- 抑制劑
- 化合物庫
- 抗體
- 生物試劑
- qPCR
- 2x SYBR Green qPCR Master Mix
- 2x SYBR Green qPCR Master Mix(Low ROX)
- 2x SYBR Green qPCR Master Mix(High ROX)
- 蛋白實(shí)驗(yàn)
- Protein A/G免疫沉淀磁珠
- Anti-DYKDDDDK Tag免疫磁珠
- Anti-DYKDDDDK Tag親和凝膠
- Anti-Myc免疫磁珠
- Anti-HA免疫磁珠
- 磁力架
- Poly DYKDDDDK Tag多肽
- 蛋白酶抑制劑Cocktail
- 蛋白酶抑制劑Cocktail(DMSO儲液)
- 磷酸酶抑制劑Cocktail
- 細(xì)胞實(shí)驗(yàn)
- Cell Counting Kit-8 (CCK-8)
- 新產(chǎn)品
- 聯(lián)系我們
Torin 1
Torin 1是一種有效的mTORC1/2抑制劑，在無細(xì)胞試驗(yàn)中IC50為2 nM/10 nM；作用于mTOR比作用于PI3K選擇性高1000倍。
Torin 1 Chemical Structure
CAS: 1222998-36-8
產(chǎn)品質(zhì)控
批次：
純度：
99.07%
99.07
常與Torin 1一起在實(shí)驗(yàn)中被使用的化合物
Torin 1和Rapamycin差異調(diào)節(jié)人神經(jīng)母細(xì)胞瘤細(xì)胞中鐵誘導(dǎo)的線粒體ROS的產(chǎn)生。
Torin 1和Verteporfin聯(lián)合治療在PDX模型中產(chǎn)生最大程度的持續(xù)腫瘤生長抑制。
Torin 1和Chloroquine降低BON1細(xì)胞的細(xì)胞活力并抑制細(xì)胞增殖。
Avneil-Polak S, et al. Neuroendocrinology. 2016;103(6):724-37.
Torin 1可使EGFR野生型、KRAS或NRAS、p53野生型或突變型的NSCLC細(xì)胞系對Erlotinib的敏感性提高10-20倍。
Torin 1和Cetuximab對OSCC異種移植小鼠的腫瘤體積生長有抑制作用。
Torin 1相關(guān)產(chǎn)品
| 相關(guān)靶點(diǎn) | mTORC1 mTORC2 | 點(diǎn)擊展開 |
|---|---|---|
| 相關(guān)產(chǎn)品 | AZD8055 Ridaforolimus (Deforolimus, MK-8669) Sapanisertib (MLN0128) Torkinib (PP242) Vistusertib (AZD2014) MHY1485 KU-0063794 Torin 2 OSI-027 WYE-354 WYE-125132 (WYE-132) 3BDO Palomid 529 (P529) Zotarolimus (ABT-578) Salidroside GDC-0349 WAY-600 Onatasertib (CC 223) XL388 WYE-687 | 點(diǎn)擊展開 |
| 相關(guān)化合物庫 | 激酶抑制劑庫 PI3K/Akt 抑制劑庫 凋亡分子化合物庫 細(xì)胞周期化合物庫 NF-κB信號通路庫 | 點(diǎn)擊展開 |
相關(guān)信號通路圖
細(xì)胞實(shí)驗(yàn)數(shù)據(jù)示例
| 細(xì)胞系 | 實(shí)驗(yàn)類型 | 給藥濃度 | 孵育時(shí)間 | 活性描述 | 文獻(xiàn)信息 |
|---|---|---|---|---|---|
| RPMI-8402 | Growth Inhibition Assay | IC50=0.07789 μM | SANGER | ||
| HAL-01 | Growth Inhibition Assay | IC50=0.07658 μM | SANGER | ||
| KM12 | Growth Inhibition Assay | IC50=0.0764 μM | SANGER | ||
| PF-382 | Growth Inhibition Assay | IC50=0.07569 μM | SANGER | ||
| LB771-HNC | Growth Inhibition Assay | IC50=0.07549 μM | SANGER | ||
| ARH-77 | Growth Inhibition Assay | IC50=0.07278 μM | SANGER | ||
| MONO-MAC-6 | Growth Inhibition Assay | IC50=0.07212 μM | SANGER | ||
| DSH1 | Growth Inhibition Assay | IC50=0.07173 μM | SANGER | ||
| MV-4-11 | Growth Inhibition Assay | IC50=0.06995 μM | SANGER | ||
| A388 | Growth Inhibition Assay | IC50=0.06893 μM | SANGER | ||
| MLMA | Growth Inhibition Assay | IC50=0.0678 μM | SANGER | ||
| MOLT-4 | Growth Inhibition Assay | IC50=0.06605 μM | SANGER | ||
| MZ1-PC | Growth Inhibition Assay | IC50=0.06598 μM | SANGER | ||
| KLE | Growth Inhibition Assay | IC50=0.06584 μM | SANGER | ||
| CTV-1 | Growth Inhibition Assay | IC50=0.06548 μM | SANGER | ||
| Becker | Growth Inhibition Assay | IC50=0.0642 μM | SANGER | ||
| K5 | Growth Inhibition Assay | IC50=0.06403 μM | SANGER | ||
| LOUCY | Growth Inhibition Assay | IC50=0.06394 μM | SANGER | ||
| SR | Growth Inhibition Assay | IC50=0.06374 μM | SANGER | ||
| SNB75 | Growth Inhibition Assay | IC50=0.06341 μM | SANGER | ||
| A3-KAW | Growth Inhibition Assay | IC50=0.06225 μM | SANGER | ||
| LS-123 | Growth Inhibition Assay | IC50=0.05942 μM | SANGER | ||
| RS4-11 | Growth Inhibition Assay | IC50=0.05938 μM | SANGER | ||
| HC-1 | Growth Inhibition Assay | IC50=0.05827 μM | SANGER | ||
| KINGS-1 | Growth Inhibition Assay | IC50=0.05803 μM | SANGER | ||
| TGBC24TKB | Growth Inhibition Assay | IC50=0.05799 μM | SANGER | ||
| SW962 | Growth Inhibition Assay | IC50=0.05763 μM | SANGER | ||
| BB30-HNC | Growth Inhibition Assay | IC50=0.0573 μM | SANGER | ||
| IST-SL2 | Growth Inhibition Assay | IC50=0.05362 μM | SANGER | ||
| NB6 | Growth Inhibition Assay | IC50=0.05271 μM | SANGER | ||
