左到右的順序依次添加，澄清后再加入下一溶劑

動物體內配方計算器

實驗計算

摩爾濃度計算器

質量	濃度	體積	分子量
=	×	×

稀釋計算器分子量計算器

動物體內配方計算器（澄清溶液）

第一步：請輸入基本實驗信息（考慮到實驗過程中的損耗，建議多配一只動物的藥量）

給藥劑量 mg/kg 動物平均體重 g 每只動物給藥體積 μL 動物數(shù)量只

第二步：請輸入動物體內配方組成（配方適用于不溶于水的藥物；不同批次藥物配方比例不同，請聯(lián)系Selleck為您提供正確的澄清溶液配方）

% DMSO + % + % Tween 80 + % ddH2O

%DMSO+ %

計算結果：

工作液濃度： mg/ml；

DMSO母液配制方法： mg 藥物溶于μL DMSO溶液（母液濃度mg/mL，注：如該濃度超過該批次藥物DMSO溶解度，請先聯(lián)系Selleck）；

體內配方配制方法：取μL DMSO母液，加入μL PEG300，混勻澄清后加入μL Tween 80，混勻澄清后加入μL ddH₂O，混勻澄清。

體內配方配制方法：取μL DMSO母液，加入μL Corn oil，混勻澄清。

注意：1. 首先保證母液是澄清的；
2.一定要按照順序依次將溶劑加入，進行下一步操作之前必須保證上一步操作得到的是澄清的溶液，可采用渦旋、超聲或水浴加熱等物理方法助溶。

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