| ALL-PO | Growth Inhibition Assay | IC50=0.04956 μM | SANGER | ||
| SK-PN-DW | Growth Inhibition Assay | IC50=0.04949 μM | SANGER | ||
| HCC1599 | Growth Inhibition Assay | IC50=0.04879 μM | SANGER | ||
| NB13 | Growth Inhibition Assay | IC50=0.04776 μM | SANGER | ||
| 8-MG-BA | Growth Inhibition Assay | IC50=0.04629 μM | SANGER | ||
| COLO-684 | Growth Inhibition Assay | IC50=0.04561 μM | SANGER | ||
| TE-15 | Growth Inhibition Assay | IC50=0.04435 μM | SANGER | ||
| SF126 | Growth Inhibition Assay | IC50=0.04361 μM | SANGER | ||
| SW982 | Growth Inhibition Assay | IC50=0.04246 μM | SANGER | ||
| Daudi | Growth Inhibition Assay | IC50=0.04225 μM | SANGER | ||
| SJSA-1 | Growth Inhibition Assay | IC50=0.04188 μM | SANGER | ||
| TK10 | Growth Inhibition Assay | IC50=0.04019 μM | SANGER | ||
| LC-1F | Growth Inhibition Assay | IC50=0.03989 μM | SANGER | ||
| HCE-4 | Growth Inhibition Assay | IC50=0.03855 μM | SANGER | ||
| TE-5 | Growth Inhibition Assay | IC50=0.03854 μM | SANGER | ||
| SW954 | Growth Inhibition Assay | IC50=0.0379 μM | SANGER | ||
| CP67-MEL | Growth Inhibition Assay | IC50=0.03759 μM | SANGER | ||
| BE-13 | Growth Inhibition Assay | IC50=0.03759 μM | SANGER | ||
| BB65-RCC | Growth Inhibition Assay | IC50=0.03691 μM | SANGER | ||
| EB-3 | Growth Inhibition Assay | IC50=0.03643 μM | SANGER | ||
| NCI-H1648 | Growth Inhibition Assay | IC50=0.03622 μM | SANGER | ||
| SH-4 | Growth Inhibition Assay | IC50=0.03582 μM | SANGER | ||
| HOP-62 | Growth Inhibition Assay | IC50=0.03556 μM | SANGER | ||
| NMC-G1 | Growth Inhibition Assay | IC50=0.03448 μM | SANGER | ||
| CCRF-CEM | Growth Inhibition Assay | IC50=0.03371 μM | SANGER | ||
| TE-10 | Growth Inhibition Assay | IC50=0.03311 μM | SANGER | ||
| NTERA-S-cl-D1 | Growth Inhibition Assay | IC50=0.03226 μM | SANGER | ||
| GAK | Growth Inhibition Assay | IC50=0.03186 μM | SANGER | ||
| CESS | Growth Inhibition Assay | IC50=0.03099 μM | SANGER | ||
| ES1 | Growth Inhibition Assay | IC50=0.03097 μM | SANGER | ||
| OVCAR-4 | Growth Inhibition Assay | IC50=0.03094 μM | SANGER | ||
| EoL-1-cell | Growth Inhibition Assay | IC50=0.02996 μM | SANGER | ||
| IA-LM | Growth Inhibition Assay | IC50=0.02917 μM | SANGER | ||
| LXF-289 | Growth Inhibition Assay | IC50=0.02872 μM | SANGER | ||
| OCI-AML2 | Growth Inhibition Assay | IC50=0.02871 μM | SANGER | ||
| DEL | Growth Inhibition Assay | IC50=0.02815 μM | SANGER | ||
| MFH-ino | Growth Inhibition Assay | IC50=0.02812 μM | SANGER | ||
| ATN-1 | Growth Inhibition Assay | IC50=0.02789 μM | SANGER | ||
| Ramos-2G6-4C10 | Growth Inhibition Assay | IC50=0.0275 μM | SANGER | ||
| MC116 | Growth Inhibition Assay | IC50=0.0268 μM | SANGER | ||
| GR-ST | Growth Inhibition Assay | IC50=0.02655 μM | SANGER | ||
| A101D | Growth Inhibition Assay | IC50=0.0265 μM | SANGER | ||
| MMAC-SF | Growth Inhibition Assay | IC50=0.02633 μM | SANGER | ||
| MZ7-mel | Growth Inhibition Assay | IC50=0.02609 μM | SANGER | ||
| TE-12 | Growth Inhibition Assay | IC50=0.0255 μM | SANGER | ||
| LB1047-RCC | Growth Inhibition Assay | IC50=0.02488 μM | SANGER | ||
| GI-1 | Growth Inhibition Assay | IC50=0.02469 μM | SANGER | ||
| HD-MY-Z | Growth Inhibition Assay | IC50=0.02426 μM | SANGER | ||
| KURAMOCHI | Growth Inhibition Assay | IC50=0.02414 μM | SANGER | ||
| TE-11 | Growth Inhibition Assay | IC50=0.02372 μM | SANGER | ||
| MZ2-MEL | Growth Inhibition Assay | IC50=0.02337 μM | SANGER | ||
| NCI-H747 | Growth Inhibition Assay | IC50=0.0233 μM | SANGER | ||
| OS-RC-2 | Growth Inhibition Assay | IC50=0.02323 μM | SANGER | ||
| SK-UT-1 | Growth Inhibition Assay | IC50=0.02261 μM | SANGER | ||
| SW872 | Growth Inhibition Assay | IC50=0.02247 μM | SANGER | ||
| BV-173 | Growth Inhibition Assay | IC50=0.02203 μM | SANGER | ||
| LAMA-84 | Growth Inhibition Assay | IC50=0.02167 μM | SANGER | ||
| LC-2-ad | Growth Inhibition Assay | IC50=0.02137 μM | SANGER | ||
| ES8 | Growth Inhibition Assay | IC50=0.01844 μM | SANGER | ||
| MHH-PREB-1 | Growth Inhibition Assay | IC50=0.01818 μM | SANGER | ||
| IST-MEL1 | Growth Inhibition Assay | IC50=0.01774 μM | SANGER | ||
| NCI-H1355 | Growth Inhibition Assay | IC50=0.01646 μM | SANGER | ||
| HEL | Growth Inhibition Assay | IC50=0.0164 μM | SANGER | ||
| MOLT-16 | Growth Inhibition Assay | IC50=0.01622 μM | SANGER | ||
| no-10 | Growth Inhibition Assay | IC50=0.01584 μM | SANGER | ||
| KE-37 | Growth Inhibition Assay | IC50=0.01573 μM | SANGER | ||
| GB-1 | Growth Inhibition Assay | IC50=0.01553 μM | SANGER | ||
| CMK | Growth Inhibition Assay | IC50=0.01543 μM | SANGER | ||
| RKO | Growth Inhibition Assay | IC50=0.01533 μM | SANGER | ||
| ACN | Growth Inhibition Assay | IC50=0.015 μM | SANGER | ||
| HT-144 | Growth Inhibition Assay | IC50=0.01451 μM | SANGER | ||
| LS-411N | Growth Inhibition Assay | IC50=0.01439 μM | SANGER | ||
| SK-LMS-1 | Growth Inhibition Assay | IC50=0.01413 μM | SANGER | ||
| PSN1 | Growth Inhibition Assay | IC50=0.01409 μM | SANGER | ||
| no-11 | Growth Inhibition Assay | IC50=0.01395 μM | SANGER | ||
| KS-1 | Growth Inhibition Assay | IC50=0.01392 μM | SANGER | ||
| KGN | Growth Inhibition Assay | IC50=0.01359 μM | SANGER | ||
| A4-Fuk | Growth Inhibition Assay | IC50=0.01319 μM | SANGER | ||
| KU812 | Growth Inhibition Assay | IC50=0.01296 μM | SANGER | ||
| OPM-2 | Growth Inhibition Assay | IC50=0.0129 μM | SANGER | ||
| KALS-1 | Growth Inhibition Assay | IC50=0.01198 μM | SANGER | ||
| EW-16 | Growth Inhibition Assay | IC50=0.01192 μM | SANGER | ||
| LB996-RCC | Growth Inhibition Assay | IC50=0.01137 μM | SANGER | ||
| NALM-6 | Growth Inhibition Assay | IC50=0.01102 μM | SANGER | ||
| ES6 | Growth Inhibition Assay | IC50=0.01083 μM | SANGER | ||
| GCIY | Growth Inhibition Assay | IC50=0.01007 μM | SANGER | ||
| VA-ES-BJ | Growth Inhibition Assay | IC50=0.00984 μM | SANGER | ||
| HUTU-80 | Growth Inhibition Assay | IC50=0.00929 μM | SANGER | ||
| EVSA-T | Growth Inhibition Assay | IC50=0.00892 μM | SANGER | ||
| EW-1 | Growth Inhibition Assay | IC50=0.00749 μM | SANGER | ||
| MRK-nu-1 | Growth Inhibition Assay | IC50=0.00707 μM | SANGER | ||
| BL-41 | Growth Inhibition Assay | IC50=0.00696 μM | SANGER | ||
| RPMI-8226 | Growth Inhibition Assay | IC50=0.00651 μM | SANGER | ||
| 697 | Growth Inhibition Assay | IC50=0.00634 μM | SANGER | ||
| TGBC1TKB | Growth Inhibition Assay | IC50=0.00379 μM | SANGER | ||
| BL-70 | Growth Inhibition Assay | IC50=0.00337 μM | SANGER | ||
| RL95-2 | Growth Inhibition Assay | IC50=0.0032 μM | SANGER | ||
| L-363 | Growth Inhibition Assay | IC50=0.00284 μM | SANGER | ||
| NOS-1 | Growth Inhibition Assay | IC50=0.00242 μM | SANGER | ||
| DOHH-2 | Growth Inhibition Assay | IC50=0.00225 μM | SANGER | ||
| OCUB-M | Growth Inhibition Assay | IC50=0.00194 μM | SANGER | ||
| ETK-1 | Growth Inhibition Assay | IC50=0.000306 μM | SANGER | ||
| OMC-1 | Growth Inhibition Assay | IC50=0.07804 μM | SANGER | ||
| HT | Growth Inhibition Assay | IC50=0.08 μM | SANGER | ||
| QIMR-WIL | Growth Inhibition Assay | IC50=0.08055 μM | SANGER | ||
| KNS-42 | Growth Inhibition Assay | IC50=0.08203 μM | SANGER | ||
| BC-3 | Growth Inhibition Assay | IC50=0.08204 μM | SANGER | ||
| ES4 | Growth Inhibition Assay | IC50=0.08296 μM | SANGER | ||
| NCI-H23 | Growth Inhibition Assay | IC50=0.0844 μM | SANGER | ||
| LB2518-MEL | Growth Inhibition Assay | IC50=0.08696 μM | SANGER | ||
| LC4-1 | Growth Inhibition Assay | IC50=0.08738 μM | SANGER | ||
| SIG-M5 | Growth Inhibition Assay | IC50=0.08904 μM | SANGER | ||
| LP-1 | Growth Inhibition Assay | IC50=0.08938 μM | SANGER | ||
| TE-8 | Growth Inhibition Assay | IC50=0.09016 μM | SANGER | ||
| K-562 | Growth Inhibition Assay | IC50=0.0912 μM | SANGER | ||
| J-RT3-T3-5 | Growth Inhibition Assay | IC50=0.09153 μM | SANGER | ||
| DU-4475 | Growth Inhibition Assay | IC50=0.09165 μM | SANGER | ||
| RXF393 | Growth Inhibition Assay | IC50=0.09462 μM | SANGER | ||
| NB5 | Growth Inhibition Assay | IC50=0.09625 μM | SANGER | ||
| A704 | Growth Inhibition Assay | IC50=0.09741 μM | SANGER | ||
| SF268 | Growth Inhibition Assay | IC50=0.09825 μM | SANGER | ||
| UACC-257 | Growth Inhibition Assay | IC50=0.09833 μM | SANGER | ||
| L-428 | Growth Inhibition Assay | IC50=0.1032 μM | SANGER | ||
| ST486 | Growth Inhibition Assay | IC50=0.10606 μM | SANGER | ||
| SK-MM-2 | Growth Inhibition Assay | IC50=0.10709 μM | SANGER | ||
| COR-L279 | Growth Inhibition Assay | IC50=0.10832 μM | SANGER | ||
| JiyoyeP-2003 | Growth Inhibition Assay | IC50=0.10843 μM | SANGER | ||
| GT3TKB | Growth Inhibition Assay | IC50=0.10918 μM | SANGER | ||
| HCE-T | Growth Inhibition Assay | IC50=0.1094 μM | SANGER | ||
| SBC-1 | Growth Inhibition Assay | IC50=0.11119 μM | SANGER | ||
| KNS-81-FD | Growth Inhibition Assay | IC50=0.11273 μM | SANGER | ||
| NCI-H2126 | Growth Inhibition Assay | IC50=0.11394 μM | SANGER | ||
| EM-2 | Growth Inhibition Assay | IC50=0.11529 μM | SANGER | ||
| JAR | Growth Inhibition Assay | IC50=0.11598 μM | SANGER | ||
| NB17 | Growth Inhibition Assay | IC50=0.11652 μM | SANGER | ||
| LB647-SCLC | Growth Inhibition Assay | IC50=0.11705 μM | SANGER | ||
| CAL-148 | Growth Inhibition Assay | IC50=0.118 μM | SANGER | ||
| K052 | Growth Inhibition Assay | IC50=0.11851 μM | SANGER | ||
| KARPAS-299 | Growth Inhibition Assay | IC50=0.12233 μM | SANGER | ||
| KARPAS-422 | Growth Inhibition Assay | IC50=0.12432 μM | SANGER | ||
| LOXIMVI | Growth Inhibition Assay | IC50=0.12515 μM | SANGER | ||
| SCC-3 | Growth Inhibition Assay | IC50=0.12851 μM | SANGER | ||
| LU-139 | Growth Inhibition Assay | IC50=0.12975 μM | SANGER | ||
| COLO-320-HSR | Growth Inhibition Assay | IC50=0.13086 μM | SANGER | ||
| ONS-76 | Growth Inhibition Assay | IC50=0.1313 μM | SANGER | ||
| U-266 | Growth Inhibition Assay | IC50=0.13245 μM | SANGER | ||
| HH | Growth Inhibition Assay | IC50=0.13535 μM | SANGER | ||
| BB49-HNC | Growth Inhibition Assay | IC50=0.13587 μM | SANGER | ||
| NCI-SNU-5 | Growth Inhibition Assay | IC50=0.13596 μM | SANGER | ||
| C2BBe1 | Growth Inhibition Assay | IC50=0.13746 μM | SANGER | ||
| ML-2 | Growth Inhibition Assay | IC50=0.13953 μM | SANGER | ||
| SCH | Growth Inhibition Assay | IC50=0.13974 μM | SANGER | ||
| NCI-H82 | Growth Inhibition Assay | IC50=0.14104 μM | SANGER | ||
| MDA-MB-134-VI | Growth Inhibition Assay | IC50=0.14252 μM | SANGER | ||
| SK-N-DZ | Growth Inhibition Assay | IC50=0.14719 μM | SANGER | ||
| HL-60 | Growth Inhibition Assay | IC50=0.1492 μM | SANGER | ||
| LB373-MEL-D | Growth Inhibition Assay | IC50=0.15539 μM | SANGER | ||
| LB831-BLC | Growth Inhibition Assay | IC50=0.16076 μM | SANGER | ||
| SK-NEP-1 | Growth Inhibition Assay | IC50=0.16101 μM | SANGER | ||
| IMR-5 | Growth Inhibition Assay | IC50=0.16483 μM | SANGER | ||
| Calu-6 | Growth Inhibition Assay | IC50=0.16537 μM | SANGER | ||
| NCI-H1882 | Growth Inhibition Assay | IC50=0.16728 μM | SANGER | ||
| NCI-H446 | Growth Inhibition Assay | IC50=0.16891 μM | SANGER | ||
| GI-ME-N | Growth Inhibition Assay | IC50=0.1802 μM | SANGER | ||
| MPP-89 | Growth Inhibition Assay | IC50=0.18121 μM | SANGER | ||
| CGTH-W-1 | Growth Inhibition Assay | IC50=0.18436 μM | SANGER | ||
| MEG-01 | Growth Inhibition Assay | IC50=0.18628 μM | SANGER | ||
| NCI-H748 | Growth Inhibition Assay | IC50=0.18911 μM | SANGER | ||
| COLO-829 | Growth Inhibition Assay | IC50=0.20673 μM | SANGER | ||
| GOTO | Growth Inhibition Assay | IC50=0.2073 μM | SANGER | ||
| EW-3 | Growth Inhibition Assay | IC50=0.21718 μM | SANGER | ||
| KY821 | Growth Inhibition Assay | IC50=0.22045 μM | SANGER | ||
| CTB-1 | Growth Inhibition Assay | IC50=0.22228 μM | SANGER | ||
| TALL-1 | Growth Inhibition Assay | IC50=0.22921 μM | SANGER | ||
| NCI-H209 | Growth Inhibition Assay | IC50=0.22962 μM | SANGER | ||
| IST-MES1 | Growth Inhibition Assay | IC50=0.2351 μM | SANGER | ||
| DMS-114 | Growth Inhibition Assay | IC50=0.23993 μM | SANGER | ||
| SNU-C2B | Growth Inhibition Assay | IC50=0.24316 μM | SANGER | ||
| DG-75 | Growth Inhibition Assay | IC50=0.26195 μM | SANGER | ||
| D-336MG | Growth Inhibition Assay | IC50=0.26557 μM | SANGER | ||
| KMOE-2 | Growth Inhibition Assay | IC50=0.27538 μM | SANGER | ||
| NB1 | Growth Inhibition Assay | IC50=0.27926 μM | SANGER | ||
| RH-1 | Growth Inhibition Assay | IC50=0.27989 μM | SANGER | ||
| TE-1 | Growth Inhibition Assay | IC50=0.28479 μM | SANGER | ||
| RPMI-6666 | Growth Inhibition Assay | IC50=0.2884 μM | SANGER | ||
| TE-9 | Growth Inhibition Assay | IC50=0.28985 μM | SANGER | ||
| BT-474 | Growth Inhibition Assay | IC50=0.29334 μM | SANGER | ||
| COLO-668 | Growth Inhibition Assay | IC50=0.29429 μM | SANGER | ||
| NCI-H1581 | Growth Inhibition Assay | IC50=0.29689 μM | SANGER | ||
| MN-60 | Growth Inhibition Assay | IC50=0.29905 μM | SANGER | ||
| L-540 | Growth Inhibition Assay | IC50=0.30081 μM | SANGER | ||
| MOLT-13 | Growth Inhibition Assay | IC50=0.30183 μM | SANGER | ||
| NCI-SNU-16 | Growth Inhibition Assay | IC50=0.30205 μM | SANGER | ||
| NCI-H64 | Growth Inhibition Assay | IC50=0.30331 μM | SANGER | ||
| CP66-MEL | Growth Inhibition Assay | IC50=0.31415 μM | SANGER | ||
| NB69 | Growth Inhibition Assay | IC50=0.32188 μM | SANGER | ||
| NCI-H2196 | Growth Inhibition Assay | IC50=0.33036 μM | SANGER | ||
| MHH-CALL-2 | Growth Inhibition Assay | IC50=0.33468 μM | SANGER | ||
| HCC2157 | Growth Inhibition Assay | IC50=0.33954 μM | SANGER | ||
| LS-1034 | Growth Inhibition Assay | IC50=0.36388 μM | SANGER | ||
| BOKU | Growth Inhibition Assay | IC50=0.37199 μM | SANGER | ||
| GDM-1 | Growth Inhibition Assay | IC50=0.37234 μM | SANGER | ||
| TGW | Growth Inhibition Assay | IC50=0.37453 μM | SANGER | ||
| SNU-C1 | Growth Inhibition Assay | IC50=0.38215 μM | SANGER | ||
| LS-513 | Growth Inhibition Assay | IC50=0.39071 μM | SANGER | ||
| NOMO-1 | Growth Inhibition Assay | IC50=0.39971 μM | SANGER | ||
| MS-1 | Growth Inhibition Assay | IC50=0.40177 μM | SANGER | ||
| EKVX | Growth Inhibition Assay | IC50=0.44461 μM | SANGER | ||
| SK-MEL-2 | Growth Inhibition Assay | IC50=0.464 μM | SANGER | ||
| HCC1187 | Growth Inhibition Assay | IC50=0.46768 μM | SANGER | ||
| HCC2218 | Growth Inhibition Assay | IC50=0.47227 μM | SANGER | ||
| NCI-H187 | Growth Inhibition Assay | IC50=0.4784 μM | SANGER | ||
| DB | Growth Inhibition Assay | IC50=0.50055 μM | SANGER | ||
| NCI-H69 | Growth Inhibition Assay | IC50=0.50247 μM | SANGER | ||
| NCI-H1694 | Growth Inhibition Assay | IC50=0.50697 μM | SANGER | ||
| CA46 | Growth Inhibition Assay | IC50=0.50917 μM | SANGER | ||
| EHEB | Growth Inhibition Assay | IC50=0.51193 μM | SANGER | ||
| NCCIT | Growth Inhibition Assay | IC50=0.54321 μM | SANGER | ||
| NCI-H720 | Growth Inhibition Assay | IC50=0.54962 μM | SANGER | ||
| LU-134-A | Growth Inhibition Assay | IC50=0.55296 μM | SANGER | ||
| MC-CAR | Growth Inhibition Assay | IC50=0.55431 μM | SANGER | ||
| NEC8 | Growth Inhibition Assay | IC50=0.56172 μM | SANGER | ||
| NCI-H524 | Growth Inhibition Assay | IC50=0.58748 μM | SANGER | ||
| NCI-H1963 | Growth Inhibition Assay | IC50=0.6258 μM | SANGER | ||
| SU-DHL-1 | Growth Inhibition Assay | IC50=0.64526 μM | SANGER | ||
| NCI-H1522 | Growth Inhibition Assay | IC50=0.64883 μM | SANGER | ||
| NCI-H1155 | Growth Inhibition Assay | IC50=0.6535 μM | SANGER | ||
| D-263MG | Growth Inhibition Assay | IC50=0.66101 μM | SANGER | ||
| TE-6 | Growth Inhibition Assay | IC50=0.68104 μM | SANGER | ||
| NCI-H1417 | Growth Inhibition Assay | IC50=0.69711 μM | SANGER | ||
| NCI-H510A | Growth Inhibition Assay | IC50=0.77236 μM | SANGER | ||
| KARPAS-45 | Growth Inhibition Assay | IC50=0.78235 μM | SANGER | ||
| IST-SL1 | Growth Inhibition Assay | IC50=0.85825 μM | SANGER | ||
| COLO-824 | Growth Inhibition Assay | IC50=0.87835 μM | SANGER | ||
| NCI-H526 | Growth Inhibition Assay | IC50=0.90958 μM | SANGER | ||
| NCI-H719 | Growth Inhibition Assay | IC50=0.96657 μM | SANGER | ||
| SF539 | Growth Inhibition Assay | IC50=0.98396 μM | SANGER | ||
| NKM-1 | Growth Inhibition Assay | IC50=0.98663 μM | SANGER | ||
| A253 | Growth Inhibition Assay | IC50=0.98885 μM | SANGER | ||
| TE-441-T | Growth Inhibition Assay | IC50=1.0435 μM | SANGER | ||
| COR-L88 | Growth Inhibition Assay | IC50=1.11354 μM | SANGER | ||
| CPC-N | Growth Inhibition Assay | IC50=1.15888 μM | SANGER | ||
| NCI-H2107 | Growth Inhibition Assay | IC50=1.17261 μM | SANGER | ||
| ECC4 | Growth Inhibition Assay | IC50=1.18693 μM | SANGER | ||
| NCI-H2141 | Growth Inhibition Assay | IC50=1.19299 μM | SANGER | ||
| NCI-H1770 | Growth Inhibition Assay | IC50=1.32928 μM | SANGER | ||
| NCI-SNU-1 | Growth Inhibition Assay | IC50=1.3304 μM | SANGER | ||
| LU-165 | Growth Inhibition Assay | IC50=1.45467 μM | SANGER | ||
| Raji | Growth Inhibition Assay | IC50=1.47259 μM | SANGER | ||
| EW-24 | Growth Inhibition Assay | IC50=1.54619 μM | SANGER | ||
| KP-N-RT-BM-1 | Growth Inhibition Assay | IC50=1.56551 μM | SANGER | ||
| SK-MEL-1 | Growth Inhibition Assay | IC50=1.64877 μM | SANGER | ||
| SCLC-21H | Growth Inhibition Assay | IC50=1.66651 μM | SANGER | ||
| SIMA | Growth Inhibition Assay | IC50=1.68233 μM | SANGER | ||
| NCI-H1304 | Growth Inhibition Assay | IC50=1.70765 μM | SANGER | ||
| REH | Growth Inhibition Assay | IC50=1.71078 μM | SANGER | ||
| KP-N-YS | Growth Inhibition Assay | IC50=1.93551 μM | SANGER | ||
| DMS-153 | Growth Inhibition Assay | IC50=1.9759 μM | SANGER | ||
| NB10 | Growth Inhibition Assay | IC50=2.20878 μM | SANGER | ||
| NCI-H345 | Growth Inhibition Assay | IC50=2.61905 μM | SANGER | ||
| SK-N-FI | Growth Inhibition Assay | IC50=2.75381 μM | SANGER | ||
| NCI-H1092 | Growth Inhibition Assay | IC50=2.95394 μM | SANGER | ||
| KM-H2 | Growth Inhibition Assay | IC50=3.65396 μM | SANGER | ||
| KG-1 | Growth Inhibition Assay | IC50=3.71688 μM | SANGER | ||
| D-283MED | Growth Inhibition Assay | IC50=3.7587 μM | SANGER | ||
| LU-65 | Growth Inhibition Assay | IC50=4.11485 μM | SANGER | ||
| MSTO-211H | Growth Inhibition Assay | IC50=4.18898 μM | SANGER | ||
| NCI-H2081 | Growth Inhibition Assay | IC50=4.29174 μM | SANGER | ||
| D-247MG | Growth Inhibition Assay | IC50=4.43191 μM | SANGER | ||
| P31-FUJ | Growth Inhibition Assay | IC50=4.45019 μM | SANGER | ||
| DMS-79 | Growth Inhibition Assay | IC50=4.93183 μM | SANGER | ||
| KASUMI-1 | Growth Inhibition Assay | IC50=4.97509 μM | SANGER | ||
| JVM-3 | Growth Inhibition Assay | IC50=5.25014 μM | SANGER | ||
| LAN-6 | Growth Inhibition Assay | IC50=5.51736 μM | SANGER | ||
| DJM-1 | Growth Inhibition Assay | IC50=5.56029 μM | SANGER | ||
| JVM-2 | Growth Inhibition Assay | IC50=5.67092 μM | SANGER | ||
| U-87-MG | Growth Inhibition Assay | IC50=5.89964 μM | SANGER | ||
| SHP-77 | Growth Inhibition Assay | IC50=5.94928 μM | SANGER | ||
| RPMI-8866 | Growth Inhibition Assay | IC50=6.27013 μM | SANGER | ||
| ES7 | Growth Inhibition Assay | IC50=6.39924 μM | SANGER | ||
| WSU-NHL | Growth Inhibition Assay | IC50=6.51609 μM | SANGER | ||
| CAS-1 | Growth Inhibition Assay | IC50=7.09749 μM | SANGER | ||
| MHH-NB-11 | Growth Inhibition Assay | IC50=8.23152 μM | SANGER | ||
| ES5 | Growth Inhibition Assay | IC50=9.52286 μM | SANGER | ||
| HDLM-2 | Growth Inhibition Assay | IC50=9.95902 μM | SANGER | ||
| NCI-H2171 | Growth Inhibition Assay | IC50=10.5927 μM | SANGER | ||
| LNCaP-Clone-FGC | Growth Inhibition Assay | IC50=11.3718 μM | SANGER | ||
| NCI-H1395 | Growth Inhibition Assay | IC50=12.2699 μM | SANGER | ||
| NCI-H1436 | Growth Inhibition Assay | IC50=13.3195 μM | SANGER | ||
| RCC10RGB | Growth Inhibition Assay | IC50=13.3221 μM | SANGER | ||
| SW684 | Growth Inhibition Assay | IC50=13.9797 μM | SANGER | ||
| NCI-H1650 | Growth Inhibition Assay | IC50=15.0447 μM | SANGER | ||
| EW-13 | Growth Inhibition Assay | IC50=15.2021 μM | SANGER | ||
| EC-GI-10 | Growth Inhibition Assay | IC50=15.8644 μM | SANGER | ||
| CHP-126 | Growth Inhibition Assay | IC50=17.1918 μM | SANGER | ||
| NCI-H226 | Growth Inhibition Assay | IC50=18.0403 μM | SANGER | ||
| NCI-H1838 | Growth Inhibition Assay | IC50=20.1848 μM | SANGER | ||
| EW-11 | Growth Inhibition Assay | IC50=24.2718 μM | SANGER | ||
| EB2 | Growth Inhibition Assay | IC50=31.5318 μM | SANGER | ||
| D-502MG | Growth Inhibition Assay | IC50=43.9229 μM | SANGER | ||
| U-698-M | Growth Inhibition Assay | IC50=48.8671 μM | SANGER | ||
| ES3 | Growth Inhibition Assay | IC50=49.3893 μM | SANGER | ||
| PC3 | Function assay | Inhibition of PI3Kalpha in human PC3 cells expressing Akt1 S473D mutant assessed as phosphorylation of Akt Thr308 by immunoblotting, EC50 = 1.8 μM. | 21322566 | ||
| TC32 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for TC32 cells | 29435139 | ||
| A673 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for A673 cells | 29435139 | ||
| BT-37 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for BT-37 cells | 29435139 | ||
| OHS-50 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for OHS-50 cells | 29435139 | ||
| Rh41 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for Rh41 cells | 29435139 | ||
| SJ-GBM2 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SJ-GBM2 cells | 29435139 | ||
| SK-N-MC | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SK-N-MC cells | 29435139 | ||
| NB-EBc1 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for NB-EBc1 cells | 29435139 | ||
| SK-N-MC | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for SK-N-MC cells | 29435139 | ||
| TC32 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for TC32 cells | 29435139 | ||
| 點(diǎn)擊查看更多細(xì)胞系數(shù)據(jù) | |||||
生物活性
| 產(chǎn)品描述 | Torin 1是一種有效的mTORC1/2抑制劑，在無細(xì)胞試驗(yàn)中IC50為2 nM/10 nM；作用于mTOR比作用于PI3K選擇性高1000倍。 | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 靶點(diǎn) |
|
| 體外研究(In Vitro) | ||||
| 體外研究活性 | Torin1在2和10 nM濃度下分別抑制mTORC1 和mTORC2底物的磷酸化。此外，Torin1對mTOR比對PI3K (EC50 = 1800 nM)的選擇性高1000倍，比對450種其它蛋白激酶的結(jié)合選擇性高100倍。[1] [2] Torin1通過耐rapamycin機(jī)制引起細(xì)胞周期阻滯，并且其不依賴于mTORC2。Torin1比rapamycin更完全地干擾mTORC1依賴表現(xiàn)型。Cap依賴性翻譯需要mTORC1耐Rapamycin的功能。[1]在近期的一項(xiàng)研究中，據(jù)報(bào)道Torin1通過活化人內(nèi)分泌細(xì)胞系BON 中MEK/ERK/c-Jun通路，能夠增加神經(jīng)降壓素分泌和基因表達(dá)。[3] | |||
|---|---|---|---|---|
| 激酶實(shí)驗(yàn) | mTORC1和 mTORC2體外激酶試驗(yàn) | |||
| 為產(chǎn)生可溶性mTORC1，HEK-293T細(xì)胞系，穩(wěn)定表達(dá)N端FLAG標(biāo)記的Raptor，使用水泡性口炎病毒G-假型病毒MSCV逆轉(zhuǎn)錄酶病毒產(chǎn)生。對于mTORC2，產(chǎn)生相似的HeLa細(xì)胞，能夠穩(wěn)定表達(dá)N端FLAG-標(biāo)記的Protor-1。兩個(gè)復(fù)合物通過在50 mm HEPES，pH 7.4，10 mm 焦磷酸鈉，10 mm β-甘油磷酸鈉，100 mm NaCl，2 mm EDTA，0.3% CHAPS中裂解細(xì)胞純化。細(xì)胞在4 °C下裂解30分鐘，不溶解部分通過以13,000 rpm微量離心10分鐘移除。上清液與FLAG-M2單克隆抗體-瓊脂糖培育1小時(shí)，然后用裂解緩沖液洗滌3次，用包含終濃度0.5 M NaCl的裂解緩沖液洗滌1次。純化的mTORC1用100 μg/mL 3×FLAG多肽在50 mm HEPES，pH 7.4，100 mm NaCl中洗脫。將洗出液等分收集，儲存在-80 °C。純化底物S6K1 和 Akt1。激酶試驗(yàn)在30 °C，由激酶緩沖液(25 mm HEPES，pH 7.4，50 mm KCl，10 mm MgCl2，500 μm ATP)和150 ng 滅活的S6K1或Akt1底物組成的20 μL終體積混合物中進(jìn)行。反應(yīng)通過加入80 μL樣品緩沖液停止，并沸煮5分鐘。隨后，樣品通過SDS-PAGE和免疫印跡法分析。 | ||||
| 細(xì)胞實(shí)驗(yàn) | 細(xì)胞系 | MEFs | ||
| 濃度 | ~250 nM | |||
| 孵育時(shí)間 | 4天 | |||
| 方法 | 細(xì)胞活性通過CellTiter-Glo發(fā)光法細(xì)胞活性測定評估。在第0天，細(xì)胞以500細(xì)胞每孔接種到96孔板，并生長過夜。第1天，細(xì)胞用適當(dāng)?shù)幕衔锾幚?，隨后在第3-5天分析。對于分析，板在室溫下培育60分鐘，將50 μL CellTiter-Glo試劑加入每孔中，板在定軌搖床上混合12分鐘。發(fā)光在標(biāo)準(zhǔn)平板光度計(jì)上定量。 | |||
| 實(shí)驗(yàn)圖片 | 檢測方法 | 檢測指標(biāo) | 實(shí)驗(yàn)圖片 | PMID |
| Western blot | Vinculin / p-S6 p-mTOR(S2481) / mTOR / rictor / Sin1 / p-Akt S473 / p-Akt T450 / p-Akt T308 / p-S6K1 T389 / S6K1 LC3 / p62 / PARP / pAKT(S473) / p4EBP1(S65) / pS6K1(T389) / pS6(S235/236) ATF4 / p-S6K(T389) / S6K / p-eIF2α(S51) / eIF2α / 4EBP1 / eIF3b |
|
28757207 | |
| Immunofluorescence | lipin-1 |
|
28347999 | |
| Growth inhibition assay | Cell viability |
|
28757207 | |
| 體內(nèi)研究(In Vivo) | ||
| 體內(nèi)研究活性 | Torin1在20 mg/kg劑量下，在U87MG異種移植模型中是有效的，并且在腫瘤和外周組織中對mTOR下游效應(yīng)蛋白表現(xiàn)出良好的藥效學(xué)抑制作用。[2] | |
|---|---|---|
| 動物實(shí)驗(yàn) | Animal Models | U87MG 異種移植物模型 |
| Dosages | 20 mg/kg | |
| Administration | 腹腔注射給藥，每天一次 | |
化學(xué)信息&溶解度
| 分子量 | 607.62 | 分子式 | C35H28F3N5O2 |
| CAS號 | 1222998-36-8 | SDF | Download Torin 1 SDF |
| Smiles | CCC(=O)N1CCN(CC1)C2=C(C=C(C=C2)N3C(=O)C=CC4=CN=C5C=CC(=CC5=C43)C6=CC7=CC=CC=C7N=C6)C(F)(F)F | ||
| 儲存條件(自收到貨起) | |||
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體外溶解度 |
DMSO : 3 mg/mL ( (4.93 mM) ；DMSO吸濕會降低化合物溶解度，請使用新開封DMSO) Water : Insoluble Ethanol : Insoluble |
摩爾濃度計(jì)算器 |
|
體內(nèi)溶解度 現(xiàn)配現(xiàn)用，請按從左到右的順序依次添加，澄清后再加入下一溶劑 |
動物體內(nèi)配方計(jì)算器 | ||||
實(shí)驗(yàn)計(jì)算
摩爾濃度計(jì)算器
動物體內(nèi)配方計(jì)算器（澄清溶液）
第一步：請輸入基本實(shí)驗(yàn)信息（考慮到實(shí)驗(yàn)過程中的損耗，建議多配一只動物的藥量）
mg/kg
g
μL
只
第二步：請輸入動物體內(nèi)配方組成（配方適用于不溶于水的藥物；不同批次藥物配方比例不同，請聯(lián)系Selleck為您提供正確的澄清溶液配方）
% DMSO
%
% Tween 80
% ddH2O
%DMSO
%
計(jì)算結(jié)果：
工作液濃度： mg/ml；
DMSO母液配制方法： mg 藥物溶于μL DMSO溶液（母液濃度mg/mL，注：如該濃度超過該批次藥物DMSO溶解度，請先聯(lián)系Selleck）；
體內(nèi)配方配制方法：取μL DMSO母液，加入μL PEG300，混勻澄清后加入μL Tween 80，混勻澄清后加入μL ddH2O，混勻澄清。
體內(nèi)配方配制方法：取μL DMSO母液，加入μL Corn oil，混勻澄清。
注意：1. 首先保證母液是澄清的；
2.一定要按照順序依次將溶劑加入，進(jìn)行下一步操作之前必須保證上一步操作得到的是澄清的溶液，可采用渦旋、超聲或水浴加熱等物理方法助溶。
技術(shù)支持
在訂購、運(yùn)輸、儲存和使用我們的產(chǎn)品的任何階段，您遇到的任何問題，均可以通過撥打我們的熱線電話400-668-6834，或者技術(shù)支持郵箱tech@selleck.cn，直接聯(lián)系到我們。我們會在24小時(shí)內(nèi)盡快聯(lián)系您。
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常見問題及建議解決方法
問題 1:
How to re-constitute the inhibitor for i.p. administration?
回答：
S2827 Torin 1 can be dissolved in 5% DMSO/30% PEG 300/ddH2O at 0.25 mg/ml clearly, and in 10% DMSO/corn oil at 0.5 mg/ml, it is also a clear solution.
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